LOCUS       sequence-190960-        2337 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BB1 with insert: promoter PGPM1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Prielhofer R, Barrero JJ, Steuer S, Gassler T, Zahrl R, Baumann K, 
            Sauer M, Mattanovich D, Gasser B, Marx H
  TITLE     GoldenPiCS: a Golden Gate-derived modular cloning system for applied
            synthetic biology in the yeast Pichia pastoris.
  JOURNAL   BMC Syst Biol. 2017 Dec 8;11(1):123. doi: 10.1186/s12918-017-0492-3.
  PUBMED    29221460
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Syst 
            Biol."; date: "2017-12-8"; volume: "11(1):123. doi"; pages: " 
            10.1186/s12918-017-0492-3"
COMMENT     Sequence Label: BB1_12_pGPM1
FEATURES             Location/Qualifiers
     source          1..2337
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     40..57
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     41..57
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(673..689)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(673..689)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      737..768
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        769..871
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             872..1687
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCLKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(945..964)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      1711..1738
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1750..2337)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1830..1849)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaagatcctt tgattttcta ccgaagaaag gcccacccgt gtaaaacgac ggccagttta
       61 tctagtcagc ttgattctag ctgatcgtgg accggaaggt gagccagtga gttgattgca
      121 gtccagttac gctggagtct gaggctcgtc ctgaatgata tgcggcctcg aattcgaaga
      181 cgcggagcct tgggttatta gtagtgtccg ttatttttct gtttaggtta cgattttgcc
      241 agattttttg ggaggaggga aacaaaagaa ccagtgctac acgaccttta agtgccatca
      301 ggcatcctgt tttctcgacc tcatctcatc acatccgtca gtctgagctt tcagttctca
      361 gttttcgatt gactcttgcc ctgctgcgcg cacaccatac cctggctccc tctcatgctt
      421 ctggcgttac cccgggaatc gtacatccat gccgcgaatc ccggacagga ctcagacgga
      481 tttcactatt cctagtgggc acaacctcca tatataagga tacttctgcc ccctagctta
      541 agtgcctcta ttttgtcagt aacaactttc aattacacaa acaaaccatg gcgtcttcga
      601 attcgcgcgt gatcttacgg cattatacgt atgatcggtc cacgatcagc tagattatct
      661 agtcagcttg atgtcatagc tgtttcctga ggctcaatac tgaccattta aatcatacct
      721 gacctccata gcagaaagtc aaaagcctcc gaccggaggc ttttgacttg atcggcacgt
      781 aagaggttcc aactttcacc ataatgaaat aagatcacta ccgggcgtat tttttgagtt
      841 atcgagattt tcaggagcta aggaagctaa aatgagccat attcaacggg aaacgtcttg
      901 cttgaagccg cgattaaatt ccaacatgga tgctgattta tatgggtata aatgggctcg
      961 cgataatgtc gggcaatcag gtgcgacaat ctatcgattg tatgggaagc ccgatgcgcc
     1021 agagttgttt ctgaaacatg gcaaaggtag cgttgccaat gatgttacag atgagatggt
     1081 caggctaaac tggctgacgg aatttatgcc tcttccgacc atcaagcatt ttatccgtac
     1141 tcctgatgat gcatggttac tcaccactgc gatcccaggg aaaacagcat tccaggtatt
     1201 agaagaatat cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg
     1261 gttgcattcg attcctgttt gtaattgtcc ttttaacggc gatcgcgtat ttcgtctcgc
     1321 tcaggcgcaa tcacgaatga ataacggttt ggttggtgcg agtgattttg atgacgagcg
     1381 taatggctgg cctgttgaac aagtctggaa agaaatgcat aaactcttgc cattctcacc
     1441 ggattcagtc gtcactcatg gtgatttctc acttgataac cttatttttg acgaggggaa
     1501 attaataggt tgtattgatg ttggacgagt cggaatcgca gaccgatacc aggatcttgc
     1561 catcctatgg aactgcctcg gtgagttttc tccttcatta cagaaacggc tttttcaaaa
     1621 atatggtatt gataatcctg atatgaataa attgcagttt cacttgatgc tcgatgagtt
     1681 tttctaatga ggacctaaat gtaatcacct ggctcacctt cgggtgggcc tttctgcgtt
     1741 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gatgctcaag
     1801 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     1861 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     1921 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     1981 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     2041 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     2101 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     2161 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     2221 gccagttacc tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     2281 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaa
//
