LOCUS       sequence-191143-        8245 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CMYA5 (aa 4039 – 4069) in a GAL4 AD vector, used for yeast-two 
            hybrid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8245)
  AUTHORS   Hsiung A, Naya FJ, Chen X, Shiang R
  TITLE     A schizophrenia associated CMYA5 allele displays differential 
            binding with desmin.
  JOURNAL   J Psychiatr Res. 2019 Apr;111:8-15. doi: 
            10.1016/j.jpsychires.2019.01.007. Epub 2019 Jan 10.
  PUBMED    30658136
REFERENCE   2  (bases 1 to 8245)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Psychiatr
            Res."; date: "2019-04"; volume: "111:8-15. doi"; pages: " 
            10.1016/j.jpsychires.2019.01.007. Epub 2019 Jan 10"
COMMENT     Sequence Label: CMYA5/pACT2
FEATURES             Location/Qualifiers
     source          1..8245
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    112..145
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    223..256
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    300..333
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    411..444
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     terminator      complement(465..652)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     573..597
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(1208..1234)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             complement(1214..1240)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(1253..1594)
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="activation domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 activation domain
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     CDS             complement(1610..1630)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        complement(1676..2077)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     primer_bind     complement(2106..2128)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    2214..2354
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     2269..2288
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(2369..2386)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2540..3128)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2620..2639)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3299..4090,4091..4159))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3922..3941
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4160..4264)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4529..5871
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    4861..4908
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        6348..6753
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             6754..7848
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
ORIGIN
        1 ttcttgaacc atttcccata atggtgaaag ttccctcaag aattttactc tgtcagaaac
       61 ggccttaacg acgtagtcga ccaattccga tcatattcaa taacccttaa tataacttcg
      121 tataatgtat gctatacgaa gttattaggt ctgaagagga gtttacgtcc agccaagcta
      181 gcttggctgc aggtcgagcg gccgcgatcc ggaaccctta atataacttc gtataatgta
      241 tgctatacga agttattagg tccctcgacc aattccgatc atattcaata acccttaata
      301 taacttcgta taatgtatgc tatacgaagt tattaggtct gaagaggagt ttacgtccag
      361 ccaagctagc ttggctgcag gtcgagcggc cgcgatccgg aacccttaat ataacttcgt
      421 ataatgtatg ctatacgaag ttattaggtc cctcgaccgg catgccggta gaggtgtggt
      481 caataagagc gacctcatgc tatacctgag aaagcaacct gacctacagg aaagagttac
      541 tcaagaataa gaattttcgt tttaaaacct aagagtcact ttaaaatttg tatacactta
      601 ttttttttat aacttattta ataataaaaa tcataaatca taagaaattc gcttatttag
      661 aagtgtcaac aacgtatcta ccaacgattt gacccttttc catcttttcg taaatttctg
      721 gcaaggtaga caagccgaca accttgattg gagacttgac caaacctctg gcgaagaagt
      781 ccaaagcttc tgaataagcc ctcgtaatat attttcatga agaatttagg tccaaaaaaa
      841 agatgggcat taattctagt catttaaaaa attctataga tcagaggtta catggccaag
      901 attgaaactt agaggagtat agttacataa aagaaggcaa aacgatgtat aaatgaaaga
      961 aattgagatg gtgcacgatg cacagttgaa gtgaacttgc ggggtttttc agtatctacg
     1021 attcatagat ctctcgagct cgaattcgga tccccggggc ctccatgggg attctgtaag
     1081 gcacaaggaa ttccttgtgc cttacagaat ccgggggctc tatccccagg tgcaaagtac
