LOCUS       sequence-191145-        8199 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CMYA5 (aa 4039 – 4069) Leu allele (4063) in a GAL4 AD vector, used 
            for yeast-two hybrid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8199)
  AUTHORS   Hsiung A, Naya FJ, Chen X, Shiang R
  TITLE     A schizophrenia associated CMYA5 allele displays differential 
            binding with desmin.
  JOURNAL   J Psychiatr Res. 2019 Apr;111:8-15. doi: 
            10.1016/j.jpsychires.2019.01.007. Epub 2019 Jan 10.
  PUBMED    30658136
REFERENCE   2  (bases 1 to 8199)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Psychiatr
            Res."; date: "2019-04"; volume: "111:8-15. doi"; pages: " 
            10.1016/j.jpsychires.2019.01.007. Epub 2019 Jan 10"
COMMENT     Sequence Label: CMYA5T/pACT2
FEATURES             Location/Qualifiers
     source          1..8199
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    112..145
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    223..256
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    300..333
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    411..444
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     terminator      complement(465..652)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     573..597
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(1162..1188)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             complement(1168..1194)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(1207..1548)
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="activation domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 activation domain
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     CDS             complement(1564..1584)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        complement(1630..2031)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     primer_bind     complement(2060..2082)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    2168..2308
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     2223..2242
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(2323..2340)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2494..3082)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2574..2593)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3253..4044,4045..4113))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3876..3895
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4114..4218)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4483..5825
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    4815..4862
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        6302..6707
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             6708..7802
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
ORIGIN
        1 ttcttgaacc atttcccata atggtgaaag ttccctcaag aattttactc tgtcagaaac
       61 ggccttaacg acgtagtcga ccaattccga tcatattcaa taacccttaa tataacttcg
      121 tataatgtat gctatacgaa gttattaggt ctgaagagga gtttacgtcc agccaagcta
      181 gcttggctgc aggtcgagcg gccgcgatcc ggaaccctta atataacttc gtataatgta
      241 tgctatacga agttattagg tccctcgacc aattccgatc atattcaata acccttaata
      301 taacttcgta taatgtatgc tatacgaagt tattaggtct gaagaggagt ttacgtccag
      361 ccaagctagc ttggctgcag gtcgagcggc cgcgatccgg aacccttaat ataacttcgt
      421 ataatgtatg ctatacgaag ttattaggtc cctcgaccgg catgccggta gaggtgtggt
      481 caataagagc gacctcatgc tatacctgag aaagcaacct gacctacagg aaagagttac
      541 tcaagaataa gaattttcgt tttaaaacct aagagtcact ttaaaatttg tatacactta
      601 ttttttttat aacttattta ataataaaaa tcataaatca taagaaattc gcttatttag
      661 aagtgtcaac aacgtatcta ccaacgattt gacccttttc catcttttcg taaatttctg
      721 gcaaggtaga caagccgaca accttgattg gagacttgac caaacctctg gcgaagaagt
      781 ccaaagcttc tgaataagcc ctcgtaatat attttcatga agaatttagg tccaaaaaaa
      841 agatgggcat taattctagt catttaaaaa attctataga tcagaggtta catggccaag
      901 attgaaactt agaggagtat agttacataa aagaaggcaa aacgatgtat aaatgaaaga
      961 aattgagatg gtgcacgatg cacagttgaa gtgaacttgc ggggtttttc agtatctacg
     1021 attcatagat ctctcgagct cgaattcctt gtgccttaca gaatccaggg gctctatccc
     1081 caggtgcaaa gtacattttc caggtttctc cagggcaaag gcagggtgga caccctcatt
