LOCUS       sequence-191210-        6786 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  An AAV vector that expresses nuclear localized iRFP under control of
            the c-fos promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6786)
  AUTHORS   Richie CT, Whitaker LR, Whitaker KW, Necarsulmer J, Baldwin HA, 
            Zhang Y, Fortuno L, Hinkle JJ, Koivula P, Henderson MJ, Sun W, Wang 
            K, Smith JC, Pickel J, Ji N, Hope BT, Harvey BK
  TITLE     Near-infrared fluorescent protein iRFP713 as a reporter protein for 
            optogenetic vectors, a transgenic Cre-reporter rat, and other 
            neuronal studies.
  JOURNAL   J Neurosci Methods. 2017 Jun 1;284:1-14. doi: 
            10.1016/j.jneumeth.2017.03.020. Epub 2017 Apr 2.
  PUBMED    28380331
REFERENCE   2  (bases 1 to 6786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Neurosci 
            Methods."; date: "2017-06-1"; volume: "284:1-14. doi"; pages: " 
            10.1016/j.jneumeth.2017.03.020. Epub 2017 Apr 2"
COMMENT     Sequence Label: pOTTC588 - pAAV c-fos Nuc-iRFP
FEATURES             Location/Qualifiers
     source          1..6786
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   12..141
                     /label=AAV2 ITR
     CDS             complement(856..867)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             1833..2780
                     /codon_start=1
                     /product="phytochrome-based near-infrared fluorescent 
                     protein, originally termed iRFP (Filonov et al., 2011)"
                     /label=iRFP713
                     /note="name was changed to iRFP713 (Shcherbakova and 
                     Verkhusha, 2013)"
                     /translation="MAEGSVARQPDLLTCDDEPIHIPGAIQPHGLLLALAADMTIVAGS
                     DNLPELTGLAIGALIGRSAADVFDSETHNRLTIALAEPGAAVGAPITVGFTMRKDAGFI
                     GSWHRHDQLIFLELEPPQRDVAEPQAFFRRTNSAIRRLQAAETLESACAAAAQEVRKIT
                     GFDRVMIYRFASDFSGEVIAEDRCAEVESKLGLHYPASTVPAQARRLYTINPVRIIPDI
                     NYRPVPVTPDLNPVTGRPIDLSFAILRSVSPVHLEFMRNIGMHGTMSISILRGERLWGL
                     IVCHHRTPYYVDLDGRQACELVAQVLAWQIGVMEE"
     CDS             2814..2834
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2838..2858
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2862..2882
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    2913..3501
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2966..2986)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3384..3395)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3533..4009
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3732..3751)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   4049..4189
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4049..4178
                     /label=AAV2 ITR
     rep_origin      4264..4719
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4351..4370)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4561..4582
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4736..4755)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4855..4877
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4915..4933)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5001..5105
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5106..5174,5175..5966)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5324..5343)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6137..6725
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6626..6645
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtaagct ttcctttagg aacagaggct
      181 tcgagccttt aaggctgcgt acttgcttct cctaatacca gagactcaaa aaaaaaaaaa
      241 aagttccaga ttgctggaca atgacccggg tctcatccct tgaccctggg aaccgggtcc
      301 acattgaatc aggtgcgaat gttcgctcgc cttctctgcc tttcccgcct cccctccccc
      361 ggccgcggcc ccggttcccc ccctgcgctg caccctcaga gttggctgca gccggcgagc
      421 tgttcccgtc aatccctccc tcctttacac aggatgtcca tattaggaca tctgcgtcag
      481 caggtttcca cggccggtcc ctgttgttct ggggggggga ccatctccga aatcctacac
      541 gcggaaggtc taggagaccc cctaagatcc caaatgtgaa cactcatagg tgaaagatgt
      601 atgccaagac gggggttgaa agcctggggc gtagagttga cgacagagcg cccgcagagg
      661 gccttggggc gcgcttcccc ccccttccag ttccgcccag tgacgtagga agtccatcca
      721 ttcacagcgc ttctataaag gcgccagctg aggcgcctac tactccaacc gcgactgcag
      781 cgagcaactg agaagactgg atagagccgg cggttccgcg aacgagcagt gaccgcgctc
      841 ccacccagct ctgctctgca gctcccacca gtgtctaccc ctggacccct tgccgggctt
      901 tccccaaact tcgaccatga tgttctcggg tttcaacgcc gactacgagg cgtcatcctc
      961 ccgctgcagt agcgcctccc cggccgggga cagcctttcc tactaccatt ccccagccga
     1021 ctccttctcc agcatgggct ctcctgtcaa cacacaggtg agtttggctt tgtgtagccg
     1081 ccaggtccgc gctgagggtc gccgtggagg agacactggg gtgtgactcg caggggcggg
     1141 ggggtcttcc tttttcgctc tggagggaga ctggcgcggt cagagcagcc ttagcctggg
     1201 aacccaggac ttgtctgagc gcgtgcacac ttgtcatagt aagacttagt gaccccttcc
     1261 cgcgcggcag gtttattctg agtggcctgc ctgcattctt ctctcggccg acttgtttct
     1321 gagatcagcc ggggccaaca agtctcgagc aaagagtcgc taactagagt ttgggaggcg
     1381 gcaaaccgcg gcaatccccc ctcccggggc agcctggagc agggaggagg gaggagggag
     1441 gagggtgctg cgggcgggtg tgtaaggcag tttcattgat aaaaagcgag ttcattctgg
     1501 agactccgga gcagcgcctg cgtcagcgca gacgtcaggg atatttataa caaaccccct
     1561 ttcgagcgag tgatgccgaa gggataacgg gaacgcagca gtaggatgga ggggaaaggc
     1621 tgcgctgcgg aattcaaggg aggatattgg gagagctttt atctccgatg aggtgcatac
     1681 aggaagacat aagcagtctc tgaccggaat gcttctctct ccctgcttca tgcgacacta
     1741 gggccacttg ctccacctgt gtctggaacc tcctcgctca cctccgcttt cctctttttg
     1801 ttttgtttca ggacttttgc gcagatctct ccatggctga aggatccgtc gccaggcagc