     1141 attttccagg tttctccagg gcaaaggcag ggtggacacc ctcattaacc atggccatat
     1201 gaccacccaa gctagcgtaa tctggaacat cgtatgggta agccatagag atctcttttt
     1261 ttgggtttgg tggggtatct tcatcatcga atagatagtt atatacatca tccattgtag
     1321 tggtattaaa catccctgta gtgattccaa acgcgttata cgcagtttgg tccgtccaac
     1381 caggtgacag tggttttgaa ttattaccat catcaatttt actagccgtg atttcattat
     1441 tcatgaagtt atcatgaacg ttagaggagg caattggttg tgaaagcgct tgagaatttg
     1501 tttgagttgt tatgaggttc ggaccgttgc tactgttagt gaaagtgaag gacaatgagc
     1561 tatcagcaat attcccactt tgattaaaat tggcggcggt acccaattcg acctttctct
     1621 tcttttttgg aggctcggga attaattccg ctttatccat ctttgcaaag cttggagttg
     1681 attgtatgct tggtatagct tgaaatattg tgcagaaaaa gaaacaagga agaaagggaa
     1741 cgagaacaat gacgaggaaa caaaagatta ataattgcag gtctatttat acttgatagc
     1801 aagacagcaa actttttttt atttcaaatt caagtaactg gaaggaaggc cgtataccgt
     1861 tgctcattag agagtagtgt gcgtgaatga aggaaggaaa aagtttcgtg tgcttcgaga
     1921 tacccctcat cagctctgga acaacgacat ctgttggtgc tgtctttgtc gttaattttt
     1981 tcctttagtg tcttccatca tttttttgtc attgcggata tggtgagaca acaacggggg
     2041 agagagaaaa gaaaaaaaaa gaaaagaagt tgctgcctcg cgcgtttcgg tgatgacggt
     2101 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc
     2161 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc
     2221 atgacccagt cacgtagcga tagcggagtg tatactggct taactatgcg gcatcagagc
     2281 agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa
     2341 aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     2401 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     2461 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     2521 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     2581 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     2641 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     2701 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     2761 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     2821 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     2881 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     2941 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
     3001 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     3061 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     3121 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     3181 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     3241 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     3301 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     3361 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     3421 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     3481 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     3541 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     3601 ttgttgccat tgctgcaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     3661 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     3721 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     3781 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     3841 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     3901 cttgcccggc gtcaacacgg gataataccg cgccacatag cagaacttta aaagtgctca
     3961 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     4021 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     4081 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     4141 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     4201 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     4261 cgcgcacatt tccccgaaaa gtgccacctg agctttgaag aaaaatgcgc cttattcaat
     4321 ctttgctata aaaaatggcc caaaatctca cattggaaga catttgatga cctcatttct
     4381 ttcaatgaag ggcctaacgg agttgactaa tgttgtggga aattggagcg ataagcgtgc
     4441 ttctgccgtg gccaggacaa cgtatactca tcagataaca gcaatacctg atcactactt
     4501 cgcactagtt tctcggtact atgcatatga tccaatatca aaggaaatga tagcattgaa
     4561 ggatgagact aatccaattg aggagtggca gcatatagaa cagctaaagg gtagtgctga
     4621 aggaagcata cgataccccg catggaatgg gataatatca caggaggtac tagactacct
     4681 ttcatcctac ataaatagac gcatataagt acgcatttaa gcataaacac gcactatgcc
     4741 gttcttctca tgtatatata tatacaggca acacgcagat ataggtgcga cgtgaacagt
     4801 gagctgtatg tgcgcagctc gcgttgcatt ttcggaagcg ctcgttttcg gaaacgcttt