     1141 aaccatggcc atatgaccac ccaagctagc gtaatctgga acatcgtatg ggtaagccat
     1201 agagatctct ttttttgggt ttggtggggt atcttcatca tcgaatagat agttatatac
     1261 atcatccatt gtagtggtat taaacatccc tgtagtgatt ccaaacgcgt tatacgcagt
     1321 ttggtccgtc caaccaggtg acagtggttt tgaattatta ccatcatcaa ttttactagc
     1381 cgtgatttca ttattcatga agttatcatg aacgttagag gaggcaattg gttgtgaaag
     1441 cgcttgagaa tttgtttgag ttgttatgag gttcggaccg ttgctactgt tagtgaaagt
     1501 gaaggacaat gagctatcag caatattccc actttgatta aaattggcgg cggtacccaa
     1561 ttcgaccttt ctcttctttt ttggaggctc gggaattaat tccgctttat ccatctttgc
     1621 aaagcttgga gttgattgta tgcttggtat agcttgaaat attgtgcaga aaaagaaaca
     1681 aggaagaaag ggaacgagaa caatgacgag gaaacaaaag attaataatt gcaggtctat
     1741 ttatacttga tagcaagaca gcaaactttt ttttatttca aattcaagta actggaagga
     1801 aggccgtata ccgttgctca ttagagagta gtgtgcgtga atgaaggaag gaaaaagttt
     1861 cgtgtgcttc gagatacccc tcatcagctc tggaacaacg acatctgttg gtgctgtctt
     1921 tgtcgttaat tttttccttt agtgtcttcc atcatttttt tgtcattgcg gatatggtga
     1981 gacaacaacg ggggagagag aaaagaaaaa aaaagaaaag aagttgctgc ctcgcgcgtt
     2041 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
     2101 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
     2161 gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta
     2221 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag
     2281 atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct
     2341 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     2401 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     2461 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     2521 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     2581 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     2641 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     2701 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     2761 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     2821 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     2881 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
     2941 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     3001 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     3061 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     3121 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     3181 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     3241 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     3301 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     3361 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     3421 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     3481 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     3541 tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg
     3601 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     3661 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     3721 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     3781 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     3841 gcgaccgagt tgctcttgcc cggcgtcaac acgggataat accgcgccac atagcagaac
     3901 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     3961 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     4021 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     4081 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     4141 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     4201 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgagcttt gaagaaaaat
     4261 gcgccttatt caatctttgc tataaaaaat ggcccaaaat ctcacattgg aagacatttg
     4321 atgacctcat ttctttcaat gaagggccta acggagttga ctaatgttgt gggaaattgg
     4381 agcgataagc gtgcttctgc cgtggccagg acaacgtata ctcatcagat aacagcaata
     4441 cctgatcact acttcgcact agtttctcgg tactatgcat atgatccaat atcaaaggaa
     4501 atgatagcat tgaaggatga gactaatcca attgaggagt ggcagcatat agaacagcta
     4561 aagggtagtg ctgaaggaag catacgatac cccgcatgga atgggataat atcacaggag
     4621 gtactagact acctttcatc ctacataaat agacgcatat aagtacgcat ttaagcataa
     4681 acacgcacta tgccgttctt ctcatgtata tatatataca ggcaacacgc agatataggt
     4741 gcgacgtgaa cagtgagctg tatgtgcgca gctcgcgttg cattttcgga agcgctcgtt
     4801 ttcggaaacg ctttgaagtt cctattccga agttcctatt ctctagaaag tataggaact
