LOCUS       sequence-191063-        6021 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Centrosomes, Excitation: 505 / 569, Emission: 516 / 
            581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6021)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 6021)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdEos-Gamma-Tubulin-17
FEATURES             Location/Qualifiers
     source          1..6021
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             1947..2627
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="MSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     polyA_signal    2749..2870
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2786..2805)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2840..2859
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2877..3332)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3014..3035)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3226..3245
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3359..3463
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3465..3822
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3465..3656
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3488..3508)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3673..3808
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3857..4651
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3857..4651
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3911..3930)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4521..4540
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4839..4858)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4883..4930
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5259..5847
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5748..5767
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcca
      541 ccatgccgag ggaaatcatc accctacagt tgggccagtg cggcaatcag attgggttcg
      601 agttctggaa acagctgtgc gccgagcatg gtatcagccc cgagggcatc gtggaggagt
      661 tcgccaccga gggcactgac cacaaggacg tctttttcta ccaggcagac gatgagcact
      721 acatcccccg ggccgtgctg ctggacttgg aaccccgggt gatccactcc atcctcaact
      781 ccccctatgc caagctctac aacccagaga acatctacct gtcggaacat ggaggaggag
      841 ctggcaacaa ctgggccagc ggattctccc agggagaaaa gatccatgag gacatttttg
      901 acatcataga ccgggaggca gatggtagtg acagtctaga gggctttgtg ctgtgtcact
      961 ccattgctgg ggggacaggc tctggactgg gttcctacct cttagaacgg ctgaatgaca
     1021 ggtatcctaa gaagctggtg cagacatact cagtgtttcc caaccaggac gagatgagcg
     1081 atgtggtggt ccagccttac aattcactcc tcacactcaa gaggctgacg cagaatgcag
     1141 actgtgtggt ggtgctggac aacacagccc tgaaccggat tgccacagac cgcctgcaca
     1201 tccagaaccc atccttctcc cagatcaacc agctggtgtc taccatcatg tcagccagca
     1261 ccaccaccct gcgctaccct ggctacatga acaatgacct catcggcctc atcgcctcgc
     1321 tcattcccac cccacggctc cacttcctca tgaccggcta cacccctctc actacggacc
     1381 agtcagtggc cagcgtgagg aagaccacgg tcctggatgt catgaggcgg ctgctgcagc
     1441 ccaagaacgt gatggtgtcc acaggccgag accgccagac caaccactgc tacatcgcca
     1501 tcctcaacat catccaggga gaggtggacc ccacccaggt ccacaagagc ttgcagagga
     1561 tccgggaacg caagttggcc aacttcatcc cgtggggccc cgccagcatc caggtggccc
     1621 tgtcgaggaa gtctccctac ctgccctcgg cccaccgggt cagcgggctc atgatggcca
     1681 accacaccag catctcctcg ctcttcgaga gaacctgtcg ccagtatgac aagctgcgta
     1741 agcgggaggc cttcctggag cagttccgca aggaggacat gttcaaggac aactttgatg
     1801 agatggacac atccagggag attgtgcagc agctcatcga tgagtaccat gcggccacac
     1861 ggccagacta catctcctgg ggcactcagg agcagcgaat tctgcagtcg acggtaccgc
     1921 gggcccggga tccaccggtc gccaccatga gtgcgattaa gccagacatg aagatcaacc
     1981 tccgtatgga aggcaacgta aacgggcacc actttgtgat cgacggagat ggtacaggca
     2041 agccttttga gggaaaacag agtatggatc ttgaagtcaa agagggcgga cctctgcctt
     2101 ttgcctttga tatcctgacc actgcattcc attacggcaa cagggtattc gccgaatatc
     2161 cagaccacat acaagactat tttaagcagt cgtttcctaa ggggtattcg tgggaacgaa
     2221 gcttgacttt cgaagacggg ggcatttgca ttgccagaaa cgacataaca atggaagggg
     2281 acactttcta taataaagtt cgatttcacg gtgtaaactt tcccgccaat ggtccagtta
     2341 tgcagaagaa gacgctgaaa tgggagccct ccactgagaa aatgtatgtg cgtgatggag
     2401 tgctgactgg tgatattcgc atggctttgt tgcttgaagg aaatgcccat taccgatgtg
     2461 acttcagaac tacttacaaa gctaaggaga agggtgtcaa gttaccaggc taccactttg
     2521 tggaccactg cattgagatt ttaagccatg acaaagatta caacaaggtt aagctgtatg
     2581 agcatgctgt tgctcattct ggattgcctg acaatgccag acgataagcg gccgcgactc
     2641 tagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca
     2701 cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt
     2761 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     2821 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta aggcgtaaat
     2881 tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
     2941 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
     3001 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
     3061 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc
     3121 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
     3181 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
     3241 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
     3301 cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg
     3361 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     3421 ataaccctga taaatgcttc aataatattg aaaaaggaag agtcctgagg cggaaagaac
     3481 cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
     3541 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     3601 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
     3661 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     3721 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     3781 aagtagtgag gaggcttttt tggaggccta ggcttttgca aagatcgatc aagagacagg
     3841 atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg
     3901 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
     3961 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
     4021 tgccctgaat gaactgcaag acgaggcagc gcggctatcg tggctggcca cgacgggcgt
     4081 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
     4141 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
     4201 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
     4261 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
     4321 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
     4381 ggcgagcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa
     4441 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
     4501 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
     4561 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
     4621 cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac
     4681 caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg
     4741 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
     4801 atgctggagt tcttcgccca ccctaggggg aggctaactg aaacacggaa ggagacaata
     4861 ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca cggtgttggg
     4921 tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcga taccccaccg
     4981 agaccccatt ggggccaata cgcccgcgtt tcttcctttt ccccacccca ccccccaagt
     5041 tcgggtgaag gcccagggct cgcagccaac gtcggggcgg caggccctgc catagcctca
     5101 ggttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     5161 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     5221 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     5281 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     5341 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     5401 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     5461 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     5521 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     5581 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     5641 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     5701 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     5761 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     5821 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     5881 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     5941 aaccgtatta ccgccatgca ttagttatta atagtaatca attacggggt cattagttca
     6001 tagcccatat atggagttcc g
//
