LOCUS       sequence-191082-        8242 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module A, Promoter: EC1.2, Gene: TREX2-P2A-AtCas9, Terminator: HSP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8242)
  AUTHORS   Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E, 
            Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF
  TITLE     A multi-purpose toolkit to enable advanced genome engineering in 
            plants.
  JOURNAL   Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 
            10.1105/tpc.16.00922.
  PUBMED    28522548
REFERENCE   2  (bases 1 to 8242)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922."
COMMENT     Sequence Label: pMOD_A0908
FEATURES             Location/Qualifiers
     source          1..8242
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      80..668
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     569..588
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     822..839
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             2619..2675
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2676..6779
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             2676..6779
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             6792..6812
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      6825..7073
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     promoter        7150..7254
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gccttgacgg caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
       61 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
      121 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ctaactcttt
      181 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
      241 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
      301 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
      361 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgctcacagc
      421 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
      481 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
      541 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
      601 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
      661 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
      721 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
      781 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
      841 aagcggaaga gcgcccacct gctcatgatc ggcgcgccac tttgataaat gttcctcgct
      901 gacgtaagaa gacattagta atggttataa tatatagctt tctatgaatg tatggtgaga
      961 aaatgtctgt tcactgattt tgagtttgga ataaaagcat ttgcgtttgg tttatcattg
     1021 cgtttataca aggacagaga tccactgagc tggaatagct taaaaccatt atcagaacaa
     1081 aataaaccat tttttgttaa gaatcagagc atagtaaaca acagaaacaa cctaagagag
     1141 gtaacttgtc caagaagata gctaattata tctattttat aaaagttatc atagtttgta
     1201 agtcacaaaa gatgcaaata acagagaaac taggagactt gagaatatac attcttgtat
     1261 atttgtattc gagattgtga aaatttgacc ataagtttaa attcttaaaa agatatatct
     1321 gatctagatg atggttatag actgtaattt taccacatgt ttaatgatgg atagtgacac
     1381 acatgacaca tcgacaacac tatagcatct tatttagatt acaacatgaa atttttctgt
     1441 aatacatgtc tttgtacata atttaaaagt aattcctaag aaatatattt atacaaggag
     1501 tttaaagaaa acatagcata aagttcaatg agtagtaaaa accatataca gtatatagca
     1561 taaagttcaa tgagtttatt acaaaagcat tggttcactt tctgtaacac gacgttaaac
     1621 cttcgtctcc aataggagcg ctactgattc aacatgccaa tatatactaa atacgtttct
     1681 acagtcaaat gctttaacgt ttcatgatta agtgactatt taccgtcaat cctttcccat
     1741 tcctcccact aatccaactt tttaattact cttaaatcac cactaagctt cgaatccatc
     1801 caaaaccaca atataaaaac agaactctcg taactcaatc atcgcaaaac aaaacaaaac
     1861 aaaacaaaaa ccccaaaaag aaagaataac ctgcaggaaa aatgtctgag ccacctcggg
     1921 ctgagacctt tgtattcctg gacctagaag ccactgggct cccaaacatg gaccctgaga
     1981 ttgcagagat atcccttttt gctgttcacc gctcttccct ggagaaccca gaacgggatg
