LOCUS       sequence-19115-e         725 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid was designed for screening purposes and may contain 
            discrepancies relative to the current canonical GenBank version of 
            the gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, 
            Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW
  TITLE     Identification of poxvirus CD8+ T cell determinants to enable 
            rational design and characterization of smallpox vaccines.
  JOURNAL   J Exp Med. 2005 January 3; 201(1): 95–104.
  PUBMED    15623576
REFERENCE   2  (bases 1 to 725)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "2005-?-3"; volume: " 201(1)"; pages: " 95–104"
COMMENT     Sequence Label: DCT.VV.Orf32
FEATURES             Location/Qualifiers
     source          1..725
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             504..545
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             555..572
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(595..612)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    601..712
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 accgaattcc agctgaccac catgggagaa tgttcctaat gtatacttta atcctgtgtt
       61 tatagagccc acgtttaaac attctttatt aagtgtttat aaacacagat taatagtttt
      121 atttgaagta ttcgttgtat tcattctaat atatgtattt tttagatctg aattaaatat
      181 gttcttcatg cctaaacgaa aaatacccga tcctattgat agattacgac gtgctaatct
      241 agcgtgtgaa gacgataaat taatgatcta tggattacca tggatgacaa ctcaaacatc
      301 tgcgttatca ataaatagta aaccgatagt gtataaagat tgtgcaaagc ttttgcgatc
      361 aataaatgga tcacaaccag tatctcttaa cgatgttctt cgcagatgag caattcccgg
      421 ggatccgaat aaagggtcaa gacaattctg cagatatcca gcacagtggc ggccgctcga
      481 gtctagaggg cccgcggttc gaaggtaagc ctatccctaa ccctctcctc ggtctcgatt
      541 ctacgcgtac cggtcatcat caccatcacc attgagttta aacccgctga tcagcctcga
      601 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
      661 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
      721 tgagt
//
