LOCUS       sequence-19107-e         685 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid was designed for screening purposes and may contain 
            discrepancies relative to the current canonical GenBank version of 
            the gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, 
            Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW
  TITLE     Identification of poxvirus CD8+ T cell determinants to enable 
            rational design and characterization of smallpox vaccines.
  JOURNAL   J Exp Med. 2005 January 3; 201(1): 95–104.
  PUBMED    15623576
REFERENCE   2  (bases 1 to 685)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "2005-?-3"; volume: " 201(1)"; pages: " 95–104"
COMMENT     Sequence Label: DCT.VV.Orf23
FEATURES             Location/Qualifiers
     source          1..685
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             463..504
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             514..531
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(554..571)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    560..671
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ccgaattcca gctgaccacc atggtatctg ttaccataat tcctttgatg gctgatagtg
       61 tcagagcaca agatttccaa tctttgacaa tttttagcac cattatcttt gttttgatat
      121 ctatatcaga cagcatggtg cgtctgacaa cacaaggatt aagacggaaa gatgaaatga
      181 ttctctcaac atcttcaatg gataccttgc tattttttct ggcattatct atatgtgcga
      241 gaatatcctc tagagaatca gtatcctttt tgatgatagt ggatctcaat gacatgggac
      301 gtctaaacct tcttattcta tcaccagatt gcatggtgat ttgtcttctt tcttttatca
      361 taatgtaagc aattcccggg gatccgaata aagggtcaag acaattctgc agatatccag
      421 cacagtggcg gccgctcgag tctagagggc ccgcggttcg aaggtaagcc tatccctaac
      481 cctctcctcg gtctcgattc tacgcgtacc ggtcatcatc accatcacca ttgagtttaa
      541 acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
      601 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
      661 gaaattgcat cgcattgtct gagta
//
