LOCUS       sequence-190956-        1966 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  empty BB1 for cds cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1966)
  AUTHORS   Prielhofer R, Barrero JJ, Steuer S, Gassler T, Zahrl R, Baumann K, 
            Sauer M, Mattanovich D, Gasser B, Marx H
  TITLE     GoldenPiCS: a Golden Gate-derived modular cloning system for applied
            synthetic biology in the yeast Pichia pastoris.
  JOURNAL   BMC Syst Biol. 2017 Dec 8;11(1):123. doi: 10.1186/s12918-017-0492-3.
  PUBMED    29221460
REFERENCE   2  (bases 1 to 1966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Syst 
            Biol."; date: "2017-12-8"; volume: "11(1):123. doi"; pages: " 
            10.1186/s12918-017-0492-3"
COMMENT     Sequence Label: BB1_23
FEATURES             Location/Qualifiers
     source          1..1966
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      758..785
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(797..1384)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(877..896)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1424..1441
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1425..1441
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1686..1702)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1686..1702)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      1750..1781
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        1782..1884
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             join(1885..1966,1..734)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCLKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(join(1958..1966,1..11))
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 gggctcgcga taatgtcggg caatcaggtg cgacaatcta tcgattgtat gggaagcccg
       61 atgcgccaga gttgtttctg aaacatggca aaggtagcgt tgccaatgat gttacagatg
      121 agatggtcag gctaaactgg ctgacggaat ttatgcctct tccgaccatc aagcatttta
      181 tccgtactcc tgatgatgca tggttactca ccactgcgat cccagggaaa acagcattcc
      241 aggtattaga agaatatcct gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc
      301 tgcgccggtt gcattcgatt cctgtttgta attgtccttt taacggcgat cgcgtatttc
      361 gtctcgctca ggcgcaatca cgaatgaata acggtttggt tggtgcgagt gattttgatg
      421 acgagcgtaa tggctggcct gttgaacaag tctggaaaga aatgcataaa ctcttgccat
      481 tctcaccgga ttcagtcgtc actcatggtg atttctcact tgataacctt atttttgacg
      541 aggggaaatt aataggttgt attgatgttg gacgagtcgg aatcgcagac cgataccagg
      601 atcttgccat cctatggaac tgcctcggtg agttttctcc ttcattacag aaacggcttt
      661 ttcaaaaata tggtattgat aatcctgata tgaataaatt gcagtttcac ttgatgctcg
      721 atgagttttt ctaatgagga cctaaatgta atcacctggc tcaccttcgg gtgggccttt
      781 ctgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgat
      841 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
      901 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
      961 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     1021 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     1081 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     1141 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     1201 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     1261 tgctgaagcc agttacctcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     1321 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     1381 tcaagaagat cctttgattt tctaccgaag aaaggcccac ccgtgtaaaa cgacggccag
     1441 tttatctagt cagcttgatt ctagctgatc gtggaccgga aggtgagcca gtgagttgat
     1501 tgcagtccag ttacgctgga gtctgaggct cgtcctgaat gatatgcggc ctcgaattcg
     1561 aagacgcaag ctgagaccgg atccatctcg agggtctcac atggcgtctt cgaattcgcg
     1621 cgtgatctta cggcattata cgtatgatcg gtccacgatc agctagatta tctagtcagc
     1681 ttgatgtcat agctgtttcc tgaggctcaa tactgaccat ttaaatcata cctgacctcc
     1741 atagcagaaa gtcaaaagcc tccgaccgga ggcttttgac ttgatcggca cgtaagaggt
     1801 tccaactttc accataatga aataagatca ctaccgggcg tattttttga gttatcgaga
     1861 ttttcaggag ctaaggaagc taaaatgagc catattcaac gggaaacgtc ttgcttgaag
     1921 ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaat
//
