LOCUS       sequence-19110-e         698 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid was designed for screening purposes and may contain 
            discrepancies relative to the current canonical GenBank version of 
            the gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, 
            Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW
  TITLE     Identification of poxvirus CD8+ T cell determinants to enable 
            rational design and characterization of smallpox vaccines.
  JOURNAL   J Exp Med. 2005 January 3; 201(1): 95–104.
  PUBMED    15623576
REFERENCE   2  (bases 1 to 698)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "2005-?-3"; volume: " 201(1)"; pages: " 95–104"
COMMENT     Sequence Label: DCT.VV.Orf27
FEATURES             Location/Qualifiers
     source          1..698
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             563..604
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             614..631
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(654..671)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 ggttaatttt atcaagacat atgcgcctag aggttctatt atttttatta attataccat
       61 gtcattaaca agtcatttga atccatcgat agaaaaacat gtgggtattt attatggtac
      121 gttattatcg gaacacttgg tagttgaatc aacatataga aaaggagttc gaatagtccc
      181 attggatagt ttttttgaag gatatcttag tgcaaaagta tacatgttag agaatattca
      241 agttatgaaa atagcagctg atacgtcatt aactttattg ggtattccgt atggatttgg
      301 tcatgataga atgtattgtt ttaaattggt agctgactgt tataaaaatg ccggtattga
      361 tacatcgtct aaacgaatat taggtaaaga tatttttctg agccaaaact tcacagacga
      421 taatagatgg ataaagatat atgattctaa taatttaaca ttttggcaaa ttgattacct
      481 taaagggtga aagggtcaag acaattctgc agatatccag cacagtggcg gccgctcgag
      541 tctagagggc ccgcggttcg aaggtaagcc tatccctaac cctctcctcg gtctcgattc
      601 tacgcgtacc ggtcatcatc accatcacca ttgagtttaa acccgctgat cagcctcgac
      661 tgtgccttct agttgccagc catctgttgt ttgcccct
//
