LOCUS       sequence-191121-        4785 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For fusion gene cloning. Recombine Gene B into this Gateway Entry 
            Vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4785)
  AUTHORS   Lu H, Villafane N, Dogruluk T, Grzeskowiak CL, Kong K, Tsang YH, 
            Zagorodna O, Pantazi A, Yang L, Neill NJ, Kim YW, Creighton CJ, 
            Verhaak RG, Mills GB, Park P, Kucherlapati RS, Scott KL
  TITLE     Engineering and Functional Characterization of Fusion Genes 
            Identifies Novel Oncogenic Drivers of Cancer.
  JOURNAL   Cancer Res. 2017 May 16. pii: canres.2745.2016. doi: 
            10.1158/0008-5472.CAN-16-2745.
  PUBMED    28512244
REFERENCE   2  (bases 1 to 4785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer Res.
            2017 May 16. pii: canres.2745.2016. doi: 
            10.1158/0008-5472.CAN-16-2745."
COMMENT     Sequence Label: pFUSE-B_P2rP4
FEATURES             Location/Qualifiers
     source          1..4785
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     89..106
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(265..292)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(384..470)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     519..541
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     533..550
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     534..550
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(570..801)
                     /gene="mutant version of attP"
                     /label=attP2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(972..1277)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(1074..1093)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(1597..2280)
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGRKRR
                     VDPAY"
     primer_bind     2195..2214
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(2281..2383)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     protein_bind    complement(2528..2759)
                     /gene="mutant version of attP"
                     /label=attP4
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(2777..2796)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2778..2796)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2801..2817)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2801..2817)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3248..4039
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4132..4720
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4621..4640
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
       61 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
      121 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
      181 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatacgc gtaccgctag
      241 ccaggaagag tttgtagaaa cgcaaaaagg ccatccgtca ggatggcctt ctgcttagtt
      301 tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc ctccgggccg ttgcttcaca
      361 acgttcaaat ccgctcccgg cggatttgtc ctactcagga gagcgttcac cgacaaacaa
      421 cagataaaac gaaaggccca gtcttccgac tgagcctttc gttttatttg atgcctggca
      481 gttccctact ctcgcgttaa cgctagcatg gatgttttcc cagtcacgac gttgtaaaac
      541 gacggccagt cttaaggcgc ttagtcgact acaggtcact aataccatct aagtagttga
      601 ttcatagtga ctggatatgt tgtgttttac agtattatgt agtctgtttt ttatgcaaaa
      661 tctaatttaa tatattgata tttatatcat tttacgtttc tcgttcagct ttcttgtaca
      721 aagttggcat tataagaaag cattgcttat caatttgttg caacgaacag gtcactatca
      781 gtcaaaataa aatcattatt tgccatccag ctgatatccg aagaaatatt tcacaactct
      841 tatacttttc tcttacaagt cgttcggctt catctggatt ttcagcctct atacttacta
      901 aacgtgataa agtttctgta atttctactg tatcgacctg cagactggct gtgtataagg
      961 gagcctgaca tttatattcc ccagaacatc aggttaatgg cgtttttgat gtcattttcg
     1021 cggtggctga gatcagccac ttcttccccg ataacggaga ccggcacact ggccatatcg
     1081 gtggtcatca tgcgccagct ttcatccccg atatgcacca ccgggtaaag ttcacgggag
     1141 actttatctg acagcagacg tgcactggcc agggggatca ccatccgtcg cccgggcgtg
     1201 tcaataatat cactctgtac atccacaaac agacgataac ggctctctct tttataggtg
     1261 taaaccttaa actgcatttc accagcccct gttctcgtca gcaaaagagc cgttcatttc
     1321 aataaaccgg gcgacctcag ccatcccttc ctgattttcc gctttccagc gttcggcacg
     1381 cagacgacgg gcttcattct gcatggttgt gcttaccaga ccggagatat tgacatcata
     1441 tatgccttga gcaactgata gctgtcgctg tcaactgtca ctgtaatacg ctgcttcata
     1501 gcatacctct ttttgacata cttcgggtat acatatcagt atatattctt ataccgcaaa
     1561 aatcagcgcg caaatacgca tactgttatc tggcttttag taagccggat ccacgcggcg
     1621 tttacgcccc ccctgccact catcgcagta ctgttgtaat tcattaagca ttctgccgac
     1681 atggaagcca tcacaaacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc
     1741 gccttgcgta taatatttgc ccatggtgaa aacgggggcg aagaagttgt ccatattggc
     1801 cacgtttaaa tcaaaactgg tgaaactcac ccagggattg gctgagacga aaaacatatt
     1861 ctcaataaac cctttaggga aataggccag gttttcaccg taacacgcca catcttgcga
     1921 atatatgtgt agaaactgcc ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt
     1981 ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca ctatcccata tcaccagctc
     2041 accgtctttc attgccatac ggaattccgg atgagcattc atcaggcggg caagaatgtg
     2101 aataaaggcc ggataaaact tgtgcttatt tttctttacg gtctttaaaa aggccgtaat
     2161 atccagctga acggtctggt tataggtaca ttgagcaact gactgaaatg cctcaaaatg
     2221 ttctttacga tgccattggg atatatcaac ggtggtatat ccagtgattt ttttctccat
     2281 tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg gtagtgatct
     2341 tatttcatta tggtgaaagt tggaacctct tacgtgccga tcaacgtctc attttcgcca
     2401 aaagttggcc cagggcttcc cggtatcaac agggacacca ggatttattt attctgcgaa
     2461 gtgatcttcc gtcacaggta tttattcggc gcaaagtgcg tcgggtgatg ctgccaactt
     2521 agtcgactac aggtcactaa taccatctaa gtagttgatt catagtgact ggatatgttg
     2581 tgttttacag tattatgtag tctgtttttt atgcaaaatc taatttaata tattgatatt
     2641 tatatcattt tacgtttctc gttcaacttt tctatacaaa gttggcatta taagaaagca
     2701 ttgcttatca atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg
     2761 ccatccagct gatatcccct atagtgagtc gtattacatg gtcatagctg tttcctggca
     2821 gctctggccc gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc
     2881 atgcctcctc tagaccagcc aggacagaaa tgcctcgact tcgctgctac ccaaggttgc
     2941 cgggtgacgc acaccgtgga aacggatgaa ggcacgaacc cagtggacat aagcctgttc
     3001 ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc aactggtcca gaaccttgac
     3061 cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt
     3121 tggggtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg
     3181 tttgatgtta tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt
     3241 aaacattatg agggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg
     3301 cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt
     3361 ggatggcggc ctgaagccac acagtgatat tgatttgctg gttacggtga ccgtaaggct
     3421 tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg
     3481 agagagcgag attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc
     3541 gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct
     3601 tgcaggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc
     3661 aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc
     3721 tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga
     3781 ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt
     3841 aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc
     3901 ccagtatcag cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg
     3961 cttggcctcg cgcgcagatc agttggaaga atttgtccac tacgtgaaag gcgagatcac
     4021 caaggtagtc ggcaaataac cctcgagcca cccatgacca aaatccctta acgtgagtta
     4081 cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     4141 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     4201 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     4261 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     4321 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     4381 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     4441 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     4501 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     4561 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     4621 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     4681 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     4741 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctt
//
