LOCUS       sequence-191207-       13660 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  HIV-1 delta Rev was cloned into pSC101 and modified to include an 
            RTEm26CTE element in order to utilize the NXF1 RNA export pathway. 
            The RRE was also mutated..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13660)
  AUTHORS   McCauley SM, Kim K, Nowosielska A, Dauphin A, Yurkovetskiy L, Diehl 
            WE, Luban J
  TITLE     Intron-containing RNA from the HIV-1 provirus activates type I 
            interferon and inflammatory cytokines.
  JOURNAL   Nat Commun. 2018 Dec 13;9(1):5305. doi: 10.1038/s41467-018-07753-2.
  PUBMED    30546110
REFERENCE   2  (bases 1 to 13660)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-12-13"; volume: "9(1):5305. doi"; pages: " 
            10.1038/s41467-018-07753-2"
COMMENT     Sequence Label: pSC101 NL4-3 envFS Delta-rev Delta-RRE RTEm26CTE
FEATURES             Location/Qualifiers
     source          1..13660
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      9..80
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     LTR             477..734
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     CDS             890..2392
                     /codon_start=1
                     /gene="gag"
                     /product="gag protein from human immunodeficiency virus 1"
                     /label=HIV-1 gag
                     /translation="MGARASVLSGGELDKWEKIRLRPGGKKQYKLKHIVWASRELERFA
                     VNPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTIAVLYCVHQRIDVKDTKEALDKI
                     EEEQNKSKKKAQQAAADTGNNSQVSQNYPIVQNLQGQMVHQAISPRTLNAWVKVVEEKA
                     FSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRLHPVHAGP
                     IAPGQMREPRGSDIAGTTSTLQEQIGWMTHNPPIPVGEIYKRWIILGLNKIVRMYSPTS
                     ILDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTILKALGPG
                     ATLEEMMTACQGVGGPGHKARVLAEAMSQVTNPATIMIQKGNFRNQRKTVKCFNCGKEG
                     HIAKNCRAPRKKGCWKCGKEGHQMKDCTERQANFLGKIWPSHKGRPGNFLQSRPEPTAP
                     PEESFRFGEETTTPSQKQEPIDKELYPLASLRSLFGSDPSSQ"
     CDS             2185..5196
                     /codon_start=1
                     /gene="pol"
                     /product="pol protein from human immunodeficiency virus 1"
                     /label=HIV-1 pol
                     /translation="FFREDLAFPQGKAREFSSEQTRANSPTRRELQVWGRDNNSLSEAG
                     ADRQGTVSFSFPQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMNLPGRWKPKMIG
                     GIGGFIKVGQYDQILIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNFPISPIETV
                     PVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPVFAIKKKD
                     STKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKQKKSVTVLDVGDAYFSVPLDKDFRK
                     YTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQCSMTKILEPFRKQNPDIVIYQYMD
                     DLYVGSDLEIGQHRTKIEELRQHLLRWGFTTPDKKHQKEPPFLWMGYELHPDKWTVQPI
                     VLPEKDSWTVNDIQKLVGKLNWASQIYAGIKVRQLCKLLRGTKALTEVVPLTEEAELEL
