LOCUS       sequence-191067-       12808 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Talin/Focal Adhesions, C1 Cloning Vector, 
            Excitation:487, Emission:509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12808)
  TITLE     Michael Davidson Unpublished
REFERENCE   2  (bases 1 to 12808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Talin-C-18
FEATURES             Location/Qualifiers
     source          1..12808
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      547..556
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(553..555,556..558,559..1269)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(598..619)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(859..878)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1206..1227
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     regulatory      1318..1327
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    9536..9657
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9573..9592)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9627..9646
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(9664..10119)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9801..9822)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10013..10032
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        10146..10250
                     /gene="bla"
                     /label=AmpR promoter
     promoter        10252..10609
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        10252..10443
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(10275..10295)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      10460..10595
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             10644..11438
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             10644..11438
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(10698..10717)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     11308..11327
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(11626..11645)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    11670..11717
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      12046..12634
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12535..12554
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      601 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      661 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      721 ccctggccca ccctcgtgac caccttgacc tacggcgtgc agtgcttcgc ccgctacccc
      781 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      841 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      901 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
      961 atcctggggc acaagctgga gtacaactac aacagccaca aggtctatat caccgccgac
     1021 aagcagaaga acggcatcaa ggtgaacttc aagacccgcc acaacatcga ggacggcagc
     1081 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1141 cccgacaacc actacctgag cacccagtcc aagctgagca aagaccccaa cgagaagcgc
     1201 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1261 ctgtacaagt ccggactcag atctcgagct caagctagct tcgaattcgc agaagctgcc
     1321 accatggttg cgctttcgct gaagattagc attgggaatg tggtgaagac gatgcaattt
     1381 gagccatcta ccatggtgta cgacgcctgc cgcatgattc gtgagcggat cccagaggcc
     1441 ctggctggcc ctcccaacga ctttgggctc tttctgtcag atgatgaccc caaaaaaggc
     1501 atctggctag aggctgggaa agctttggac tactacatgc tccgaaatgg ggacaccatg
     1561 gagtacagaa aaaagcagag acccctgaag atacggatgt tagacggaac ggtgaagact
     1621 atcatggtgg atgactccaa gaccgtcaca gacatgctca tgaccatttg tgcccgaatt
     1681 ggtatcacca accatgatga gtattcactg gttcgagagc tgatggaaga aaagaaagat
     1741 gaggggacag ggaccctgag aaaagacaag accctactgc gagatgaaaa gaagatggag
     1801 aagctaaagc agaagttgca cacagacgat gagttgaact ggctggacca tgggcggaca
     1861 ctgagggaac agggagtgga agagcatgag acgttgctgc tgcggagaaa gttcttctac
     1921 tcagaccaga atgtggattc ccgagaccct gtacagctga accttctcta tgtgcaggca
     1981 cgagatgaca tcctgaatgg ctcccatcct gtctcctttg acaaggcctg tgaatttgca
     2041 ggcttccagt gccagatcca gtttggacct cacaatgaac agaagcacaa ggctggcttc
     2101 cttgacctga aggacttcct gcccaaggag tatgtgaagc agaagggaga gcgtaagatc
     2161 tttcaggcac acaagaattg tgggcagatg agtgaaattg aggccaaggt acgctacgtg
     2221 aagctggccc gttccctcaa gacttacggt gtctccttct tcctagtcaa ggaaaagatg
