LOCUS       sequence-191201-       12006 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Deletion from NL4-3 nt 5582-6199 encompassing Vif and Vpr coding 
            sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12006)
  AUTHORS   McCauley SM, Kim K, Nowosielska A, Dauphin A, Yurkovetskiy L, Diehl 
            WE, Luban J
  TITLE     Intron-containing RNA from the HIV-1 provirus activates type I 
            interferon and inflammatory cytokines.
  JOURNAL   Nat Commun. 2018 Dec 13;9(1):5305. doi: 10.1038/s41467-018-07753-2.
  PUBMED    30546110
REFERENCE   2  (bases 1 to 12006)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-12-13"; volume: "9(1):5305. doi"; pages: " 
            10.1038/s41467-018-07753-2"
COMMENT     Sequence Label: pNL4-3 envFS eGFP Delta-vif/vpr
FEATURES             Location/Qualifiers
     source          1..12006
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      7..435
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(94..113)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     304..325
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     LTR             833..1090
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     CDS             1246..2748
                     /codon_start=1
                     /gene="gag"
                     /product="gag protein from human immunodeficiency virus 1"
                     /label=HIV-1 gag
                     /translation="MGARASVLSGGELDKWEKIRLRPGGKKQYKLKHIVWASRELERFA
                     VNPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTIAVLYCVHQRIDVKDTKEALDKI
                     EEEQNKSKKKAQQAAADTGNNSQVSQNYPIVQNLQGQMVHQAISPRTLNAWVKVVEEKA
                     FSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRLHPVHAGP
                     IAPGQMREPRGSDIAGTTSTLQEQIGWMTHNPPIPVGEIYKRWIILGLNKIVRMYSPTS
                     ILDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTILKALGPG
                     ATLEEMMTACQGVGGPGHKARVLAEAMSQVTNPATIMIQKGNFRNQRKTVKCFNCGKEG
                     HIAKNCRAPRKKGCWKCGKEGHQMKDCTERQANFLGKIWPSHKGRPGNFLQSRPEPTAP
                     PEESFRFGEETTTPSQKQEPIDKELYPLASLRSLFGSDPSSQ"
     CDS             2541..5552
                     /codon_start=1
                     /gene="pol"
                     /product="pol protein from human immunodeficiency virus 1"
                     /label=HIV-1 pol
                     /translation="FFREDLAFPQGKAREFSSEQTRANSPTRRELQVWGRDNNSLSEAG
                     ADRQGTVSFSFPQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMNLPGRWKPKMIG
                     GIGGFIKVGQYDQILIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNFPISPIETV
                     PVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPVFAIKKKD
                     STKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKQKKSVTVLDVGDAYFSVPLDKDFRK
                     YTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQCSMTKILEPFRKQNPDIVIYQYMD
                     DLYVGSDLEIGQHRTKIEELRQHLLRWGFTTPDKKHQKEPPFLWMGYELHPDKWTVQPI
                     VLPEKDSWTVNDIQKLVGKLNWASQIYAGIKVRQLCKLLRGTKALTEVVPLTEEAELEL
                     AENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMKGAHT
                     NDVKQLTEAVQKIATESIVIWGKTPKFKLPIQKETWEAWWTEYWQATWIPEWEFVNTPP
                     LVKLWYQLEKEPIIGAETFYVDGAANRETKLGKAGYVTDRGRQKVVPLTDTTNQKTELQ
                     AIHLALQDSGLEVNIVTDSQYALGIIQAQPDKSESELVSQIIEQLIKKEKVYLAWVPAH
                     KGIGGNEQVDGLVSAGIRKVLFLDGIDKAQEEHEKYHSNWRAMASDFNLPPVVAKEIVA
                     SCDKCQLKGEAMHGQVDCSPGIWQLDCTHLEGKVILVAVHVASGYIEAEVIPAETGQET
                     AYFLLKLAGRWPVKTVHTDNGSNFTSTTVKAACWWAGIKQEFGIPYNPQSQGVIESMNK
                     ELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGERIVDIIATDIQTKELQK
                     QITKIQNFRVYYRDSRDPVWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQ
                     MAGDDCVASRQDED"
     misc_feature    5237..5354
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    7599..7832
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8017..8061
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             8355..8381
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             join(8648..8650,8651..9364)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(8648..8650,8651..9361)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(8690..8711)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8951..8970)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9298..9319
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             9553..10186
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(10262..10850)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10342..10361)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11021..11812,11813..11881))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11644..11663
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11882..11986)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
       61 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
      121 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
      181 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
      241 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
      301 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
      361 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
      421 aatttaacgc gaattttaac aaaatatttt aattaatgga agggctaatt tggtcccaaa
      481 aaagacaaga gatccttgat ctgtggatct accacacaca aggctacttc cctgattggc
      541 agaactacac accagggcca gggatcagat atccactgac ctttggatgg tgcttcaagt
      601 tagtaccagt tgaaccagag caagtagaag aggccaaata aggagagaag aacagcttgt
      661 tacaccctat gagccagcat gggatggagg acccggaggg agaagtatta gtgtggaagt
      721 ttgacagcct cctagcattt cgtcacatgg cccgagagct gcatccggag tactacaaag
      781 actgctgaca tcgagctttc tacaagggac tttccgctgg ggactttcca gggaggtgtg
      841 gcctgggcgg gactggggag tggcgagccc tcagatgcta catataagca gctgcttttt
      901 gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
      961 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgctcaaagt agtgtgtgcc
     1021 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     1081 atctctagca gtggcgcccg aacagggact tgaaagcgaa agtaaagcca gaggagatct
     1141 ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg
     1201 tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg
     1261 tcggtattaa gcgggggaga attagataaa tgggaaaaaa ttcggttaag gccaggggga
     1321 aagaaacaat ataaactaaa acatatagta tgggcaagca gggagctaga acgattcgca
     1381 gttaatcctg gccttttaga gacatcagaa ggctgtagac aaatactggg acagctacaa
     1441 ccatcccttc agacaggatc agaagaactt agatcattat ataatacaat agcagtcctc
     1501 tattgtgtgc atcaaaggat agatgtaaaa gacaccaagg aagccttaga taagatagag
     1561 gaagagcaaa acaaaagtaa gaaaaaggca cagcaagcag cagctgacac aggaaacaac
     1621 agccaggtca gccaaaatta ccctatagtg cagaacctcc aggggcaaat ggtacatcag
     1681 gccatatcac ctagaacttt aaatgcatgg gtaaaagtag tagaagagaa ggctttcagc
     1741 ccagaagtaa tacccatgtt ttcagcatta tcagaaggag ccaccccaca agatttaaat
     1801 accatgctaa acacagtggg gggacatcaa gcagccatgc aaatgttaaa agagaccatc
     1861 aatgaggaag ctgcagaatg ggatagattg catccagtgc atgcagggcc tattgcacca
     1921 ggccagatga gagaaccaag gggaagtgac atagcaggaa ctactagtac ccttcaggaa
     1981 caaataggat ggatgacaca taatccacct atcccagtag gagaaatcta taaaagatgg
     2041 ataatcctgg gattaaataa aatagtaaga atgtatagcc ctaccagcat tctggacata
     2101 agacaaggac caaaggaacc ctttagagac tatgtagacc gattctataa aactctaaga
     2161 gccgagcaag cttcacaaga ggtaaaaaat tggatgacag aaaccttgtt ggtccaaaat
     2221 gcgaacccag attgtaagac tattttaaaa gcattgggac caggagcgac actagaagaa
     2281 atgatgacag catgtcaggg agtgggggga cccggccata aagcaagagt tttggctgaa