                     AENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMKGAHT
                     NDVKQLTEAVQKIATESIVIWGKTPKFKLPIQKETWEAWWTEYWQATWIPEWEFVNTPP
                     LVKLWYQLEKEPIIGAETFYVDGAANRETKLGKAGYVTDRGRQKVVPLTDTTNQKTELQ
                     AIHLALQDSGLEVNIVTDSQYALGIIQAQPDKSESELVSQIIEQLIKKEKVYLAWVPAH
                     KGIGGNEQVDGLVSAGIRKVLFLDGIDKAQEEHEKYHSNWRAMASDFNLPPVVAKEIVA
                     SCDKCQLKGEAMHGQVDCSPGIWQLDCTHLEGKVILVAVHVASGYIEAEVIPAETGQET
                     AYFLLKLAGRWPVKTVHTDNGSNFTSTTVKAACWWAGIKQEFGIPYNPQSQGVIESMNK
                     ELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGERIVDIIATDIQTKELQK
                     QITKIQNFRVYYRDSRDPVWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQ
                     MAGDDCVASRQDED"
     misc_feature    4881..4998
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             8279..8323
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             8617..8643
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     LTR             9618..10251
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     terminator      complement(10318..10345)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(10881..11831)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      11879..12101
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(12593..12687)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(12718..13512)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(12829..12848)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     13439..13458
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
       61 gtttgtcggt gaacgctctc gcgcgcacgc gtttaattaa tggaagggct aatttggtcc
      121 caaaaaagac aagagatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat
      181 tggcagaact acacaccagg gccagggatc agatatccac tgacctttgg atggtgcttc
      241 aagttagtac cagttgaacc agagcaagta gaagaggcca aataaggaga gaagaacagc
      301 ttgttacacc ctatgagcca gcatgggatg gaggacccgg agggagaagt attagtgtgg
      361 aagtttgaca gcctcctagc atttcgtcac atggcccgag agctgcatcc ggagtactac
      421 aaagactgct gacatcgagc tttctacaag ggactttccg ctggggactt tccagggagg
      481 tgtggcctgg gcgggactgg ggagtggcga gccctcagat gctacatata agcagctgct
      541 ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
      601 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgctca aagtagtgtg
      661 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
      721 gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagtaaa gccagaggag
      781 atctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga
      841 ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag
      901 agcgtcggta ttaagcgggg gagaattaga taaatgggaa aaaattcggt taaggccagg
      961 gggaaagaaa caatataaac taaaacatat agtatgggca agcagggagc tagaacgatt
     1021 cgcagttaat cctggccttt tagagacatc agaaggctgt agacaaatac tgggacagct
     1081 acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata caatagcagt
     1141 cctctattgt gtgcatcaaa ggatagatgt aaaagacacc aaggaagcct tagataagat
     1201 agaggaagag caaaacaaaa gtaagaaaaa ggcacagcaa gcagcagctg acacaggaaa
