LOCUS       sequence-191033-       11984 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses TET1 with a mutated catalytic domain in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11984)
  AUTHORS   Jin C, Lu Y, Jelinek J, Liang S, Estecio MR, Barton MC, Issa JP
  TITLE     TET1 is a maintenance DNA demethylase that prevents methylation 
            spreading in differentiated cells.
  JOURNAL   Nucleic Acids Res. 2014 Jun;42(11):6956-71. doi: 10.1093/nar/gku372.
            Epub 2014 May 29.
  PUBMED    24875481
REFERENCE   2  (bases 1 to 11984)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gku372"; journalName: "Nucleic Acids Res"; date: 
            "2014-06"; volume: "42"; issue: "11"; pages: "6956-71"
COMMENT     Sequence Label: pIRES-hrGFP II-mTET1
FEATURES             Location/Qualifiers
     source          1..11984
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      53..641
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     542..561
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        786..1165
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1166..1369
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1414..1433
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1414..1432
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             7976..8047
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
     misc_feature    8083..8656
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8251..8268)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8478..8497
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             8662..9381
                     /codon_start=1
                     /product="humanized Renilla green fluorescent protein"
                     /label=hrGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKQILKNTGLQEIMSFKVNLEGVVNNHVFTMEGCGKGNILFGN
                     QLVQIRVTKGAPLPFAFDILSPAFQYGNRTFTKYPEDISDFFIQSFPAGFVYERTLRYE
                     DGGLVEIRSDINLIEGMFVYRVEYKGRNFPNDGPVMKKTITGLQPSFEVVYMNDGVLVG
                     QVILVYRLNSGKFYSCHMRTLMKSKGVVKDFPEYHFIQHRLEKTYVEDGGFVEQHETAI
                     AQLTSLGKPLGSLHEWV"
     primer_bind     complement(9412..9432)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(9412..9430)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9450..9467)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9456..9567
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(9676..10131)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9813..9834)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10025..10044
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        10158..10262
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        10264..10455
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(10287..10307)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      10452..10586
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             10635..11429
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             10635..11429
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(10689..10708)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     11299..11318
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(11617..11636)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    11661..11708
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
       61 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
      121 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
      181 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
      241 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
      301 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
      361 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
      421 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
      481 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
      541 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
      601 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
      661 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
      721 ctgattctgt ggataaccgt attaccgcca ggcgcgccgc gatgtacggg ccagatttac
      781 gcgttgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca
      841 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
      901 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
      961 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
     1021 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