     2281 aaggggaaga ataaactggt gcccaggctg ttgggcatca ctaaggagtg tgtgatgcgt
     2341 gtggatgaga agaccaagga ggtgattcag gaatggagcc tcaccaacat caaacgctgg
     2401 gctgcctctc ccaagagctt tactctggac tttggagact atcaggatgg ctactactca
     2461 gtacagacga cagaaggcga gcagatcgca cagctcattg ctggctacat agatatcatc
     2521 cttaagaaga aaaaaagcaa ggaccatttt gggctggagg gagatgaaga gtctactatg
     2581 ttggaggact cagtgtctcc caaaaagtca acagtccttc agcagcagta caaccgagtg
     2641 gggaaagtgg agcacggctc tgtggctctg ccagccatca tgcgctctgg agcctctggt
     2701 cctgagaatt tccaggtggg gagcatgcca cctgcccagc agcagatcac cagtggccag
     2761 atgcaccgag gacacatgcc acctctgact tcagcccagc aggcgctcac tggaaccatt
     2821 aactccagca tgcaggctgt gcaggctgcc caggccactc tggatgactt tgagactcta
     2881 ccccctcttg gccaggatgc tgcctccaaa gcttggcgta agaacaagat ggatgaatca
     2941 aagcatgaga tccactccca ggtagatgcc atcacagctg gcactgcctc tgtggtaaac
     3001 ctgacagcag gagatcctgc agagacagac tatacagcag tgggctgtgc agtcaccacc
     3061 atctcttcca acctgacgga gatgtcccgt ggggtgaagc tattggctgc cctgctggag
     3121 gatgaaggcg gcaatggccg gccccttctg caagcagcaa agggccttgc aggggctgtg
     3181 tcggaactgc ttcgcagtgc tcagcccgcc agcgctgagc ctcgtcagaa cctgctgcag
     3241 gcagccggga acgtgggcca ggccagtggg gagctgttgc agcaaattgg ggaaagtgac
     3301 actgaccccc acttccagga tgttctaatg cagctagcaa acgcagtggc aagtgctgca
     3361 gccgccctgg tcctcaaggc caagagtgtg gcccagcgca cagaggattc cgggcttcag
     3421 acccaagtga ttgctgcagc tacgcaatgt gctctgtcca cttcccagct ggtggcctgt
     3481 accaaggtgg tagcacctac aatcagctca cctgtgtgcc aagaacagct agtcgaggct
     3541 gggcgactgg tggccaaagc tgtggagggc tgtgtgtctg cctcgcaagc agctacagaa
     3601 gatggacagc tgcttcgagg ggtaggagca gcagccacgg ctgtcaccca ggccctcaat
     3661 gagctgctgc agcacgtgaa ggcccacgcc accggagctg ggcctgccgg tcggtatgac
     3721 caagccactg ataccatcct caccgtcact gaaaacatct tcagctccat gggtgatgct
     3781 ggggagatgg tgcgccaggc ccgcatcctg gcccaagcca cctcagacct ggtcaatgct
     3841 atcaaggccg atgctgaggg ggagagtgat ctggagaact ctagaaagct cctgagtgct
     3901 gccaagatcc tcgctgatgc caccgccaag atggtggagg cggccaaggg agcagccgcc
     3961 caccctgaca gtgaggaaca gcagcagcga ctgcgtgaag cagctgaggg gcttcgcatg
     4021 gccaccaatg cagctgcgca gaacgccatc aagaagaagt tggtgcagcg cctggagcat
     4081 gcagccaagc aagctgcagc ctctgcaaca cagaccattg ctgcagccca acatgcagcc
     4141 tctgccccca aggcctctgc gggcccccag cccctactgg tgcagagctg taaggccgtg
     4201 gcagagcaga ttccgttgct ggtgcagggt gtccgaggaa gtcaagctca gcctgacagc
     4261 cctagtgctc agctcgccct cattgctgcc agccagagct tcctgcagcc aggtgggaag
     4321 atggtggcag cggcaaaagc ctcagtgcca acaattcagg accaggcttc agcgatgcag
     4381 ctgagtcagt gcgcaaagaa cctaggcact gccctggccg agctgcgcac tgctgctcag
     4441 aaggctcagg aggcatgtgg acctttggag atggattctg cactgagtgt tgtacaaaat
     4501 ctagagaaag acctacagga aataaaagca gcagctcgag atggcaagct taagccctta
     4561 cctggggaaa caatggagaa gtgtacccaa gatcttggca acagcaccaa agcggtgagc
     4621 tctgccatcg ccaagctgct gggggagatt gcccagggca acgagaacta tgcaggtatt
     4681 gcagctcggg atgtagctgg tggactaagg tcactagccc aggctgcgcg gggtgtggct
     4741 gcgctgacat cagatcctgc agtgcaggcc attgtgcttg acacagccag cgacgttctg
     4801 gacaaggcca gcagcctcat tgaagaggca aaaaaagcat ctggacaccc aggggaccca
     4861 gaaagccagc aaaggcttgc tcaggtagct aaagcagtga cccaagccct gaaccgctgt
     4921 gtcagctgcc tgcctggcca aagagacgtg gataatgccc tacgagccgt tggagatgcc
     4981 agcaagcgac tcctgagtga cttgcttcct ccaagcacgg ggacatttca agaagcacag
     5041 agccgattga atgaagctgc agctgggtta aatcaggccg ccacagaact ggtgcaagcc
     5101 tcccgaggaa ctcctcagga cctggctcgg gcctccggcc gatttggaca ggacttcagc
     5161 accttcctgg aagctggtgt ggagatggcc ggacaggccc cgagccagga ggaccgagcc
     5221 caggtggtgt ccaacctgaa gggcatatcc atgtcttcaa gcaaacttct ccttgccgct
     5281 aaggccttgt ccacagaccc tgcttctccc aacctcaaga gtcagctggc tgcggctgcc
     5341 cgggcagtga cggacagcat caaccagctc atcaccatgt gcacccagca ggcacctggc
     5401 cagaaggagt gtgacaatgc acttcggcag ctagagacag tccgagaact cctggagaat
     5461 ccagtccagc ccatcaacga catgtcctac ttcggttgct tggacagtgt catggagaac
     5521 tccaaggtcc taggtgaggc catgactggc atctcccaaa atgccaagaa tggaaatctg
     5581 ccggagtttg gagatgccat tgccacagcc tccaaggctc tctgtggctt cacggaggca