     1261 caacagccag gtcagccaaa attaccctat agtgcagaac ctccaggggc aaatggtaca
     1321 tcaggccata tcacctagaa ctttaaatgc atgggtaaaa gtagtagaag agaaggcttt
     1381 cagcccagaa gtaataccca tgttttcagc attatcagaa ggagccaccc cacaagattt
     1441 aaataccatg ctaaacacag tggggggaca tcaagcagcc atgcaaatgt taaaagagac
     1501 catcaatgag gaagctgcag aatgggatag attgcatcca gtgcatgcag ggcctattgc
     1561 accaggccag atgagagaac caaggggaag tgacatagca ggaactacta gtacccttca
     1621 ggaacaaata ggatggatga cacataatcc acctatccca gtaggagaaa tctataaaag
     1681 atggataatc ctgggattaa ataaaatagt aagaatgtat agccctacca gcattctgga
     1741 cataagacaa ggaccaaagg aaccctttag agactatgta gaccgattct ataaaactct
     1801 aagagccgag caagcttcac aagaggtaaa aaattggatg acagaaacct tgttggtcca
     1861 aaatgcgaac ccagattgta agactatttt aaaagcattg ggaccaggag cgacactaga
     1921 agaaatgatg acagcatgtc agggagtggg gggacccggc cataaagcaa gagttttggc
     1981 tgaagcaatg agccaagtaa caaatccagc taccataatg atacagaaag gcaattttag
     2041 gaaccaaaga aagactgtta agtgtttcaa ttgtggcaaa gaagggcaca tagccaaaaa
     2101 ttgcagggcc cctaggaaaa agggctgttg gaaatgtgga aaggaaggac accaaatgaa
     2161 agattgtact gagagacagg ctaatttttt agggaagatc tggccttccc acaagggaag
     2221 gccagggaat tttcttcaga gcagaccaga gccaacagcc ccaccagaag agagcttcag
     2281 gtttggggaa gagacaacaa ctccctctca gaagcaggag ccgatagaca aggaactgta
     2341 tcctttagct tccctcagat cactctttgg cagcgacccc tcgtcacaat aaagataggg
     2401 gggcaattaa aggaagctct attagataca ggagcagatg atacagtatt agaagaaatg
     2461 aatttgccag gaagatggaa accaaaaatg atagggggaa ttggaggttt tatcaaagta
     2521 ggacagtatg atcagatact catagaaatc tgcggacata aagctatagg tacagtatta
     2581 gtaggaccta cacctgtcaa cataattgga agaaatctgt tgactcagat tggctgcact
     2641 ttaaattttc ccattagtcc tattgagact gtaccagtaa aattaaagcc aggaatggat
     2701 ggcccaaaag ttaaacaatg gccattgaca gaagaaaaaa taaaagcatt agtagaaatt
     2761 tgtacagaaa tggaaaagga aggaaaaatt tcaaaaattg ggcctgaaaa tccatacaat
     2821 actccagtat ttgccataaa gaaaaaagac agtactaaat ggagaaaatt agtagatttc
     2881 agagaactta ataagagaac tcaagatttc tgggaagttc aattaggaat accacatcct
     2941 gcagggttaa aacagaaaaa atcagtaaca gtactggatg tgggcgatgc atatttttca
     3001 gttcccttag ataaagactt caggaagtat actgcattta ccatacctag tataaacaat
     3061 gagacaccag ggattagata tcagtacaat gtgcttccac agggatggaa aggatcacca
     3121 gcaatattcc agtgtagcat gacaaaaatc ttagagcctt ttagaaaaca aaatccagac
     3181 atagtcatct atcaatacat ggatgatttg tatgtaggat ctgacttaga aatagggcag
     3241 catagaacaa aaatagagga actgagacaa catctgttga ggtggggatt taccacacca
     3301 gacaaaaaac atcagaaaga acctccattc ctttggatgg gttatgaact ccatcctgat
     3361 aaatggacag tacagcctat agtgctgcca gaaaaggaca gctggactgt caatgacata
     3421 cagaaattag tgggaaaatt gaattgggca agtcagattt atgcagggat taaagtaagg
     3481 caattatgta aacttcttag gggaaccaaa gcactaacag aagtagtacc actaacagaa
     3541 gaagcagagc tagaactggc agaaaacagg gagattctaa aagaaccggt acatggagtg
     3601 tattatgacc catcaaaaga cttaatagca gaaatacaga agcaggggca aggccaatgg
     3661 acatatcaaa tttatcaaga gccatttaaa aatctgaaaa caggaaaata tgcaagaatg