     2341 gcaatgagcc aagtaacaaa tccagctacc ataatgatac agaaaggcaa ttttaggaac
     2401 caaagaaaga ctgttaagtg tttcaattgt ggcaaagaag ggcacatagc caaaaattgc
     2461 agggccccta ggaaaaaggg ctgttggaaa tgtggaaagg aaggacacca aatgaaagat
     2521 tgtactgaga gacaggctaa ttttttaggg aagatctggc cttcccacaa gggaaggcca
     2581 gggaattttc ttcagagcag accagagcca acagccccac cagaagagag cttcaggttt
     2641 ggggaagaga caacaactcc ctctcagaag caggagccga tagacaagga actgtatcct
     2701 ttagcttccc tcagatcact ctttggcagc gacccctcgt cacaataaag ataggggggc
     2761 aattaaagga agctctatta gatacaggag cagatgatac agtattagaa gaaatgaatt
     2821 tgccaggaag atggaaacca aaaatgatag ggggaattgg aggttttatc aaagtaggac
     2881 agtatgatca gatactcata gaaatctgcg gacataaagc tataggtaca gtattagtag
     2941 gacctacacc tgtcaacata attggaagaa atctgttgac tcagattggc tgcactttaa
     3001 attttcccat tagtcctatt gagactgtac cagtaaaatt aaagccagga atggatggcc
     3061 caaaagttaa acaatggcca ttgacagaag aaaaaataaa agcattagta gaaatttgta
     3121 cagaaatgga aaaggaagga aaaatttcaa aaattgggcc tgaaaatcca tacaatactc
     3181 cagtatttgc cataaagaaa aaagacagta ctaaatggag aaaattagta gatttcagag
     3241 aacttaataa gagaactcaa gatttctggg aagttcaatt aggaatacca catcctgcag
     3301 ggttaaaaca gaaaaaatca gtaacagtac tggatgtggg cgatgcatat ttttcagttc
     3361 ccttagataa agacttcagg aagtatactg catttaccat acctagtata aacaatgaga
     3421 caccagggat tagatatcag tacaatgtgc ttccacaggg atggaaagga tcaccagcaa
     3481 tattccagtg tagcatgaca aaaatcttag agccttttag aaaacaaaat ccagacatag
     3541 tcatctatca atacatggat gatttgtatg taggatctga cttagaaata gggcagcata
     3601 gaacaaaaat agaggaactg agacaacatc tgttgaggtg gggatttacc acaccagaca
     3661 aaaaacatca gaaagaacct ccattccttt ggatgggtta tgaactccat cctgataaat
     3721 ggacagtaca gcctatagtg ctgccagaaa aggacagctg gactgtcaat gacatacaga
     3781 aattagtggg aaaattgaat tgggcaagtc agatttatgc agggattaaa gtaaggcaat
     3841 tatgtaaact tcttagggga accaaagcac taacagaagt agtaccacta acagaagaag
     3901 cagagctaga actggcagaa aacagggaga ttctaaaaga accggtacat ggagtgtatt
     3961 atgacccatc aaaagactta atagcagaaa tacagaagca ggggcaaggc caatggacat
     4021 atcaaattta tcaagagcca tttaaaaatc tgaaaacagg aaaatatgca agaatgaagg
     4081 gtgcccacac taatgatgtg aaacaattaa cagaggcagt acaaaaaata gccacagaaa
     4141 gcatagtaat atggggaaag actcctaaat ttaaattacc catacaaaag gaaacatggg
     4201 aagcatggtg gacagagtat tggcaagcca cctggattcc tgagtgggag tttgtcaata
     4261 cccctccctt agtgaagtta tggtaccagt tagagaaaga acccataata ggagcagaaa
     4321 ctttctatgt agatggggca gccaataggg aaactaaatt aggaaaagca ggatatgtaa
     4381 ctgacagagg aagacaaaaa gttgtccccc taacggacac aacaaatcag aagactgagt
     4441 tacaagcaat tcatctagct ttgcaggatt cgggattaga agtaaacata gtgacagact
     4501 cacaatatgc attgggaatc attcaagcac aaccagataa gagtgaatca gagttagtca
     4561 gtcaaataat agagcagtta ataaaaaagg aaaaagtcta cctggcatgg gtaccagcac
     4621 acaaaggaat tggaggaaat gaacaagtag atgggttggt cagtgctgga atcaggaaag
     4681 tactattttt agatggaata gataaggccc aagaagaaca tgagaaatat cacagtaatt
     4741 ggagagcaat ggctagtgat tttaacctac cacctgtagt agcaaaagaa atagtagcca
     4801 gctgtgataa atgtcagcta aaaggggaag ccatgcatgg acaagtagac tgtagcccag
     4861 gaatatggca gctagattgt acacatttag aaggaaaagt tatcttggta gcagttcatg
     4921 tagccagtgg atatatagaa gcagaagtaa ttccagcaga gacagggcaa gaaacagcat
     4981 acttcctctt aaaattagca ggaagatggc cagtaaaaac agtacataca gacaatggca
     5041 gcaatttcac cagtactaca gttaaggccg cctgttggtg ggcggggatc aagcaggaat
     5101 ttggcattcc ctacaatccc caaagtcaag gagtaataga atctatgaat aaagaattaa
     5161 agaaaattat aggacaggta agagatcagg ctgaacatct taagacagca gtacaaatgg
     5221 cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa
     5281 gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa
     5341 aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccagtttgg aaaggaccag