     1861 ctgacctctt gacctgcgac gatgagccga tccatatccc cggtgccatc caaccgcatg
     1921 gactgctgct cgccctcgcc gccgacatga cgatcgttgc cggcagcgac aaccttcccg
     1981 aactcaccgg actggcgatc ggcgccctga tcggccgctc tgcggccgat gtcttcgact
     2041 cggagacgca caaccgtctg acgatcgcct tggccgagcc cggggcggcc gtcggagcac
     2101 cgatcactgt cggcttcacg atgcgaaagg acgcaggctt catcggctcc tggcatcgcc
     2161 atgatcagct catcttcctc gagctcgagc ctccccagcg ggacgtcgcc gagccgcagg
     2221 cgttcttccg ccgcaccaac agcgccatcc gccgcctgca ggccgccgaa accttggaaa
     2281 gcgcctgcgc cgccgcggcg caagaggtgc ggaagattac cggcttcgat cgggtgatga
     2341 tctatcgctt cgcctccgac ttcagcggcg aagtgatcgc agaggatcgg tgcgccgagg
     2401 tcgagtcaaa actaggcctg cactatcctg cctcaaccgt gccggcgcag gcccgtcggc
     2461 tctataccat caacccggta cggatcattc ccgatatcaa ttatcggccg gtgccggtca
     2521 ccccagacct caatccggtc accgggcggc cgattgatct tagcttcgcc atcctgcgca
     2581 gcgtctcgcc cgtccatctg gaattcatgc gcaacatagg catgcacggc acgatgtcga
     2641 tctcgatttt gcgcggcgag cgactgtggg gattgatcgt ttgccatcac cgaacgccgt
     2701 actacgtcga tctcgatggc cgccaagcct gcgagctagt cgcccaggtt ctggcctggc
     2761 agatcggcgt gatggaagag ttgtacaagt ccggcctgcg cagccgcgcc gacccaaaaa
     2821 agaagcgcaa ggtagacccc aagaagaagc gcaaggtaga tccaaaaaag aagagaaagg
     2881 tatgatagga attcgatatc aagcttatcg ataatcaacc tctggattac aaaatttgtg
     2941 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     3001 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     3061 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     3121 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     3181 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     3241 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     3301 cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg
     3361 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     3421 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     3481 ccctttgggc cgcctccccg catcgatacc gagcgctgct cgagagatct acgggtggca
     3541 tccctgtgac ccctccccag tgcctctcct ggccctggaa gttgccactc cagtgcccac
     3601 cagccttgtc ctaataaaat taagttgcat cattttgtct gactaggtgt ccttctataa
     3661 tattatgggg tggagggggg tggtatggag caaggggcaa gttgggaaga caacctgtag
     3721 ggcctgcggg gtctattggg aaccaagctg gagtgcagtg gcacaatctt ggctcactgc
     3781 aatctccgcc tcctgggttc aagcgattct cctgcctcag cctcccgagt tgttgggatt
     3841 ccaggcatgc atgaccaggc tcagctaatt tttgtttttt tggtagagac ggggtttcac
     3901 catattggcc aggctggtct ccaactccta atctcaggtg atctacccac cttggcctcc
     3961 caaattgctg ggattacagg cgtgaaccac tgctcccttc cctgtccttc tgattttgta
     4021 ggtaaccacg tgcggaccga gcggccgcag gaacccctag tgatggagtt ggccactccc
     4081 tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc
     4141 tttgcccggg cggcctcagt gagcgagcga gcgcgcagct gcctgcaggg gcgcctgatg
     4201 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacgtca aagcaaccat
     4261 agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     4321 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     4381 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     4441 ttagtgcttt acggcacctc gaccccaaaa aacttgattt gggtgatggt tcacgtagtg
     4501 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     4561 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcgggctat tcttttgatt
     4621 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     4681 ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt atggtgcact ctcagtacaa
     4741 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc
     4801 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
     4861 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg
     4921 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
     4981 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     5041 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     5101 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     5161 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     5221 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     5281 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     5341 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     5401 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     5461 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     5521 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     5581 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     5641 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     5701 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     5761 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     5821 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     5881 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     5941 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     6001 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     6061 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     6121 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     6181 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     6241 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     6301 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     6361 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     6421 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     6481 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     6541 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     6601 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     6661 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     6721 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     6781 acatgt
//