     3721 aagggtgccc acactaatga tgtgaaacaa ttaacagagg cagtacaaaa aatagccaca
     3781 gaaagcatag taatatgggg aaagactcct aaatttaaat tacccataca aaaggaaaca
     3841 tgggaagcat ggtggacaga gtattggcaa gccacctgga ttcctgagtg ggagtttgtc
     3901 aatacccctc ccttagtgaa gttatggtac cagttagaga aagaacccat aataggagca
     3961 gaaactttct atgtagatgg ggcagccaat agggaaacta aattaggaaa agcaggatat
     4021 gtaactgaca gaggaagaca aaaagttgtc cccctaacgg acacaacaaa tcagaagact
     4081 gagttacaag caattcatct agctttgcag gattcgggat tagaagtaaa catagtgaca
     4141 gactcacaat atgcattggg aatcattcaa gcacaaccag ataagagtga atcagagtta
     4201 gtcagtcaaa taatagagca gttaataaaa aaggaaaaag tctacctggc atgggtacca
     4261 gcacacaaag gaattggagg aaatgaacaa gtagatgggt tggtcagtgc tggaatcagg
     4321 aaagtactat ttttagatgg aatagataag gcccaagaag aacatgagaa atatcacagt
     4381 aattggagag caatggctag tgattttaac ctaccacctg tagtagcaaa agaaatagta
     4441 gccagctgtg ataaatgtca gctaaaaggg gaagccatgc atggacaagt agactgtagc
     4501 ccaggaatat ggcagctaga ttgtacacat ttagaaggaa aagttatctt ggtagcagtt
     4561 catgtagcca gtggatatat agaagcagaa gtaattccag cagagacagg gcaagaaaca
     4621 gcatacttcc tcttaaaatt agcaggaaga tggccagtaa aaacagtaca tacagacaat
     4681 ggcagcaatt tcaccagtac tacagttaag gccgcctgtt ggtgggcggg gatcaagcag
     4741 gaatttggca ttccctacaa tccccaaagt caaggagtaa tagaatctat gaataaagaa
     4801 ttaaagaaaa ttataggaca ggtaagagat caggctgaac atcttaagac agcagtacaa
     4861 atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg
     4921 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt
     4981 acaaaaattc aaaattttcg ggtttattac agggacagca gagatccagt ttggaaagga
     5041 ccagcaaagc tcctctggaa aggtgaaggg gcagtagtaa tacaagataa tagtgacata
     5101 aaagtagtgc caagaagaaa agcaaagatc atcagggatt atggaaaaca gatggcaggt
     5161 gatgattgtg tggcaagtag acaggatgag gattaacaca tggaaaagat tagtaaaaca
     5221 ccatatgtat atttcaagga aagctaagga ctggttttat agacatcact atgaaagtac
     5281 taatccaaaa ataagttcag aagtacacat cccactaggg gatgctaaat tagtaataac
     5341 aacatattgg ggtctgcata caggagaaag agactggcat ttgggtcagg gagtctccat
     5401 agaatggagg aaaaagagat atagcacaca agtagaccct gacctagcag accaactaat
     5461 tcatctgcac tattttgatt gtttttcaga atctgctata agaaatacca tattaggacg
     5521 tatagttagt cctaggtgtg aatatcaagc aggacataac aaggtaggat ctctacagta
     5581 cttggcacta gcagcattaa taaaaccaaa acagataaag ccacctttgc ctagtgttag
     5641 gaaactgaca gaggacagat ggaacaagcc ccagaagacc aagggccaca gagggagcca
     5701 tacaatgaat ggacactaga gcttttagag gaacttaaga gtgaagctgt tagacatttt
     5761 cctaggatat ggctccataa cttaggacaa catatctatg aaacttacgg ggatacttgg
     5821 gcaggagtgg aagccataat aagaattctg caacaactgc tgtttatcca tttcagaatt
     5881 gggtgtcgac atagcagaat aggcgttact cgacagagga gagcaagaaa tggagccagt
     5941 agatcctaga ctagagccct ggaagcatcc aggaagtcag cctaaaactg cttgtaccaa
     6001 ttgctattgt aaaaagtgtt gctttcattg ccaagtttgt ttcatgacaa aagccttagg
     6061 catctcctac ggcaggaaga agcggagaca gcgacgaaga gctcatcaga acagtcagac
     6121 tcatcaggct tctctaagca agcagtaagt agtacatgta atgcaaccta taatagtagc
