LOCUS       sequence-191119-        2432 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CMYA5 (NM_153610, nt 12205 to 12279) T allele (rs10043986) in 
            pENTR3C, Gateway Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Hsiung A, Naya FJ, Chen X, Shiang R
  TITLE     A schizophrenia associated CMYA5 allele displays differential 
            binding with desmin.
  JOURNAL   J Psychiatr Res. 2019 Apr;111:8-15. doi: 
            10.1016/j.jpsychires.2019.01.007. Epub 2019 Jan 10.
  PUBMED    30658136
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Psychiatr
            Res."; date: "2019-04"; volume: "111:8-15. doi"; pages: " 
            10.1016/j.jpsychires.2019.01.007. Epub 2019 Jan 10"
COMMENT     Sequence Label: CMYA5T2/pENTR3C
FEATURES             Location/Qualifiers
     source          1..2432
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(62..148)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      complement(313..901)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(393..412)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(991..1800)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1714..1733
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1792..1811
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     protein_bind    1923..2022
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     2032..2048
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     2033..2049
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2226..2325)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2344..2367)
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     terminator      complement(2375..2402)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc gctcccggcg
       61 gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt
      121 cttccgactg agcctttcgt tttatttgat gcctggcagt tccctactct cgcttagtag
      181 ttagacgtcc ccgagatcca tgctagcggt aatacggtta tccacagaat caggggataa
      241 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
      301 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
      361 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
      421 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
      481 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
      541 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
      601 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
      661 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
      721 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
      781 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
      841 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
      901 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgggg cccaatctga
      961 ataatgttac aaccaattaa ccaattctga ttagaaaaac tcatcgagca tcaaatgaaa
     1021 ctgcaattta ttcatatcag gattatcaat accatatttt tgaaaaagcc gtttctgtaa
     1081 tgaaggagaa aactcaccga ggcagttcca taggatggca agatcctggt atcggtctgc
     1141 gattccgact cgtccaacat caatacaacc tattaatttc ccctcgtcaa aaataaggtt
     1201 atcaagtgag aaatcaccat gagtgacgac tgaatccggt gagaatggca aaagtttatg
     1261 catttctttc cagacttgtt caacaggcca gccattacgc tcgtcatcaa aatcactcgc
     1321 atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg agacgaaata cgcgatcgct
     1381 gttaaaagga caattacaaa caggaatcga atgcaaccgg cgcaggaaca ctgccagcgc
     1441 atcaacaata ttttcacctg aatcaggata ttcttctaat acctggaatg ctgtttttcc
     1501 ggggatcgca gtggtgagta accatgcatc atcaggagta cggataaaat gcttgatggt
     1561 cggaagaggc ataaattccg tcagccagtt tagtctgacc atctcatctg taacatcatt
     1621 ggcaacgcta cctttgccat gtttcagaaa caactctggc gcatcgggct tcccatacaa
     1681 gcgatagatt gtcgcacctg attgcccgac attatcgcga gcccatttat acccatataa
     1741 atcagcatcc atgttggaat ttaatcgcgg cctcgacgtt tcccgttgaa tatggctcat
     1801 aacacccctt gtattactgt ttatgtaagc agacagtttt attgttcatg atgatatatt
     1861 tttatcttgt gcaatgtaac atcagagatt ttgagacacg ggccagagct gcagctggat
     1921 ggcaaataat gattttattt tgactgatag tgacctgttc gttgcaacaa attgataagc
     1981 aatgctttct tataatgcca actttgtaca agaaagctgg gtctagatat ctcgaggtcg
     2041 acggtatcga taagcttgat tgaattcctt gtgccttaca gaatccaggg gctctatccc
     2101 caggtgcaaa gtacattttc caggtttctc cagggcaaag gcagggtgga caccctcatt
     2161 aaccatgggg attctgtaag gcacaaggaa ttcaatcgaa ttcctgcagc ccggttcctt
     2221 taaagagcct gcttttttgt acaaagttgg cattataaaa aagcattgct tatcaatttg
     2281 ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt atttggggcc cgagcttaag
     2341 taactaacta acaggaagag tttgtagaaa cgcaaaaagg ccatccgtca ggatggcctt
     2401 ctgcttagtt tgatgcctgg cagtttatgg cg
//
