LOCUS       sequence-19106-e         693 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid was designed for screening purposes and may contain 
            discrepancies relative to the current canonical GenBank version of 
            the gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, 
            Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW
  TITLE     Identification of poxvirus CD8+ T cell determinants to enable 
            rational design and characterization of smallpox vaccines.
  JOURNAL   J Exp Med. 2005 January 3; 201(1): 95–104.
  PUBMED    15623576
REFERENCE   2  (bases 1 to 693)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "2005-?-3"; volume: " 201(1)"; pages: " 95–104"
COMMENT     Sequence Label: DCT.VV.Orf22
FEATURES             Location/Qualifiers
     source          1..693
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      176..185
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             358..399
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             409..426
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(449..466)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    455..681
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ctcggatcca ctagtccagt gtggtggaat tgtcttgacc cttcaccgaa ttccagctga
       61 ccaccatgat aacatccatt gttaatcttt gtcaaatatt gctcgtccaa cgagtaacat
      121 ccgtctggag atatcccgtt agaaatataa aaccaactaa tattgagaaa ttcatccatg
      181 gtggcatttt gtatgctgcg tttctttggc tcttctatca accacatatc tgcgacggag
      241 cattttctat ctttaatatc taggcaattc ccggggatcc gaataaaggg tcaagacaat
      301 tctgcagata tccagcacag tggcggccgc tcgagtctag agggcccgcg gttcgaaggt
      361 aagcctatcc ctaaccctct cctcggtctc gattctacgc gtaccggtca tcatcaccat
      421 caccattgag tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
      481 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
      541 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
      601 ggtggggtgt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
      661 gggatgcggt gggctctatg gcttctgagg cgg
//