     6181 aatagtagca ttagtagtag caataataat agcaatagtt gtgtggtcca tagtaatcat
     6241 agaatatagg aaaatattaa gacaaagaaa aatagacagg ttaattgata gactaataga
     6301 aagagcagaa gacagtggca atgagagtga aggagaagta tcagcacttg tggagatggg
     6361 ggtggaaatg gggcaccatg ctccttggga tattgatgat ctgtagtgct acagaaaaat
     6421 tgtgggtcac cgtctattat ggggtacctg tgtggaagga agcaaccacc actctatttt
     6481 gtgcatcaga tgctaaagca tatatgatac agaggtacat aatgtttggg ccacacatgc
     6541 ctgtgtaccc acagacccca acccacaaga agtagtattg gtaaatgtga cagaaaattt
     6601 taacatgtgg aaaaatgaca tggtagaaca gatgcatgag gatataatca gtttatggga
     6661 tcaaagccta aagccatgtg taaaattaac cccactctgt gttagtttaa agtgcactga
     6721 tttgaagaat gatactaata ccaatagtag tagcgggaga atgataatgg agaaaggaga
     6781 gataaaaaac tgctctttca atatcagcac aagcataaga gataaggtgc agaaagaata
     6841 tgcattcttt tataaacttg atatagtacc aatagataat accagctata ggttgataag
     6901 ttgtaacacc tcagtcatta cacaggcctg tccaaaggta tcctttgagc caattcccat
     6961 acattattgt gccccggctg gttttgcgat tctaaaatgt aataataaga cgttcaatgg
     7021 aacaggacca tgtacaaatg tcagcacagt acaatgtaca catggaatca ggccagtagt
     7081 atcaactcaa ctgctgttaa atggcagtct agcagaagaa gatgtagtaa ttagatctgc
     7141 caatttcaca gacaatgcta aaaccataat agtacagctg aacacatctg tagaaattaa
     7201 ttgtacaaga cccaacaaca atacaagaaa aagtatccgt atccagaggg gaccagggag
     7261 agcatttgtt acaataggaa aaataggaaa tatgagacaa gcacattgta acattagtag
     7321 agcaaaatgg aatgccactt taaaacagat agctagcaaa ttaagagaac aatttggaaa
     7381 taataaaaca ataatcttta agcaatcctc aggaggggac ccagaaattg taacgcacag
     7441 ttttaattgt ggaggggaat ttttctactg taattcaaca caactgttta atagtacttg
     7501 gtttaatagt acttggagta ctgaagggtc aaataacact gaaggaagtg acacaatcac
     7561 actcccatgc agaataaaac aatttataaa catgtggcag gaagtaggaa aagcaatgta
     7621 tgcccctccc atcagtggac aaattagatg ttcatcaaat attactgggc tgctattaac
     7681 aagagatggt ggtaataaca acaatgggtc cgagatcttc agacctggag gaggcgatat
     7741 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg
     7801 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat
     7861 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
     7921 gacccttacc gtgcaagctc gccagctgct gtctggtata gtgcagcagc agaacaacct
     7981 cctccgcgca atcgaagccc agcagcatct gttgcaactc acagtctggg gcatcaaaca
     8041 gctccaggca agaatccttg ccgttgagag gtatctgaaa gaccagcaac ttcttgggat
     8101 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
     8161 taataaatct ctggaacaga tttggaataa catgacctgg atggagtggg acagagaaat
     8221 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
     8281 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
     8341 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
     8401 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
     8461 atcgtttcag acccacctcc caatcccgag gggacccgac aggcccgaag gaatagaaga
     8521 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ccttagcact
     8581 tatctgggac gatctgcgga gcctgtgcct cttcagctac caccgcttga gagacttact
     8641 cttgattgta acgaggattg tggaacttct gggacgcagg gggtgggaag ccctcaaata
