LOCUS       sequence-191012-        3606 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CMYA5 (NM_153610, nt 11648 to 12279) in pBSSK+ (minimum binding 
            region to titin).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3606)
  TITLE     Shiang Lab CMYA5 project plasmids (unpublished)
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3606)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: CMYA5tnC/pBSSK+
FEATURES             Location/Qualifiers
     source          1..3606
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     123..142
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     376..393
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    510..531
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(546..551,552..569,570..576)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    584..600
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     589..611
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     608..624
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     608..624
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     643..663
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        645..663
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     700..716
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     700..716
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(1395..1411)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1396..1412)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(1436..1455)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1437..1455)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1465..1482)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1465..1481)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1474..1496)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1623..2078
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1710..1729)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1920..1941
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2104..2208
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2209..2277,2278..3069)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2427..2446)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(3240..3606,1..222)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
       61 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
      121 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
      181 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
      241 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
      301 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
      361 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
      421 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
      481 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
      541 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
      601 tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa
      661 agggaacaaa agctggagct ccaccgcggt ggcggccgct ctagaactag tggatccccc
      721 gggctgcagg aattcgattc catgggactc cagctgaaag ttaacctcca acccaatgat
      781 aactactttt tctatgtgag ggccatcaat gcatttggga caagtgaaca gagtgaagct
      841 gctctcatct ccaccagagg aaccagattt ctcttgttga gagaaacagc tcatcctgct
      901 ctacacattt cctcaagtgg gacagtgatc agctttggtg agaggagacg gctgacggaa
      961 atcccgtcag tgctgggtga ggagctgcct tcctgtggcc agcattactg ggaaaccaca
     1021 gtcacagact gcccagcata tcgactcggc atctgctcca gctcggctgt gcaggcaggt
     1081 gccctaggac aaggggagac ctcatggtac atgcactgct ctgagccaca gagatacaca
     1141 tttttctaca gtggtattgt gagtgatgtt catgtgactg agcgtccagc cagagtgggc
     1201 atcctgctgg actacaacaa ccagagactt atcttcatca acgcagagag cgagcagttg
     1261 ctcttcatca tcaggcacag gtttaatgag ggtgtccacc ctgcctttgc cctggagaaa
     1321 cctggaaaat gtactttgca cctggggata gagcccccgg attctgtaag gcacaaggaa
     1381 ttcatcaagc ttatcgatac cgtcgacctc gagggggggc ccggtaccca attcgcccta
     1441 tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa
     1501 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
     1561 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     1621 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     1681 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     1741 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     1801 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     1861 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     1921 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     1981 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     2041 taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat
     2101 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     2161 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     2221 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     2281 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     2341 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     2401 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     2461 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     2521 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     2581 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     2641 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     2701 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     2761 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     2821 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     2881 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     2941 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     3001 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     3061 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     3121 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     3181 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3241 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3301 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3361 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3421 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3481 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3541 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3601 tacacc
//