     5401 caaagctcct ctggaaaggt gaaggggcag tagtaataca agataatagt gacataaaag
     5461 tagtgccaag aagaaaagca aagatcatca gggattatgg aaaacagatg gcaggtgatg
     5521 attgtgtggc aagtagacag gatgaggatt aacacatgga aaagattagt aaaacaccat
     5581 aaattctgca acaactgctg tttatccatt tcagaattgg gtgtcgacat agcagaatag
     5641 gcgttactcg acagaggaga gcaagaaatg gagccagtag atcctagact agagccctgg
     5701 aagcatccag gaagtcagcc taaaactgct tgtaccaatt gctattgtaa aaagtgttgc
     5761 tttcattgcc aagtttgttt catgacaaaa gccttaggca tctcctatgg caggaagaag
     5821 cggagacagc gacgaagagc tcatcagaac agtcagactc atcaagcttc tctatcaaag
     5881 cagtaagtag tacatgtaat gcaacctata atagtagcaa tagtagcatt agtagtagca
     5941 ataataatag caatagttgt gtggtccata gtaatcatag aatataggaa aatattaaga
     6001 caaagaaaaa tagacaggtt aattgataga ctaatagaaa gagcagaaga cagtggcaat
     6061 gagagtgaag gagaagtatc agcacttgtg gagatggggg tggaaatggg gcaccatgct
     6121 ccttgggata ttgatgatct gtagtgctac agaaaaattg tgggtcaccg tctattatgg
     6181 ggtacctgtg tggaaggaag caaccaccac tctattttgt gcatcagatg ctaaagcata
     6241 tatgatacag aggtacataa tgtttgggcc acacatgcct gtgtacccac agaccccaac
     6301 ccacaagaag tagtattggt aaatgtgaca gaaaatttta acatgtggaa aaatgacatg
     6361 gtagaacaga tgcatgagga tataatcagt ttatgggatc aaagcctaaa gccatgtgta
     6421 aaattaaccc cactctgtgt tagtttaaag tgcactgatt tgaagaatga tactaatacc
     6481 aatagtagta gcgggagaat gataatggag aaaggagaga taaaaaactg ctctttcaat
     6541 atcagcacaa gcataagaga taaggtgcag aaagaatatg cattctttta taaacttgat
     6601 atagtaccaa tagataatac cagctatagg ttgataagtt gtaacacctc agtcattaca
     6661 caggcctgtc caaaggtatc ctttgagcca attcccatac attattgtgc cccggctggt
     6721 tttgcgattc taaaatgtaa taataagacg ttcaatggaa caggaccatg tacaaatgtc
     6781 agcacagtac aatgtacaca tggaatcagg ccagtagtat caactcaact gctgttaaat
     6841 ggcagtctag cagaagaaga tgtagtaatt agatctgcca atttcacaga caatgctaaa
     6901 accataatag tacagctgaa cacatctgta gaaattaatt gtacaagacc caacaacaat
     6961 acaagaaaaa gtatccgtat ccagagggga ccagggagag catttgttac aataggaaaa
     7021 ataggaaata tgagacaagc acattgtaac attagtagag caaaatggaa tgccacttta
     7081 aaacagatag ctagcaaatt aagagaacaa tttggaaata ataaaacaat aatctttaag
     7141 caatcctcag gaggggaccc agaaattgta acgcacagtt ttaattgtgg aggggaattt
     7201 ttctactgta attcaacaca actgtttaat agtacttggt ttaatagtac ttggagtact
     7261 gaagggtcaa ataacactga aggaagtgac acaatcacac tcccatgcag aataaaacaa
     7321 tttataaaca tgtggcagga agtaggaaaa gcaatgtatg cccctcccat cagtggacaa
     7381 attagatgtt catcaaatat tactgggctg ctattaacaa gagatggtgg taataacaac
     7441 aatgggtccg agatcttcag acctggagga ggcgatatga gggacaattg gagaagtgaa
     7501 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     7561 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     7621 ttgggagcag caggaagcac tatgggcgca cggtcaatga cgctgacggt acaggccaga
     7681 caattattgt ctgatatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     7741 cagcatctgt tgcaactcac agtctggggc atcaaacagc tccaggcaag aatcctggct
     7801 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     7861 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     7921 tggaataaca tgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     7981 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     8041 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     8101 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     8161 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     8221 atcccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
     8281 gacagatcca ttcgattagt gaacggatcc ttagcactta tctgggacga tctgcggagc
     8341 ctgtgcctct tcagctacca ccgcttgaga gacttactct tgattgtaac gaggattgtg
     8401 gaacttctgg gacgcagggg gtgggaagcc ctcaaatatt ggtggaatct cctacagtat
     8461 tggagtcagg aactaaagaa tagtgctgtt aacttgctca atgccacagc catagcagta