     8701 ttggtggaat ctcctacagt attggagtca ggaactaaag aatagtgctg ttaacttgct
     8761 caatgccaca gccatagcag tagctgaggg gacagatagg gttatagaag tattacaagc
     8821 agcttataga gctattcgcc acatacctag aagaataaga cagggcttgg aaaggatttt
     8881 gctataagat gggtggcaag tggtcaaaaa gtagtgtgat tggatggcct gctgtaaggg
     8941 aaagaatgag acgagctgag ccagcagcag atggggtggg agcagtatct cgagtccgtg
     9001 gggtgcgagg ctaagcactg cacagaggat agcttgctgt tggcatcctg tggaaggcac
     9061 gtctgattgc atgaaggttc agtgtcctag ttcccttccc ccaggaaaaa cgacacggga
     9121 gctggccaag acctctctgg gtgatgagcc taagggatgg ttttgtgtag ggcccctatg
     9181 cttggcggct ggggatcaga cctctacctt cacccatgag gtatcgatac cgcggggatc
     9241 ctctagagta gaccacctcc cctgcgagct aagctggaca gccaatgacg ggtaagagag
     9301 tgacattttt cactaaccta agacaggagg gccgtcagag ctactgccta atccaaagac
     9361 gggtaaaagt gataaaaatg tatcactcca acctaagaca ggcgcagctt ccgagggatt
     9421 tggagctcct cgagacctag aaaaacatgg agcaatcaca agtagcaata cagcagctaa
     9481 caatgctgct tgtgcctggc tagaagcaca agaggaggaa gaggtgggtt ttccagtcac
     9541 acctcaggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
     9601 aaaagaaaag gggggactgg aagggctaat tcactcccaa agaagacaag atatccttga
     9661 tctgtggatc taccacacac aaggctactt ccctgattgg cagaactaca caccagggcc
     9721 aggggtcaga tatccactga cctttggatg gtgctacaag ctagtaccag ttgagccaga
     9781 taaggtagaa gaggccaata aaggagagaa caccagcttg ttacaccctg tgagcctgca
     9841 tggaatggat gaccctgaga gagaagtgtt agagtggagg tttgacagcc gcctagcatt
     9901 tcatcacgtg gcccgagagc tgcatccgga gtacttcaag aactgctgac atcgagcttg
     9961 ctacaaggga ctttccgctg gggactttcc agggaggcgt ggcctgggcg ggactgggga
    10021 gtggcgagcc ctcagatgct gcatataagc agctgctttt tgcctgtact gggtctctct
    10081 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
    10141 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
    10201 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc aggaaacaaa
    10261 taataaaaaa gccggattaa taatctggct ttttatattc tctctctagt atataaacgc
    10321 agaaaggccc acccgaaggt gagccagtgt gaatcgattc ctagggtacg ggttttgctg
    10381 cccgcaaacg ggctgttctg gtgttgctag tttgttatca gaatcgcaga tccggcttca
    10441 ggtttgccgg ctgaaagcgc tatttcttcc agaattgcca tgattttttc cccacgggag
    10501 gcgtcactgg ctcccgtgtt gtcggcagct ttgattcgat aagcagcatc gcctgtttca
    10561 ggctgtctat gtgtgactgt tgagctgtaa caagttgtct caggtgttca atttcatgtt
    10621 ctagttgctt tgttttactg gtttcacctg ttctattagg tgttacatgc tgttcatctg
    10681 ttacattgtc gatctgttca tggtgaacag ctttaaatgc accaaaaact cgtaaaagct
    10741 ctgatgtatc tatctttttt acaccgtttt catctgtgca tatggacagt tttccctttg
    10801 atatctaacg gtgaacagtt gttctacttt tgtttgttag tcttgatgct tcactgatag
    10861 atacaagagc cataagaacc tcagatcctt ccgtatttag ccagtatgtt ctctagtgtg
    10921 gttcgttgtt tttgcgtgag ccatgagaac gaaccattga gatcatgctt actttgcatg
    10981 tcactcaaaa attttgcctc aaaactggtg agctgaattt ttgcagttaa agcatcgtgt
    11041 agtgtttttc ttagtccgtt acgtaggtag gaatctgatg taatggttgt tggtattttg
    11101 tcaccattca tttttatctg gttgttctca agttcggtta cgagatccat ttgtctatct
    11161 agttcaactt ggaaaatcaa cgtatcagtc gggcggcctc gcttatcaac caccaatttc