     1081 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
     1141 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg
     1201 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
     1261 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgaa
     1321 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc tctggctaac
     1381 tagagaaccc actgcttact ggcttatcga aattaatacg actcactata gggagaccca
     1441 agcttctgga ggcccgggct ttcagggtac cgaagaagga tccaaggagg aattcgccct
     1501 tattatggac tctgtagcta tgtctcgatc ccgccatgca aggccttcca gattagtcag
     1561 gaaggaagat gtaaacaaaa aaaagaaaaa cagccaacta cgaaagacaa ccaagggagc
     1621 caacaaaaat gtggcatcag tcaagacttt aagccctgga aaattaaagc aattaattca
     1681 agaaagagat gttaagaaaa aaacagaacc taaaccaccc gtgccagtca gaagccttct
     1741 gacaagagct ggagcagcac gcatgaattt ggataggact gaggttcttt ttcagaaccc
     1801 agagtcctta acctgcaatg ggtttacaat ggcgctacga agcacctctc ttagcaggcg
     1861 actctcccaa cccccactgg tcgtagccaa atccaaaaag gttccacttt ctaagggttt
     1921 agaaaagcaa catgattgtg attataagat actccctgct ttgggagtaa agcactcaga
     1981 aaatgattcg gttccaatgc aagacaccca agtccttcct gatatagaga ctctaattgg
     2041 tgtacaaaat ccctctttac ttaaaggtaa gagccaagag acaactcagt tttggtccca
     2101 aagagttgag gattccaaga tcaatatccc tacccacagt ggccctgcag ctgagatcct
     2161 tcctgggcca ctggaaggga cacgctgtgg tgaaggacta ttctctgaag agacattgaa
     2221 tgataccagt ggttccccaa aaatgtttgc tcaggacaca gtgtgtgctc cttttcccca
     2281 aagagcaacc cccaaagtta cctctcaagg aaaccccagc attcagttag aagagttggg
     2341 ttcacgagta gaatctctta agttatctga ttcttacctg gatcccatta aaagtgaaca
     2401 tgattgctac cccacctcca gtcttaataa ggttatacct gacttgaacc ttagaaactg
     2461 cttggctctt ggtgggtcta cgtctcctac ctctgtaata aaattcctct tggcaggctc
     2521 aaaacaagcg acccttggtg ctaaaccaga tcatcaagag gccttcgaag ctactgcaaa
     2581 tcaacaggaa gtttctgata ccacctcttt cctaggacag gcctttggtg ctatcccaca
     2641 tcaatgggaa cttcctggtg ctgacccagt tcatggtgag gccctgggtg agaccccaga
     2701 tctaccagag attcctggtg ctattccagt ccaaggagag gtctttggta ctattttaga
     2761 ccaacaagaa actcttggta tgagtgggag tgttgtccca gacttgcctg tcttccttcc
     2821 tgttcctcca aatccaattg ctacctttaa tgctccttcc aaatggcctg agccccaaag
     2881 cactgtctca tatggacttg cagtccaggg tgctatacag attttgcctt tgggctcagg
     2941 acacactcct caatcatcat caaactcaga gaaaaattca ttacctccag taatggctat
     3001 aagcaatgta gaaaatgaga agcaggttca tataagcttc ctgccagcta acactcaggg
     3061 gttcccatta gcccctgaga gaggactctt ccatgcttca ctgggtatag cccaactctc
     3121 tcaggctggt cctagcaaat cagacagagg gagctcccag gtcagtgtaa ccagcacagt
     3181 tcatgttgtc aacaccacag tggtgactat gccagtgcca atggtcagta cctcctcttc
     3241 ttcctatacc actttgctac cgactttgga aaagaagaaa agaaagcgat gtggggtctg
     3301 tgaaccctgc cagcagaaga ccaactgtgg tgaatgcact tactgcaaga acagaaagaa
     3361 cagccatcag atctgtaaga aaagaaaatg tgaggagctg aaaaagaaac catctgttgt
     3421 tgtgcctctg gaggttataa aggaaaacaa gaggccccag agggaaaaga agcccaaagt
     3481 tttaaaggca gattttgaca acaaaccagt aaatggcccc aagtcagaat ccatggacta
     3541 cagtagatgt ggtcatgggg aagaacaaaa attggaattg aacccacata ctgttgaaaa
     3601 tgtaactaaa aatgaagaca gcatgacagg catcgaggtg gagaagtgga cacaaaacaa
     3661 gaaatcacag ttaactgatc acgtgaaagg agattttagt gctaatgtcc cagaagctga
     3721 aaaatcgaaa aactctgaag ttgacaagaa acgaaccaaa tctccaaaat tgtttgtaca
     3781 aaccgtaaga aatggcatta aacatgtaca ctgtttacca gctgaaacaa atgtttcatt
     3841 taaaaaattc aatattgaag aattcggcaa gacattggaa aacaattctt ataaattcct
     3901 aaaagacact gcaaaccata aaaacgctat gagctctgtt gctactgata tgagttgtga
     3961 tcatctcaag gggagaagta acgttttagt attccagcaa cctggcttta actgcagttc
     4021 cattccacat tcttcacact ccatcataaa tcatcatgct agtatacaca atgaaggtga
     4081 tcaaccaaaa actcctgaga atataccaag taaagaacca aaagatggat ctcccgttca
     4141 accaagtctc ttatcgttaa tgaaagatag gagattaaca ttggagcaag tggtagccat
     4201 agaggccctg actcaactct cagaagcccc atcagagaat tcctccccat caaagtcaga
     4261 gaaggatgag gaatcagagc agagaacagc cagtttgctt aatagctgca aagctatcct
     4321 ctacactgta agaaaagacc tccaagaccc aaacttacag ggagagccac caaaacttaa
     4381 tcactgtcca tctttggaaa aacaaagttc atgcaacacg gtggttttca atgggcaaac
     4441 tactaccctt tccaactcac atatcaactc agctactaac caagcatcca caaagtcaca
     4501 tgaatattca aaagtcacaa attcattatc tctttttata ccaaaatcaa attcatccaa
     4561 gattgacacc aataaaagta ttgctcaagg gataattact cttgacaatt gttccaatga
     4621 tttgcatcag ttgccaccaa gaaataatga agtggagtat tgcaaccagt tactggacag