     4861 gaagttccta ttccgaagtt cctattctct agaaagtata ggaacttcag agcgcttttg
     4921 aaaaccaaaa gcgctctgaa gacgcacttt caaaaaacca aaaacgcacc ggactgtaac
     4981 gagctactaa aatattgcga ataccgcttc cacaaacatt gctcaaaagt atctctttgc
     5041 tatatatctc tgtgctatat ccctatataa cctacccatc cacctttcgc tccttgaact
     5101 tgcatctaaa ctcgacctct acatttttta tgtttatctc tagtattact ctttagacaa
     5161 aaaaattgta gtaagaacta ttcatagagt gaatcgaaaa caatacgaaa atgtaaacat
     5221 ttcctatacg tagtatatag agacaaaata gaagaaaccg ttcataattt tctgaccaat
     5281 gaagaatcat caacgctatc actttctgtt cacaaagtat gcgcaatcca catcggtata
     5341 gaatataatc ggggatgcct ttatcttgaa aaaatgcacc cgcagcttcg ctagtaatca
     5401 gtaaacgcgg gaagtggagt caggcttttt ttatggaaga gaaaatagac accaaagtag
     5461 ccttcttcta accttaacgg acctacagtg caaaaagtta tcaagagact gcattataga
     5521 gcgcacaaag gagaaaaaaa gtaatctaag atgctttgtt agaaaaatag cgctctcggg
     5581 atgcattttt gtagaacaaa aaagaagtat agattctttg ttggtaaaat agcgctctcg
     5641 cgttgcattt ctgttctgta aaaatgcagc tcagattctt tgtttgaaaa attagcgctc
     5701 tcgcgttgca tttttgtttt acaaaaatga agcacagatt cttcgttggt aaaatagcgc
     5761 tttcgcgttg catttctgtt ctgtaaaaat gcagctcaga ttctttgttt gaaaaattag
     5821 cgctctcgcg ttgcattttt gttctacaaa atgaagcaca gatgcttcgt taacaaagat
     5881 atgctattga agtgcaagat ggaaacgcag aaaatgaacc ggggatgcga cgtgcaagat
     5941 tacctatgca atagatgcaa tagtttctcc aggaaccgaa atacatacat tgtcttccgt
     6001 aaagcgctag actatatatt attatacagg ttcaaatata ctatctgttt cagggaaaac
     6061 tcccaggttc ggatgttcaa aattcaatga tgggtaacaa gtacgatcgt aaatctgtaa
     6121 aacagtttgt cggatattag gctgtatctc ctcaaagcgt attcgaatat cattgagaag
     6181 ctgcagcgtc acatcggata ataatgatgg cagccattgt agaagtgcct tttgcatttc
     6241 tagtctcttt ctcggtctag ctagttttac tacatcgcga agatagaatc ttagatcaca
     6301 ctgcctttgc tgagctggat caatagagta acaaaagagt ggtaaggaac tgtgggaata
     6361 ctcaggtatc gtaagatgca agagttcgaa tctcttagca accattattt ttttcctcaa
     6421 cataacgaga acacacaggg gcgctatcgc acagaatcaa attcgatgac tggaaatttt
     6481 ttgttaattt cagaggtcgc ctgacgcata tacctttttc aactgaaaaa ttgggagaaa
     6541 aaggaaaggt gagagcgccg gaaccggctt ttcatataga atagagaagc gttcatgact
     6601 aaatgcttgc atcacaatac ttgaagttga caatattatt taaggaccta ttgttttttc
     6661 caataggtgg ttagcaatcg tcttactttc taacttttct taccttttac atttcagcaa
     6721 tatatatata tatttcaagg atataccatt ctaatgtctg cccctaagaa gatcgtcgtt
     6781 ttgccaggtg accacgttgg tcaagaaatc acagccgaag ccattaaggt tcttaaagct
     6841 atttctgatg ttcgttccaa tgtcaagttc gatttcgaaa atcatttaat tggtggtgct
     6901 gctatcgatg ctacaggtgt tccacttcca gatgaggcgc tggaagcctc caagaaggct
     6961 gatgccgttt tgttaggtgc tgtgggtggt cctaaatggg gtacaggtag tgttagacct
     7021 gaacaaggtt tactaaaaat ccgtaaagaa cttcaattgt acgccaactt aagaccatgt
     7081 aactttgcat ccgactctct tttagactta tctccaatca agccacaatt tgctaaaggt
     7141 actgacttcg ttgttgtcag agaattagtg ggaggtattt actttggtaa gagaaaggaa
     7201 gacgatggtg atggtgtcgc ttgggatagt gaacaataca ccgttccaga agtgcaaaga
     7261 atcacaagaa tggccgcttt catggcccta caacatgagc caccattgcc tatttggtcc
     7321 ttggataaag ctaatgtttt ggcctcttca agattatgga gaaaaactgt ggaggaaacc
     7381 atcaagaacg aatttcctac attgaaggtt caacatcaat tgattgattc tgccgccatg
     7441 atcctagtta agaacccaac ccacctaaat ggtattataa tcaccagcaa catgtttggt
     7501 gatatcatct ccgatgaagc ctccgttatc ccaggttcct tgggtttgtt gccatctgcg
     7561 tccttggcct ctttgccaga caagaacacc gcatttggtt tgtacgaacc atgccacggt
     7621 tctgctccag atttgccaaa gaataaggtc aaccctatcg ccactatctt gtctgctgca
     7681 atgatgttga aattgtcatt gaacttgcct gaagaaggta aggccattga agatgcagtt
     7741 aaaaaggttt tggatgcagg tatcagaact ggtgatttag gtggttccaa cagtaccacc
     7801 gaagtcggtg atgctgtcgc cgaagaagtt aagaaaatcc ttgcttaaaa agattctctt
     7861 tttttatgat atttgtacat aaactttata aatgaaattc ataatagaaa cgacacgaaa
     7921 ttacaaaatg gaatatgttc atagggtaga cgaaactata tacgcaatct acatacattt
     7981 atcaagaagg agaaaaagga ggatgtaaag gaatacaggt aagcaaattg atactaatgg
     8041 ctcaacgtga taaggaaaaa gaattgcact ttaacattaa tattgacaag gaggagggca
     8101 ccacacaaaa agttaggtgt aacagaaaat catgaaacta tgattcctaa tttatatatt
     8161 ggaggatttt ctctaaaaaa aaaaaaatac aacaaataaa aaacactcaa tgacctgacc
     8221 atttgatgga gtttaagtca atacc
//
