LOCUS       sequence-191112-        4050 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CpG free plasmid for testing effects of methylation on regulatory 
            activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4050)
  AUTHORS   Tung
  TITLE     Tung lab mSTARR-seq plasmud
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4050)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pmSTARRseq1
FEATURES             Location/Qualifiers
     source          1..4050
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          1005..1234
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     1217..1236
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             1282..1998
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQRNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     polyA_signal    2601..2722
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2612..2631)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2666..2685
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 agctcatcta ggcctctgac ccagacccaa gcaagggtgt tgtcagggac aacttggtcc
       61 tgaactgctg agatgaagag ggtgacatca tctctgacaa caccagcaaa atcatcttca
      121 acaaagtctc tggagaatcc taatctgtca gtccagaact ctacagcccc tgcaacatcc
      181 cttgctgtga ggactgggac tgcagaagtg agtttggcca tgatggccct cctatagtga
      241 gttgtattat actatgcaga tatactatgc caatgtttaa ttgtcaacta cctgttttaa
      301 ttaaaattat ctctaaggca tgtgaactgg ctgtcttggt tttcatctgt acttcatctg
      361 ctacctctgt gacctgaaac atatttataa ttccattaag ctgtgcatat gatagattta
      421 tcatatgtat tttccttaaa ggatttttgt aagaactaat tgaattgata cctgtaaagt
      481 ctttatcaca ctacccaata aataataaat ctctttgttc agctctctgt ttctataaat
      541 atgtaccagt tttattgttt ttagtggtag tgattttatt ctctttctat atatatacac
      601 acacatgtgt gcattcataa atatatacaa tttttatgaa taaaaaatta ttagcaatca
      661 atattgaaaa ccactgattt ttgtttatgt gagcaaacag cagattaaaa ggaattcctg
      721 cagggcccac ctaggatgca tagggagaag agcatgcttg agggctgagt gcccctcagt
      781 gggcagagag cacatggccc acagtccctg agaagttggg gggaggggtg ggcaattgaa
      841 ctggtgccta gagaaggtgg ggcttgggta aactgggaaa gtgatgtggt gtactggctc
      901 cacctttttc cccagggtgg gggagaacca tatataagtg cagtagtctc tgtgaacatt
      961 cagggcctga attaattcac tgtctgccag ggccagctgt tggggtgagt actccctctc
     1021 aaaagctggc atgacttctg cactaagatt gtcagtttcc aaaaatgagg aggatttgat
     1081 attcaactgg ccctcagtga tgcctttgag ggtggctggg tccatctggt cagaaaagac
     1141 aatctttttg ttgtcaagct tgaggtgtgg caggcttgag gtctggccat acacttgagt
     1201 gacaatgaca tccactttgc ctttctctcc acaggtgtcc actcccaggt ccaactgcag
     1261 gtcacctgca ggcttaagct catggtttct aagggagaag aactctttac tggtgttgtc
     1321 ccaattctgg ttgagctgga tggtgatgtg aatggccaca aattctctgt gtctggtgaa
     1381 ggtgaaggag atgcaactta tggaaagctg actctgaagt tcatttgtac aacaggaaag
     1441 ctgccagtgc cttggccaac tctggtgacc accctgactt atggtgttca atgtttcagc
     1501 aggtaccctg accacatgaa gcagcatgac ttctttaaat ctgcaatgcc agaaggttat
     1561 gttcaggaga ggacaatctt ctttaaggat gatggaaatt ataagacaag ggcagaagtg
     1621 aagtttgaag gtgatacact ggttaacaga attgagctga aaggcattga ttttaaggaa
     1681 gatggaaaca ttctgggtca caagctggag tacaactata attctcacaa tgtttacatt
     1741 atggcagata agcagaggaa tggaattaag gctaatttca agattagaca caacattgag
     1801 gatgggtctg tccaactggc agaccattac cagcagaaca cccctattgg tgatggccca
     1861 gttctcctcc cagataatca ctatctcagc actcaatctg ctctgtccaa agaccctaat
     1921 gagaaaagag accacatggt cctcctggag tttgtgacag cagcaggaat tactctggga
     1981 atggatgagc tgtacaaggc caagttgacc agtgctgtcc cagtgctcac agccagggat
     2041 gtggctggag ctgttgagtt ctggactgac aggttggggt tctccagaga ttttgtggag
     2101 gatgactttg caggtgtggt cagagatgat gtcaccctgt tcatctcagc agtccaggac
     2161 caggtggtgc ctgacaacac cctggcttgg gtgtgggtga gaggactgga tgagctgtat
     2221 gctgagtgga gtgaggtggt ctccaccaac ttcagggatg ccagtggccc tgccatgaca
     2281 gagattggag agcagccctg ggggagagag tttgccctga gagacccagc aggcaactgt
     2341 gtgcactttg tggcagagga gcaggactaa agtctagagc ttgtactagt ggtgattccc
     2401 ctgacgcgtg cacgtctgct gacgcgtaaa gtctcccgtg aactttaccc acgcgtgcat
     2461 atcggggatg aaagacgcgt catgatgacc accgatatgg ccagtgtacg cgtctcgggg
     2521 aatataaata cacagccagt ctgcaggaca tcccatggga attcagccag ccacttcaac
     2581 tgctagctgg ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat
     2641 gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat
     2701 tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca
     2761 gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtatggaatt
     2821 cagtcaatat gttcacccca aaaaagctgt ttgttaactt gccaacctca ttctaaaatg
     2881 tatatagaag cccaaaagac aataacaaaa atattcttgt agaacaaaat gggaaagaat
     2941 gttccactaa atatcaagat ttagagcaaa gcatgagatg tgtggggata gacagtgagg
     3001 ctgataaaat agagtagagc tcagaaacag acccattgat atatgtaagt gacctatgaa
     3061 aaaaatatgg cattttacaa tgggaaaatg atggtctttt tcttttttag aaaaacaggg
     3121 aaatatattt atatgtaaaa aataaaaggg aacccatatg tcataccata cacacaaaaa
     3181 aattccagtg aattataagt ctaaatggag aaggcaaaac tttaaatctt ttagaaaata
     3241 atatagaagc atgccatcaa gacttcagtg tagagaaaaa tttcttatga ctcaaagtcc
     3301 taaccacaaa gaaaagattg ttaattagat tgcatgaata ttaagactta tttttaaaat
     3361 taaaaaacca ttaagaaaag tcaggccata gaatgacaga aaatatttgc aacaccccag
     3421 taaagagaat tgtaatatgc agattataaa aagaagtctt acaaatcagt aaaaaataaa
     3481 actagacaaa aatttgaaca gatgaaagag aaactctaaa taatcattac acatgagaaa
     3541 ctcaatctca gaaatcagag aactatcatt gcatatacac taaattagag aaatattaaa
     3601 aggctaagta acatctgtgg cttaattaaa atcagcagtt caacctgttg atagtatgta
     3661 ctaagctctc atgtttaatg tactaagctc tcatgtttaa tgaactaaac cctcatggct
     3721 aatgtactaa gctctcatgg ctaatgtact aagctctcat gtttcatgta ctaagctctc
     3781 atgtttgaac aataaaatta atataaatca gcaacttaaa tagcctctaa ggttttaagt
     3841 tttataagaa aaaaaagaat atataaggct tttaaaggtt ttaaggtttc ctagctttag
     3901 tcctgttcct cagctacaaa atggacacaa tttccagcag ggtctctgag ggcaaattcc
     3961 cttccccaag gttgttcacc aatttctgtc atggctgggc cagaggcatc cctgaaattt
     4021 gtgctgacta cttctgacca ttctgcataa
//
