LOCUS       sequence-191071-         752 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tet/Dox inducible (TetR) constitutively active mutant MKK6/MAP2K6 in
            lentiviral vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Frank SB, Berger PL, Ljungman M, Miranti CK
  TITLE     Human prostate luminal cell differentiation requires NOTCH3 
            induction by p38-MAPK and MYC.
  JOURNAL   J Cell Sci. 2017 Apr 26. pii: jcs.197152. doi: 10.1242/jcs.197152.
  PUBMED    28446540
REFERENCE   2  (bases 1 to 752)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell Sci.
            2017 Apr 26. pii: jcs.197152. doi: 10.1242/jcs.197152."
COMMENT     Sequence Label: pLenti-Tet-MKK6(DD)-Puro
FEATURES             Location/Qualifiers
     source          1..752
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    11..29
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    32..50
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    91..115
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 agcagagctc tccctatcag tgatagagat ctccctatca gtgatagaga tcgtcgacta
       61 gtccagtgtg gtggaattct gcagatatca acaagtttgt acaaaaaagc aggctcttta
      121 aaggaaccaa ttcagtcgac tggatccggt accgaattcg gccccccccc ccttgccgca
      181 tccacgaaac tttgcccata gcagcgggcg ggcactttgc actggaactt acaacacccg
      241 agcaaggacg cgactctccc gacgcgggga ggctattctg cccatttggg gacacttccc
      301 cgccgctgcc aggacccgct tctctgaaag gctctccttg cagctgctta gacgctggat
      361 ttttttcggg tagtggaaaa ccagcctccc gcgacgatgc ccctcaacgt tagcttcacc
      421 aacaggaact atgacctcga ctacgactcg gtgcagccgt atttctactg cgacgaggag
      481 gagaacttct accagcagca gcagcagagc gagctgcagc ccccggcgcc cagcgaggat
      541 atctggaaga aattcgagct gctgcccacc ccgcccctgt cccctagccg ccgctccggg
      601 ctctgctcgc cctcctacgt tgcggtcaca cccttctccc ttcggggaga caacgacggc
      661 ggtggcggga gcttctccac ggccgaccag ctggagatgg tgaccgagct gctgggagga
      721 gacatggtga accagagttt catctgcgac cc
//