     2041 attctggttc cttggtgctg ccccgtgttc tggacaagct cacactgtgc atgtgcccgg
     2101 agcgcccctt tactgccaag gccagtgaga ttactggttt gagcagcgaa agcctgatgc
     2161 actgcgggaa ggctggtttc aatggcgctg tggtaaggac actgcagggc ttcctaagcc
     2221 gccaggaggg ccccatctgc cttgtggccc acaatggctt cgattatgac ttcccactgc
     2281 tgtgcacgga gctacaacgt ctgggtgccc atctgcccca agacactgtc tgcctggaca
     2341 cactgcctgc attgcggggc ctggaccgtg ctcacagcca cggcaccagg gctcaaggcc
     2401 gcaaaagcta cagcctggcc agtctcttcc accgctactt ccaggctgaa cccagtgctg
     2461 cccattcagc agaaggtgat gtgcacaccc tgcttctgat cttcctgcat cgtgctcctg
     2521 agctgctcgc ctgggcagat gagcaggccc gcagctgggc tcatattgag cccatgtacg
     2581 tgccacctga tggtccaagc ctcgaagccg gatctggagc tactaatttt tctcttctta
     2641 agcaagctgg agatgttgaa gaaaatcctg gacccatgga taagaagtac tctatcggac
     2701 tcgatatcgg aactaactct gtgggatggg ctgtgatcac cgatgagtac aaggtgccat
     2761 ctaagaagtt caaggttctc ggaaacaccg ataggcactc tatcaagaaa aaccttatcg
     2821 gtgctctcct cttcgattct ggtgaaactg ctgaggctac cagactcaag agaaccgcta
     2881 gaagaaggta caccagaaga aagaacagga tctgctacct ccaagagatc ttctctaacg
     2941 agatggctaa agtggatgat tcattcttcc acaggctcga agagtcattc ctcgtggaag
     3001 aagataagaa gcacgagagg caccctatct tcggaaacat cgttgatgag gtggcatacc
     3061 acgagaagta ccctactatc taccacctca gaaagaagct cgttgattct actgataagg
     3121 ctgatctcag gctcatctac ctcgctctcg ctcacatgat caagttcaga ggacacttcc
     3181 tcatcgaggg tgatctcaac cctgataact ctgatgtgga taagttgttc atccagctcg
     3241 tgcagaccta caaccagctt ttcgaagaga accctatcaa cgcttcaggt gtggatgcta
     3301 aggctatcct ctctgctagg ctctctaagt caagaaggct tgagaacctc attgctcagc
     3361 tccctggtga gaagaagaac ggacttttcg gaaacttgat cgctctctct ctcggactca
     3421 cccctaactt caagtctaac ttcgatctcg ctgaggatgc aaagctccag ctctcaaagg
     3481 atacctacga tgatgatctc gataacctcc tcgctcagat cggagatcag tacgctgatt
     3541 tgttcctcgc tgctaagaac ctctctgatg ctatcctcct cagtgatatc ctcagagtga
     3601 acaccgagat caccaaggct ccactctcag cttctatgat caagagatac gatgagcacc
     3661 accaggatct cacacttctc aaggctcttg ttagacagca gctcccagag aagtacaaag
     3721 agattttctt cgatcagtct aagaacggat acgctggtta catcgatggt ggtgcatctc
     3781 aagaagagtt ctacaagttc atcaagccta tcctcgagaa gatggatgga accgaggaac
     3841 tcctcgtgaa gctcaataga gaggatcttc tcagaaagca gaggaccttc gataacggat
     3901 ctatccctca tcagatccac ctcggagagt tgcacgctat ccttagaagg caagaggatt
     3961 tctacccatt cctcaaggat aacagggaaa agattgagaa gattctcacc ttcagaatcc
     4021 cttactacgt gggacctctc gctagaggaa actcaagatt cgcttggatg accagaaagt
     4081 ctgaggaaac catcacccct tggaacttcg aagaggtggt ggataagggt gctagtgctc
     4141 agtctttcat cgagaggatg accaacttcg ataagaacct tccaaacgag aaggtgctcc
     4201 ctaagcactc tttgctctac gagtacttca ccgtgtacaa cgagttgacc aaggttaagt
     4261 acgtgaccga gggaatgagg aagcctgctt ttttgtcagg tgagcaaaag aaggctatcg
     4321 ttgatctctt gttcaagacc aacagaaagg tgaccgtgaa gcagctcaaa gaggattact
     4381 tcaagaaaat cgagtgcttc gattcagttg agatttctgg tgttgaggat aggttcaacg
     4441 catctctcgg aacctaccac gatctcctca agatcattaa ggataaggat ttcttggata
     4501 acgaggaaaa cgaggatatc ttggaggata tcgttcttac cctcaccctc tttgaagata
     4561 gagagatgat tgaagaaagg ctcaagacct acgctcatct cttcgatgat aaggtgatga
     4621 agcagttgaa gagaagaaga tacactggtt ggggaaggct ctcaagaaag ctcattaacg
     4681 gaatcaggga taagcagtct ggaaagacaa tccttgattt cctcaagtct gatggattcg
     4741 ctaacagaaa cttcatgcag ctcatccacg atgattctct cacctttaaa gaggatatcc
     4801 agaaggctca ggtttcagga cagggtgata gtctccatga gcatatcgct aacctcgctg
     4861 gatctcctgc aatcaagaag ggaatcctcc agactgtgaa ggttgtggat gagttggtga
     4921 aggtgatggg aaggcataag cctgagaaca tcgtgatcga aatggctaga gagaaccaga
     4981 ccactcagaa gggacagaag aactctaggg aaaggatgaa gaggatcgag gaaggtatca
     5041 aagagcttgg atctcagatc ctcaaagagc accctgttga gaacactcag ctccagaatg
