LOCUS       sequence-19116-e         746 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid was designed for screening purposes and may contain 
            discrepancies relative to the current canonical GenBank version of 
            the gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, 
            Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW
  TITLE     Identification of poxvirus CD8+ T cell determinants to enable 
            rational design and characterization of smallpox vaccines.
  JOURNAL   J Exp Med. 2005 January 3; 201(1): 95–104.
  PUBMED    15623576
REFERENCE   2  (bases 1 to 746)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "2005-?-3"; volume: " 201(1)"; pages: " 95–104"
COMMENT     Sequence Label: DCT.VV.Orf33
FEATURES             Location/Qualifiers
     source          1..746
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             589..630
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             640..657
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(680..697)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 cagtaccctt caccgaattc cagctgacca ccatggatca caaccagtat ctcttaacga
       61 tgttcttcgc agatgatgat tcatttttta agtatttggc tagtcaagat gatgaatctt
      121 cattatctga tatattgcaa atcactcaat atctagactt tctgttatta ttattgatcc
      181 aatcaaaaaa taaattagaa gccgtgggtc attgttatga atctctttca gaggaataca
      241 gacaattgac aaaattcaca gactttcaag attttaaaaa actgtttaac aaggtcccta
      301 ttgttacaga tggaagggtc aaacttaata aaggatattt gttcgacttt gtgattagtt
      361 tgatgcgatt caaaaaagaa tcctctctag ctaccaccgc aatagatcct gttagataca
      421 tagatcctcg tcgcaatatc gcattttcta acgtgatgga tatattaaag tcgaataaag
      481 tgaacaataa ttaagcaatt cccggggatc cgaataaagg gtcaagacaa ttctgcagat
      541 atccagcaca gtggcggccg ctcgagtcta gagggcccgc ggttcgaagg taagcctatc
      601 cctaaccctc tcctcggtct cgattctacg cgtaccggtc atcatcacca tcaccattga
      661 gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
      721 ccctcccccg tgccttcctt gaccct
//
