LOCUS       sequence-191150-        4899 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express CMYA5 (aa 4039 – 4069) with a N-terminal 6x-His tag in 
            bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4899)
  AUTHORS   Hsiung A, Naya FJ, Chen X, Shiang R
  TITLE     A schizophrenia associated CMYA5 allele displays differential 
            binding with desmin.
  JOURNAL   J Psychiatr Res. 2019 Apr;111:8-15. doi: 
            10.1016/j.jpsychires.2019.01.007. Epub 2019 Jan 10.
  PUBMED    30658136
REFERENCE   2  (bases 1 to 4899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Psychiatr
            Res."; date: "2019-04"; volume: "111:8-15. doi"; pages: " 
            10.1016/j.jpsychires.2019.01.007. Epub 2019 Jan 10"
COMMENT     Sequence Label: CMYA5/pDEST17
FEATURES             Location/Qualifiers
     source          1..4899
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     90..109
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        90..108
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             140..162
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             182..199
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    209..233
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(410..426)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(411..427)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     protein_bind    complement(437..461)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(514..532)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      528..575
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(941..946,947..963,964..969))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(996..1014)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1082..1186
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1187..1255,1256..2047)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1405..1424)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2218..2806
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2707..2726
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2960..2977
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2992..3131
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3057..3076)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3217..3239
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(3233..3424)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     join(4886..4899,1..6)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt
       61 agaggatcga gatctcgatc ccgcgaaatt aatacgactc actataggga gaccacaacg
      121 gtttccctct agaaataatt ttgtttaact ttaagaagga gatatacata tgtcgtacta
      181 ccatcaccat caccatcacc tcgaatcaac aagtttgtac aaaaaagcag gctctttaaa
      241 ggaaccgggc tgcaggaatt cgattgaatt ccttgtgcct tacagaatcc ccatggttaa
      301 tgagggtgtc caccctgcct ttgccctgga gaaacctgga aaatgtactt tgcacctggg
      361 gatagagccc ccggattctg taaggcacaa ggaattcaat caagcttatc gataccgtcg
      421 acctcgagat atctagaccc agctttcttg tacaaagtgg ttgattcgag gctgctaaca
      481 aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc
      541 ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat
      601 atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa
      661 gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat
      721 agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg
      781 gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca
      841 gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt
      901 gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata
      961 agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata
     1021 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
     1081 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     1141 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     1201 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     1261 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     1321 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     1381 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg
     1441 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     1501 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     1561 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     1621 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     1681 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc
     1741 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     1801 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     1861 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     1921 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     1981 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     2041 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     2101 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     2161 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     2221 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     2281 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     2341 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     2401 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     2461 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     2521 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     2581 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     2641 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     2701 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     2761 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     2821 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     2881 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     2941 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg
     3001 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc aatggtgcac tctcagtaca
     3061 atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg
     3121 tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     3181 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     3241 tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt
     3301 gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa
     3361 gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg
     3421 tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac
     3481 gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt
     3541 gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc
     3601 aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg
     3661 cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg
     3721 aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc
     3781 agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc
     3841 gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc
     3901 caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt
     3961 tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc
     4021 ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg
     4081 gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat
     4141 ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg
     4201 tccaatgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg
     4261 gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga
     4321 agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac
     4381 gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt
     4441 ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag
     4501 cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag
     4561 cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac
     4621 gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat
     4681 cggtcgatcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt
     4741 tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca
     4801 gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga
     4861 agtggcgagc ccgatcttcc ccatcggtga tgtcggcga
//