     5101 agaagctcta cctctactac ctccagaacg gaagggatat gtatgtggat caagagttgg
     5161 atatcaacag gctctctgat tacgatgttg atcatatcgt gccacagtca ttcttgaagg
     5221 atgattctat cgataacaag gtgctcacca ggtctgataa gaacaggggt aagagtgata
     5281 acgtgccaag tgaagaggtt gtgaagaaaa tgaagaacta ttggaggcag ctcctcaacg
     5341 ctaagctcat cactcagaga aagttcgata acttgactaa ggctgagagg ggaggactct
     5401 ctgaattgga taaggcagga ttcatcaaga ggcagcttgt ggaaaccagg cagatcacta
     5461 agcacgttgc acagatcctc gattctagga tgaacaccaa gtacgatgag aacgataagt
     5521 tgatcaggga agtgaaggtt atcaccctca agtcaaagct cgtgtctgat ttcagaaagg
     5581 atttccaatt ctacaaggtg agggaaatca acaactacca ccacgctcac gatgcttacc
     5641 ttaacgctgt tgttggaacc gctctcatca agaagtatcc taagctcgag tcagagttcg
     5701 tgtacggtga ttacaaggtg tacgatgtga ggaagatgat cgctaagtct gagcaagaga
     5761 tcggaaaggc taccgctaag tatttcttct actctaacat catgaatttc ttcaagaccg
     5821 agattaccct cgctaacggt gagatcagaa agaggccact catcgagaca aacggtgaaa
     5881 caggtgagat cgtgtgggat aagggaaggg atttcgctac cgttagaaag gtgctctcta
     5941 tgccacaggt gaacatcgtt aagaaaaccg aggtgcagac cggtggattc tctaaagagt
     6001 ctatcctccc taagaggaac tctgataagc tcattgctag gaagaaggat tgggacccta
     6061 agaaatacgg tggtttcgat tctcctaccg tggcttactc tgttctcgtt gtggctaagg
     6121 ttgagaaggg aaagagtaag aagctcaagt ctgttaagga acttctcgga atcactatca
     6181 tggaaaggtc atctttcgag aagaacccaa tcgatttcct cgaggctaag ggatacaaag
     6241 aggttaagaa ggatctcatc atcaagctcc caaagtactc actcttcgaa ctcgagaacg
     6301 gtagaaagag gatgctcgct tctgctggtg agcttcaaaa gggaaacgag cttgctctcc
     6361 catctaagta cgttaacttt ctttacctcg cttctcacta cgagaagttg aagggatctc
     6421 cagaagataa cgagcagaag caacttttcg ttgagcagca caagcactac ttggatgaga
     6481 tcatcgagca gatctctgag ttctctaaaa gggtgatcct cgctgatgca aacctcgata
     6541 aggtgttgtc tgcttacaac aagcacagag ataagcctat cagggaacag gcagagaaca
     6601 tcatccatct cttcaccctt accaacctcg gtgctcctgc tgctttcaag tacttcgata
     6661 caaccatcga taggaagaga tacacctcta ccaaagaagt gctcgatgct accctcatcc
     6721 atcagtctat cactggactc tacgagacta ggatcgatct ctcacagctc ggtggtgatt
     6781 caagggctga tcctaagaag aagaggaagg tttgacgtcg acgatatgaa gatgaagatg
     6841 aaatatttgg tgtgtcaaat aaaaagcttg tgtgcttaag tttgtgtttt tttcttggct
     6901 tgttgtgtta tgaatttgtg gctttttcta atattaaatg aatgtaagat cacattataa
     6961 tgaataaaca aatgtttcta taatccattg tgaatgtttt gttggatctc ttctgcagca
     7021 tataactact gtatgtgcta tggtatggac tatggaatat gattaaagat aagccagagc
     7081 tctggtgacg gacgtgggca ggtgtgatga aaagccttag ggtcaggtgg cacttttcgg
     7141 ggaaatgtgc gatgaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     7201 ctaatgagac aataaccctg ataaatgctt caattatatt gaaaaaggaa gagtatgagt
     7261 atccaacatt tccgtgtcgc ccttattccc ttttttgcgg ccttctgcct tcctgttttt
     7321 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcccgagtg
     7381 ggttacattg aactggatct caacagcggc aaaatcttag agagttttcg ccccgaagaa
     7441 cgcttcccaa tgatgagcac tttcaaagtt ctgctatgtg gcgcggtgtt atcccgtatt
     7501 gatgctgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     7561 tattcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgtagt
     7621 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gattggagga
     7681 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     7741 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     7801 gcaatggcaa caactctccg caaactatta actggcgaac tacttactct tgcttcacgc
     7861 cagcaactca ttgactggat ggaggcggac aaagttgcag gaccacttct gcgctcggca
     7921 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     7981 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     8041 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     8101 attaagcatt ggtaactgtc agacgaaggg aaataaatag tagcccgcct gatgtgcggg
     8161 cttttttttg gtggtggtta ccagtgtcgc ttagctaccg taggtcctct taggcggtcc
     8221 gattagcgac atagtcatca at
//
