LOCUS       sequence-190983-        2932 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BB1 with insert: promoter PRPP1B.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Prielhofer R, Barrero JJ, Steuer S, Gassler T, Zahrl R, Baumann K, 
            Sauer M, Mattanovich D, Gasser B, Marx H
  TITLE     GoldenPiCS: a Golden Gate-derived modular cloning system for applied
            synthetic biology in the yeast Pichia pastoris.
  JOURNAL   BMC Syst Biol. 2017 Dec 8;11(1):123. doi: 10.1186/s12918-017-0492-3.
  PUBMED    29221460
REFERENCE   2  (bases 1 to 2932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Syst 
            Biol."; date: "2017-12-8"; volume: "11(1):123. doi"; pages: " 
            10.1186/s12918-017-0492-3"
COMMENT     Sequence Label: BB1_12_pRPP1B
FEATURES             Location/Qualifiers
     source          1..2932
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     40..57
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     41..57
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1268..1284)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1268..1284)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      1332..1363
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        1364..1466
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1467..2282
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCLKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1540..1559)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      2306..2333
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(2345..2932)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2425..2444)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaagatcctt tgattttcta ccgaagaaag gcccacccgt gtaaaacgac ggccagttta
       61 tctagtcagc ttgattctag ctgatcgtgg accggaaggt gagccagtga gttgattgca
      121 gtccagttac gctggagtct gaggctcgtc ctgaatgata tgcggcctcg aattcgaaga
      181 cgcggagcca cttttgcatt tgacacaaaa tacaaattta aggagggaga tcatcgttac
      241 gaagaattta gggagggcgt gacgttccta tgcaactggt ctaaccttat tccgaaagat
      301 ctattcaatt tattttgtta tctggttaag aatgactctg agttgggtgt tacgaatgtt
      361 gaccaaattt cattgcgaat gaaattagag ggattgacac agctacgcaa tgctttggag
      421 gctaagagac aagtgttaca gaaaacatct ttacctcaat cagctcccct tcctgactct
      481 acggaccaga aggtgattgc aagatcaatc aggaacttca acaatgctcg tatctacaga
      541 catactcaag aaaaactcta tgttgctatg attgctgaac tcaaacacat agagaagggc
      601 ttactgaaag agtatcgctc taaagttgtt tcactaaaga gtatttggaa aaaagatgct
      661 acatttacta attccttact actatcacta ggatttgatt cactggaaaa agtagaggat
      721 tctaattttc aagacactgt catcctgttg tggatcatgc gaacactgaa tagagagtat
      781 tatgataaag aagatcaggc agttttaccc gaatggttac ataacctcaa gaccaagttt
      841 ccgttgtcta ctatcaacgt ggagacagag ccttacaaac ctttatacga acagttattt
      901 cctccactag cagaaagaat tccagaggtc tatgccaaag gagactggag cctagagttc
      961 atggcatatg ctggtgttat tctgagttac atcagctaca caagaggtgc caaacaggag
     1021 tgcatactgg tagaaccaat ccagggtgca acatagtaag tgttgcattc aggatgctcg
     1081 tcagtgcgcg aatttgacct cgacctctcg tgactgcgtt tcttcgttgg agaaaaaaaa
     1141 tcgctagacc ataactagaa catttcatct ataaccatca acatggcgtc ttcgaattcg
     1201 cgcgtgatct tacggcatta tacgtatgat cggtccacga tcagctagat tatctagtca
     1261 gcttgatgtc atagctgttt cctgaggctc aatactgacc atttaaatca tacctgacct
     1321 ccatagcaga aagtcaaaag cctccgaccg gaggcttttg acttgatcgg cacgtaagag
     1381 gttccaactt tcaccataat gaaataagat cactaccggg cgtatttttt gagttatcga
     1441 gattttcagg agctaaggaa gctaaaatga gccatattca acgggaaacg tcttgcttga
     1501 agccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata
     1561 atgtcgggca atcaggtgcg acaatctatc gattgtatgg gaagcccgat gcgccagagt
     1621 tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcaggc
     1681 taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg
     1741 atgatgcatg gttactcacc actgcgatcc cagggaaaac agcattccag gtattagaag
     1801 aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc
     1861 attcgattcc tgtttgtaat tgtcctttta acggcgatcg cgtatttcgt ctcgctcagg
     1921 cgcaatcacg aatgaataac ggtttggttg gtgcgagtga ttttgatgac gagcgtaatg
     1981 gctggcctgt tgaacaagtc tggaaagaaa tgcataaact cttgccattc tcaccggatt
     2041 cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa
     2101 taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc
     2161 tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg
     2221 gtattgataa tcctgatatg aataaattgc agtttcactt gatgctcgat gagtttttct
     2281 aatgaggacc taaatgtaat cacctggctc accttcgggt gggcctttct gcgttgctgg
     2341 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgatgc tcaagtcaga
     2401 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     2461 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     2521 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     2581 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     2641 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     2701 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     2761 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     2821 ttacctcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     2881 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aa
//