     5641 gccgcacagg cagcatatct agttggtgtc tctgacccca acagccaagc tggacagcaa
     5701 ggactggtgg aacccacaca gtttgcccgt gcaaaccagg caattcagat ggcctgtcag
     5761 agtctggggg agcctggctg tacccaggcc caggtgttat ctgcagccac tattgtagcc
     5821 aaacacacat ctgcattgtg taacagctgt cgcctggctt ccgctaggac tgccaatccc
     5881 actgccaagc gccagtttgt acagtcagcc aaggaggtgg ccaacagtac agccaatctt
     5941 gtcaagacca tcaaggcact agatggggac ttcacagaag agaaccgtgc ccagtgccga
     6001 gcagccacag cccctctgct ggaagctgtg gataacctga gtgcctttgc ctccaaccct
     6061 gagttctcca gcgtccctgc ccagatcagc cctgagggca gagcagccat ggagcccatt
     6121 gtaatctctg ctaagacaat gttggagagt gctggagggc tcatccagac agctcgggcg
     6181 ttagcagtca atccccgaga ccccccacgt tggtctgtgc tagctggcca ctctcggact
     6241 gtctcagatt ccatcaagaa acttattaca agcatgaggg acaaagcccc agggcagctg
     6301 gagtgtgaga cagccattgc ggctctgaac agctgcttgc gggacctaga tcaggcttcg
     6361 cttgccgctg tcagccagca gcttgctccc cgtgaaggaa tctctcaaga ggctttgcac
     6421 acccagatgc tcactgcagt gcaagaaatt tctcatctca ttgagccgct ggccagcgct
     6481 gctcgggctg aagcctccca gctgggacac aaggtgtccc aaatggccca gtactttgaa
     6541 ccactcaccc tggctgcagt gggtgctgcg tctaagaccc tgagccaccc gcagcagatg
     6601 gcacttctgg accagactaa aacgttggca gagtctgcct tgcagttgct atatactgcc
     6661 aaggaggctg gtggtaaccc caagcaagca gcacacaccc aggaagccct ggaggaggct
     6721 gtgcagatga tgacagaggc cgtagaagac ctgacgacga ccctcaacga agcagccagt
     6781 gctgcaggag tcgttggcgg catggtggac tctatcactc aggccatcaa ccagctagat
     6841 gaaggaccta tgggtgaccc agaaggctca ttcgtagatt accagacaac catggtgagg
     6901 acagccaagg ccattgctgt cactgttcag gagatggtaa ccaagtcaaa caccagccct
     6961 gaagagctag gccctcttgc caaccagctg accagtgact atggccgact ggcctcacaa
     7021 gccaagcctg cagctgtggc tgctgaaaat gaagagatag gcgctcacat caaacaccga
     7081 gtacaggagc tgggccatgg ctgctctgct ctggtcacca aggcaggtgc cttgcagtgt
     7141 agccccagtg atgtctacac caagaaggag ctcatagagt gtgcccgcag agtgtcagag
     7201 aaggtctccc atgttctggc tgcactccag gctgggaatc gtggtaccca ggcctgcatt
     7261 acagcagcca gtgctgtgtc tggtatcatt gctgacctcg acaccaccat catgttcgct
     7321 actgctggca cacttaaccg tgagggtgct gaaacttttg ctgaccaccg ggagggtatc
     7381 ttaaagacag caaaggttct tgtggaggac accaaggtcc tagtgcagaa tgcagctggg
     7441 agccaggaga agttggcaca agccgcccag tcctccgtgg ccaccattac ccgcctcgct
     7501 gatgtggtca agctcggtgc agccagccta ggagccgaag accctgaaac tcaggtggtg
     7561 ctgatcaatg cagtaaagga cgtagccaag gccctgggtg acctcatcag cgctacgaag
     7621 gctgcagcgg gcaaagttgg ggatgaccct gcagtgtggc agctcaagaa ctctgccaag
     7681 gtgatggtga ccaatgtgac atcattgctc aagacagtga aggctgtgga agatgaggcc
     7741 accaaaggca cacgggccct agaggcaacc acagagcaca tacgtcagga actggcggtc
     7801 ttctgttccc cagagccacc tgccaagacc tctacccctg aagacttcat ccgaatgacc
     7861 aagggtatta ctatggcaac agccaaagcc gttgctgctg gcaattcctg tcgacaggaa
     7921 gatgtcattg ccacagccaa tctgagccga cgggctattg cggacatgct tcgggcttgc
     7981 aaggaagcag ctttccaccc agaagtggcg cctgatgtac ggctccgagc cctgcactat
     8041 ggccgggagt gtgccaatgg ttacctggaa ctgctggacc acgtgctgct gacccttcag
     8101 aagccaaacc cagacctgaa gcagcagcta actggacact caaagcgggt tgctggctct
     8161 gtgactgagc tcatccaggc tgctgaagcc atgaaaggaa cagagtgggt ggacccagag
     8221 gaccctactg tcattgctga gaatgagctc ttgggagccg cagccgccat cgaggctgca
     8281 gccaagaagc tggagcagct gaagcccagg gccaaaccca aggaggcgga tgagtccttg
     8341 aactttgagg aacaaatcct agaagctgcc aagtccatcg ctgcagccac cagtgcactg
     8401 gtaaaggctg cgtcagcagc ccagagggag ctggtggctc aaggaaaggt gggcgccatt
     8461 ccagccaatg cactggatga tgggcagtgg tcgcagggcc tcatttccgc tgcccgtatg
     8521 gtggctgcgg ccaccaacaa tctgtgtgaa gcagctaacg cagctgtcca gggccatgct
     8581 agccaggaga aactcatttc ctcagccaag caagtagctg cctccacagc tcagctcctg
     8641 gtagcttgca aggtcaaagc agatcaggac tctgaggcaa tgaaacggct ccaggctgct
     8701 ggcaatgcag tgaagagggc ctcagataac ctggtaaagg cggcccagaa agctgcagcc
     8761 ttcgaagacc aggagaatga gacggtggtg gtgaaggaga aaatggttgg gggcattgcc
     8821 cagattatcg cagcacagga agagatgctt cggaaggaac gagagctgga agaggctcgg
     8881 aaaaagctcg cccagatccg gcagcagcag tacaagttct tgccttcaga gcttcgagac
     8941 gagcactaaa aaagccctgt gtatttaatg cagacccagc ccagagactg tgcctgccac
     9001 taccaaagcc ttctgggctg ctcaggaccc aagctgccca accccagcac tcccccaaag