    11221 atattgctgt aagtgtttaa atctttactt attggtttca aaacccattg gttaagcctt
    11281 ttaaactcat ggtagttatt ttcaagcatt aacatgaact taaattcatc aaggctaatc
    11341 tctatatttg ccttgtgagt tttcttttgt gttagttctt ttaataacca ctcataaatc
    11401 ctcatagagt atttgttttc aaaagactta acatgttcca gattatattt tatgaatttt
    11461 tttaactgga aaagataagg caatatctct tcactaaaaa ctaattctaa tttttcgctt
    11521 gagaacttgg catagtttgt ccactggaaa atctcaaagc ctttaaccaa aggattcctg
    11581 atttccacag ttctcgtcat cagctctctg gttgctttag ctaatacacc ataagcattt
    11641 tccctactga tgttcatcat ctgagcgtat tggttataag tgaacgatac cgtccgttct
    11701 ttccttgtag ggttttcaat cgtggggttg agtagtgcca cacagcataa aattagcttg
    11761 gtttcatgct ccgttaagtc atagcgacta atcgctagtt catttgcttt gaaaacaact
    11821 aattcagaca tacatctcaa ttggtctagg tgattttaat cactatacca attgagatgg
    11881 gctagtcaat gataattact agtccttttc ctttgagttg tgggtatctg taaattctgc
    11941 tagacctttg ctggaaaact tgtaaattct gctagaccct ctgtaaattc cgctagacct
    12001 ttgtgtgttt tttttgttta tattcaagtg gttataattt atagaataaa gaaagaataa
    12061 aaaaagataa aaagaataga tcccagccct gtgtataact cactacttta gtcagttccg
    12121 cagtattaca aaaggatgtc gcaaacgctg tttgctcctc tacaaaacag accttaaaac
    12181 cctaaaggct taagtagcac cctcgcaagc tcgggcaaat cgctgaatat tccttttgtc
    12241 tccgaccatc aggcacctga gtcgctgtct ttttcgtgac attcagttcg ctgcgctcac
    12301 ggctctggca gtgaatgggg gtaaatggca ctacaggcgc cttttatgga ttcatgcaag
    12361 gaaactaccc ataatacaag aaaagcccgt cacgggcttc tcagggcgtt ttatggcggg
    12421 tctgctatgt ggtgctatct gactttttgc tgttcagcag ttcctgccct ctgattttcc
    12481 agtctgacca cttcggatta tcccgtgaca ggtcattcag actggctaat gcacccagta
    12541 aggcagcggt atcatcaaca ggcttacccg tcttactgtc cctagtgctt ggattctcac
    12601 caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa atccagatgg agttctgagg
    12661 tcattactgg atctatcaac aggagtccaa gcgagctctc gaaccccaga gtcccgctca
    12721 gaagaactcg tcaagaaggc gatagaaggc gatgcgctgc gaatcgggag cggcgatacc
    12781 gtaaagcacg aggaagcggt cagcccattc gccgccaagc tcttcagcaa tatcacgggt
    12841 agccaacgct atgtcctgat agcggtccgc cacacccagc cggccacagt cgatgaatcc
    12901 agaaaagcgg ccattttcca ccatgatatt cggcaagcag gcatcgccat gggtcacgac
    12961 gagatcctcg ccgtcgggca tgcgcgcctt gagcctggcg aacagttcgg ctggcgcgag
    13021 cccctgatgc tcttcgtcca gatcatcctg atcgacaaga ccggcttcca tccgagtacg
    13081 tgctcgctcg atgcgatgtt tcgcttggtg gtcgaatggg caggtagccg gatcaagcgt
    13141 atgcagccgc cgcattgcat cagccatgat ggatactttc tcggcaggag caaggtgaga
    13201 tgacaggaga tcctgccccg gcacttcgcc caatagcagc cagtcccttc ccgcttcagt
    13261 gacaacgtcg agcacagctg cgcaaggaac gcccgtcgtg gccagccacg atagccgcgc
    13321 tgcctcgtcc tgcagttcat tcagggcacc ggacaggtcg gtcttgacaa aaagaaccgg
    13381 gcgcccctgc gctgacagcc ggaacacggc ggcatcagag cagccgattg tctgttgtgc
    13441 ccagtcatag ccgaatagcc tctccaccca agcggccgga gaacctgcgt gcaatccatc
    13501 ttgttcaatc atgcgaaacg atcctcatcc tgtctcttga tcagatcttg atcccctgcg
    13561 ccatcagatc cttggcggca agaaagccat ccagtttact ttgcagggct tcccaacctt
    13621 accagagggc gccccagctg gcaattccga cgtcaccggt
//