     8521 gctgagggga cagatagggt tatagaagta ttacaagcag cttatagagc tattcgccac
     8581 atacctagaa gaataagaca gggcttggaa aggattttgc tataagatgg gtggcgcggc
     8641 cgctctggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
     8701 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
     8761 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
     8821 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
     8881 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
     8941 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     9001 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     9061 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
     9121 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     9181 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     9241 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     9301 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     9361 gtaactcgag cctagaaaaa catggagcaa tcacaagtag caatacagca gctaacaatg
     9421 ctgcttgtgc ctggctagaa gcacaagagg aggaagaggt gggttttcca gtcacacctc
     9481 aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     9541 aaaagggggg actggaaggg ctaattcact cccaaagaag acaagatatc cttgatctgt
     9601 ggatctacca cacacaaggc tacttccctg attggcagaa ctacacacca gggccagggg
     9661 tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag ccagataagg
     9721 tagaagaggc caataaagga gagaacacca gcttgttaca ccctgtgagc ctgcatggaa
     9781 tggatgaccc tgagagagaa gtgttagagt ggaggtttga cagccgccta gcatttcatc
     9841 acgtggcccg agagctgcat ccggagtact tcaagaactg ctgacatcga gcttgctaca
     9901 agggactttc cgctggggac tttccaggga ggcgtggcct gggcgggact ggggagtggc
     9961 gagccctcag atgctgcata taagcagctg ctttttgcct gtactgggtc tctctggtta
    10021 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
    10081 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
    10141 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcaggcc ggcccaattg
    10201 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
    10261 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
    10321 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
    10381 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
    10441 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
    10501 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
    10561 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
    10621 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
    10681 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
    10741 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
    10801 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
    10861 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
    10921 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
    10981 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
    11041 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
    11101 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
    11161 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
    11221 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
    11281 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
    11341 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
    11401 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
    11461 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
    11521 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
    11581 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
    11641 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
    11701 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
    11761 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
    11821 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
    11881 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
    11941 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
    12001 aagtgc
//