     9061 tgcctgccaa accctgggcc tggccctgcc cagtcccact gcaagccgtg tcctctccct
     9121 gactcccaag tgcctttgca ccctagggcc cctaagtgcc tgcccccctc cagagtatta
     9181 acgctccaag agtattatta atgctgccgt acctcagttt gaacctgcca gggccccagc
     9241 tgctccagcc tgccagcagc ttccagccag tccccacggc cacgtcagct caactcatcc
     9301 ctttttgata ctatatcccc tacccagcta cctatggggc ttgagggttg taaacccaaa
     9361 caggtcagac tcccggaatt ctgcagtcga cggtaccgcg ggcccgggat ccaccggatc
     9421 tagataactg atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa
     9481 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt
     9541 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     9601 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttaacg
     9661 cgtaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct
     9721 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg
     9781 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact
     9841 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac
     9901 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga
     9961 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga
    10021 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca
    10081 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc aggtggcact tttcggggaa
    10141 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
    10201 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt cctgaggcgg
    10261 aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
    10321 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
    10381 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
    10441 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
    10501 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
    10561 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaag atcgatcaag
    10621 agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg
    10681 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg
    10741 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc
    10801 tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga
    10861 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc
    10921 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag
    10981 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat
    11041 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg
    11101 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca
    11161 ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct
    11221 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg
    11281 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg
    11341 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc
    11401 gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat
    11461 gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta
    11521 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
    11581 ggatctcatg ctggagttct tcgcccaccc tagggggagg ctaactgaaa cacggaagga
    11641 gacaataccg gaaggaaccc gcgctatgac ggcaataaaa agacagaata aaacgcacgg
    11701 tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact ctgtcgatac
    11761 cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc caccccaccc
    11821 cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag gccctgccat
    11881 agcctcaggt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
    11941 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
    12001 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
    12061 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
    12121 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
    12181 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
    12241 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
    12301 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
    12361 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
    12421 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
    12481 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
    12541 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
    12601 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
    12661 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
    12721 tgtggataac cgtattaccg ccatgcatta gttattaata gtaatcaatt acggggtcat
    12781 tagttcatag cccatatatg gagttccg
//