     4861 tcagagcgct tttgaaaacc aaaagcgctc tgaagacgca ctttcaaaaa accaaaaacg
     4921 caccggactg taacgagcta ctaaaatatt gcgaataccg cttccacaaa cattgctcaa
     4981 aagtatctct ttgctatata tctctgtgct atatccctat ataacctacc catccacctt
     5041 tcgctccttg aacttgcatc taaactcgac ctctacattt tttatgttta tctctagtat
     5101 tactctttag acaaaaaaat tgtagtaaga actattcata gagtgaatcg aaaacaatac
     5161 gaaaatgtaa acatttccta tacgtagtat atagagacaa aatagaagaa accgttcata
     5221 attttctgac caatgaagaa tcatcaacgc tatcactttc tgttcacaaa gtatgcgcaa
     5281 tccacatcgg tatagaatat aatcggggat gcctttatct tgaaaaaatg cacccgcagc
     5341 ttcgctagta atcagtaaac gcgggaagtg gagtcaggct ttttttatgg aagagaaaat
     5401 agacaccaaa gtagccttct tctaacctta acggacctac agtgcaaaaa gttatcaaga
     5461 gactgcatta tagagcgcac aaaggagaaa aaaagtaatc taagatgctt tgttagaaaa
     5521 atagcgctct cgggatgcat ttttgtagaa caaaaaagaa gtatagattc tttgttggta
     5581 aaatagcgct ctcgcgttgc atttctgttc tgtaaaaatg cagctcagat tctttgtttg
     5641 aaaaattagc gctctcgcgt tgcatttttg ttttacaaaa atgaagcaca gattcttcgt
     5701 tggtaaaata gcgctttcgc gttgcatttc tgttctgtaa aaatgcagct cagattcttt
     5761 gtttgaaaaa ttagcgctct cgcgttgcat ttttgttcta caaaatgaag cacagatgct
     5821 tcgttaacaa agatatgcta ttgaagtgca agatggaaac gcagaaaatg aaccggggat
     5881 gcgacgtgca agattaccta tgcaatagat gcaatagttt ctccaggaac cgaaatacat
     5941 acattgtctt ccgtaaagcg ctagactata tattattata caggttcaaa tatactatct
     6001 gtttcaggga aaactcccag gttcggatgt tcaaaattca atgatgggta acaagtacga
     6061 tcgtaaatct gtaaaacagt ttgtcggata ttaggctgta tctcctcaaa gcgtattcga
     6121 atatcattga gaagctgcag cgtcacatcg gataataatg atggcagcca ttgtagaagt
     6181 gccttttgca tttctagtct ctttctcggt ctagctagtt ttactacatc gcgaagatag
     6241 aatcttagat cacactgcct ttgctgagct ggatcaatag agtaacaaaa gagtggtaag
     6301 gaactgtggg aatactcagg tatcgtaaga tgcaagagtt cgaatctctt agcaaccatt
     6361 atttttttcc tcaacataac gagaacacac aggggcgcta tcgcacagaa tcaaattcga
     6421 tgactggaaa ttttttgtta atttcagagg tcgcctgacg catatacctt tttcaactga
     6481 aaaattggga gaaaaaggaa aggtgagagc gccggaaccg gcttttcata tagaatagag
     6541 aagcgttcat gactaaatgc ttgcatcaca atacttgaag ttgacaatat tatttaagga
     6601 cctattgttt tttccaatag gtggttagca atcgtcttac tttctaactt ttcttacctt
     6661 ttacatttca gcaatatata tatatatttc aaggatatac cattctaatg tctgccccta
     6721 agaagatcgt cgttttgcca ggtgaccacg ttggtcaaga aatcacagcc gaagccatta
     6781 aggttcttaa agctatttct gatgttcgtt ccaatgtcaa gttcgatttc gaaaatcatt
     6841 taattggtgg tgctgctatc gatgctacag gtgttccact tccagatgag gcgctggaag
     6901 cctccaagaa ggctgatgcc gttttgttag gtgctgtggg tggtcctaaa tggggtacag
     6961 gtagtgttag acctgaacaa ggtttactaa aaatccgtaa agaacttcaa ttgtacgcca
     7021 acttaagacc atgtaacttt gcatccgact ctcttttaga cttatctcca atcaagccac
     7081 aatttgctaa aggtactgac ttcgttgttg tcagagaatt agtgggaggt atttactttg
     7141 gtaagagaaa ggaagacgat ggtgatggtg tcgcttggga tagtgaacaa tacaccgttc
     7201 cagaagtgca aagaatcaca agaatggccg ctttcatggc cctacaacat gagccaccat
     7261 tgcctatttg gtccttggat aaagctaatg ttttggcctc ttcaagatta tggagaaaaa
     7321 ctgtggagga aaccatcaag aacgaatttc ctacattgaa ggttcaacat caattgattg
     7381 attctgccgc catgatccta gttaagaacc caacccacct aaatggtatt ataatcacca
     7441 gcaacatgtt tggtgatatc atctccgatg aagcctccgt tatcccaggt tccttgggtt
     7501 tgttgccatc tgcgtccttg gcctctttgc cagacaagaa caccgcattt ggtttgtacg
     7561 aaccatgcca cggttctgct ccagatttgc caaagaataa ggtcaaccct atcgccacta
     7621 tcttgtctgc tgcaatgatg ttgaaattgt cattgaactt gcctgaagaa ggtaaggcca
     7681 ttgaagatgc agttaaaaag gttttggatg caggtatcag aactggtgat ttaggtggtt
     7741 ccaacagtac caccgaagtc ggtgatgctg tcgccgaaga agttaagaaa atccttgctt
     7801 aaaaagattc tcttttttta tgatatttgt acataaactt tataaatgaa attcataata
     7861 gaaacgacac gaaattacaa aatggaatat gttcataggg tagacgaaac tatatacgca
     7921 atctacatac atttatcaag aaggagaaaa aggaggatgt aaaggaatac aggtaagcaa
     7981 attgatacta atggctcaac gtgataagga aaaagaattg cactttaaca ttaatattga
     8041 caaggaggag ggcaccacac aaaaagttag gtgtaacaga aaatcatgaa actatgattc
     8101 ctaatttata tattggagga ttttctctaa aaaaaaaaaa atacaacaaa taaaaaacac
     8161 tcaatgacct gaccatttga tggagtttaa gtcaatacc
//