     4681 cagcaaaaaa ttggactcag atgatctatc atgtcaggat gcaacccata cccaaattga
     4741 ggaagatgtt gcaacacagt tgacacaact tgcttcgata attaagatca attatataaa
     4801 accagaggac aaaaaagttg aaagtacacc aacaagcctt gtcacatgta atgtacagca
     4861 aaaatacaat caggagaagg gcacaatgca acagaaacca ccttcaagtg tacacaataa
     4921 tcatggttca tcattaacaa aacaaaagaa cccaacccag aaaaagacaa aatccacccc
     4981 atcaagagat cggcggaaaa agaagcccac agttgtaagt tatcaagaaa atgatcggca
     5041 gaagtgggaa aagttgtcct atatgtatgg cacaatatgc gacatttgga tagcatcgaa
     5101 atttcaaaat tttgggcaat tttgtccaca tgattttcct actgtatttg ggaaaatttc
     5161 ttcctcgacc aaaatatgga aaccactggc tcaaacgagg tccattatgc aacccaaaac
     5221 agtatttcca ccactcactc agataaaatt acagagatat cctgaatcag cagaggaaaa
     5281 ggtgaaggtt gaaccattgg attcactcag cttatttcat cttaaaacgg aatccaacgg
     5341 gaaggcattc actgataaag cttataattc tcaggtacag ttaacggtga atgccaatca
     5401 gaaagcccat cctttgaccc agccctcctc tccacctaac cagtgtgcta acgtgatggc
     5461 aggcgatgac caaatacggt ttcagcaggt tgttaaggag caactcatgc atcagagact
     5521 gccaacattg cctggtatct ctcatgaaac acccttaccg gagtcagcac taactctcag
     5581 gaatgtaaat gtagtgtgtt caggtggaat tacagtggtt tctaccaaaa gtgaagagga
     5641 agtctgttca tccagttttg gaacatcaga attttccaca gtggacagtg cacagaaaaa
     5701 ttttaatgat tatgccatga acttctttac taaccctaca aaaaacctag tgtctataac
     5761 taaagattct gaactgccca cctgcagctg tcttgatcga gttatacaaa aagacaaagg
     5821 cccatattat acacaccttg gggcaggacc aagtgttgct gctgtcaggg aaatcatgga
     5881 gaataggtat ggtcaaaaag gaaacgcaat aaggatagaa atagtagtgt acaccggtaa
     5941 agaagggaaa agctctcatg ggtgtccaat tgctaagtgg gttttaagaa gaagcagtga
     6001 tgaagaaaaa gttctttgtt tggtccggca gcgtacaggc caccactgtc caactgctgt
     6061 gatggtggtg ctcatcatgg tgtgggatgg catccctctt ccaatggccg accggctata
     6121 cacagagctc acagagaatc taaagtcata caatgggcac cctaccgaca gaagatgcac
     6181 cctcaatgaa aatcgtacct gtacatgtca aggaattgat ccagagactt gtggagcttc
     6241 attctctttt ggctgttcat ggagtatgta ctttaatggc tgtaagtttg gtagaagccc
     6301 aagccccaga agatttagaa ttgatccaag ctctccctta catgaaaaaa accttgaaga
     6361 taacttacag agtttggcta cacgattagc tccaatttat aagcagtatg ctccagtagc
     6421 ttaccaaaat caggtggaat atgaaaatgt tgcccgagaa tgtcggcttg gcagcaagga
     6481 aggtcgtccc ttctctgggg tcactgcttg cctggacttc tgtgctcatc cctacagggc
     6541 cattcacaac atgaataatg gaagcactgt ggtttgtacc ttaactcgag aagataaccg
     6601 ctctttgggt gttattcctc aagatgagca gctccatgtg ctacctcttt ataagctttc
     6661 agacacagat gagtttggct ccaaggaagg aatggaagcc aagatcaaat ctggggccat
     6721 cgaggtcctg gcaccccgcc gcaaaaaaag aacgtgtttc actcagcctg ttccccgttc
     6781 tggaaagaag agggctgcga tgatgacgga ggttcttgca cataagataa gggcagtgga
     6841 aaagaaacct attccccgaa tcaagcggaa gaataactca acaacaacaa acaacagtaa
     6901 gccttcgtca ctgccaacct tagggagtaa cactgagacc gtgcaacctg aagtaaaaag
     6961 tgaaaccgaa ccccatttta tcttaaaaag ttcagacaac actaaaactt attcgctgat
     7021 gccatccgct cctcacccag tgaaagaggc atctccaggc ttctcctggt ccccgaagac
     7081 tgcttcagcc acaccagctc cactgaagaa tgacgcaaca gcctcatgcg ggttttcaga
     7141 aagaagcagc actccccact gtacgatgcc ttcgggaaga ctcagtggtg ccaatgcagc
     7201 tgctgctgat ggccctggca tttcacagct tggcgaagtg gctcctctcc ccaccctgtc
     7261 tgctcctgtg atggagcccc tcattaattc tgagccttcc actggtgtga ctgagccgct
     7321 aacgcctcat cagccaaacc accagccctc cttcctcacc tctcctcaag accttgcctc
     7381 ttctccaatg gaagaagatg agcagcattc tgaagcagat gagcctccat cagacgaacc
     7441 cctatctgat gaccccctgt cacctgctga ggagaaattg ccccacattg atgagtattg
     7501 gtcagacagt gagcacatct ttttggatgc aaatattggt ggggtggcca tcgcacctgc
     7561 tcacggctcg gttttgattg agtgtgcccg gcgggagctg cacgctacca ctcctgttga
     7621 gcaccccaac cgtaatcatc caacccgcct ctcccttgtc ttttaccagc acaaaaacct
     7681 aaataagccc caacatggtt ttgaactaaa caagattaag tttgaggcta aagaagctaa
     7741 gaataagaaa atgaaggcct cagagcaaaa agaccaggca gctaatgaag gtccagaaca
     7801 gtcctctgaa gtaaatgaat tgaaccaaat tccttctcat aaagcattaa cattaaccca
     7861 tgacaatgtt gtcaccgtgt ccccttatgc tctcacacac gttgcggggc cctataacca
     7921 ttgggtctgt aggataaggg cgaattctgc agatatccat cacactggcg gccgcgacta
     7981 caaggatgac gatgacaagg attacaaaga cgacgatgat aaggactata aggatgatga
     8041 cgacaaataa tagcaattcc tcgacgactg catagggtta cccccctctc cctccccccc
     8101 cccntaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt
     8161 tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct
     8221 tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga
     8281 atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga
     8341 ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac
     8401 gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag
     8461 ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc
     8521 agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg
     8581 tttagtcgag gttaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg
     8641 aaaaacacga tgataatgac catggtgagc aagcagatcc tgaagaacac cggcctgcag
     8701 gagatcatga gcttcaaggt gaacctggag ggcgtggtga acaaccacgt gttcaccatg
     8761 gagggctgcg gcaagggcaa catcctgttc ggcaaccagc tggtgcagat ccgcgtgacc
     8821 aagggcgccc ccctgccctt cgccttcgac atcctgagcc ccgccttcca gtacggcaac
     8881 cgcaccttca ccaagtaccc cgaggacatc agcgacttct tcatccagag cttccccgcc
     8941 ggcttcgtgt acgagcgcac cctgcgctac gaggacggcg gcctggtgga gatccgcagc
     9001 gacatcaacc tgatcgaggg gatgttcgtg taccgcgtgg agtacaaggg ccgcaacttc
     9061 cccaacgacg gccccgtgat gaagaagacc atcaccggcc tgcagcccag cttcgaggtg
     9121 gtgtacatga acgacggcgt gctggtgggc caggtgatcc tggtgtaccg cctgaacagc
     9181 ggcaagttct acagctgcca catgcgcacc ctgatgaaga gcaagggcgt ggtgaaggac
     9241 ttccccgagt accacttcat ccagcaccgc ctggagaaga cctacgtgga ggacggcggc
     9301 ttcgtagagc agcacgagac cgccatcgcc cagctgacca gcctgggcaa gcccctgggc
     9361 agcctgcacg agtgggtgta agctcgagca tgcatctaga gggccctatt ccctttagtg
     9421 agggttaatt gctagagctc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
     9481 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc tcactgtcct
     9541 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     9601 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
     9661 ggcgccggcg cgcgtaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt
     9721 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa
     9781 aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa
     9841 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac
     9901 gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga
     9961 accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa
    10021 aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc
    10081 tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg tcaggtggca
    10141 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
    10201 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
    10261 atcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
    10321 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
    10381 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
    10441 agcaaccata gtcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
    10501 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
    10561 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa gatcgatcaa gagacaggat
    10621 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
    10681 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
    10741 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
    10801 ccctgaatga actgcaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
    10861 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
    10921 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca
    10981 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
    11041 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
    11101 atgatctgga cgaagaacat caggggctcg cgccagccga actgttcgcc aggctcaagg
    11161 cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata
    11221 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
    11281 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagaactt ggcggcgaat
    11341 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
    11401 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca
    11461 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt
    11521 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
    11581 gctggagttc ttcgcccacc ctagggggag gctaactgaa acacggaagg agacaatacc
    11641 ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg gtgttgggtc
    11701 gtttgttcat aaacgcgggg ttcggtccca gggctggcac tctgtcgata ccccaccgag
    11761 accccattgg ggccaatacg cccgcgtttc ttccttttcc ccaccccacc ccccaagttc
    11821 gggtgaaggc ccagggctcg cagccaacgt cggggcggca ggccctgcca tagcctcagg
    11881 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
    11941 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtg
//
