LOCUS       sequence-191215-       10771 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of BRAF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10771)
  AUTHORS   Rosenbluh J, Mercer J, Shrestha Y, Oliver R, Tamayo P, Doench JG, 
            Tirosh I, Piccioni F, Hartenian E, Horn H, Fagbami L, Root DE, Jaffe
            J, Lage K, Boehm JS, Hahn WC
  TITLE     Genetic and Proteomic Interrogation of Lower Confidence Candidate 
            Genes Reveals Signaling Networks in beta-Catenin-Active Cancers.
  JOURNAL   Cell Syst. 2016 Sep 28;3(3):302-316.e4. doi: 
            10.1016/j.cels.2016.09.001.
  PUBMED    27684187
REFERENCE   2  (bases 1 to 10771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2016.09"; journalName: "Cell Syst"; date: 
            "2016-09-28- 28"; volume: "3"; issue: "3"; pages: "302-316.e4"
COMMENT     Sequence Label: BRAF_pLX307
FEATURES             Location/Qualifiers
     source          1..10771
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        98..1276
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          329..1267
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1224..1244
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1296..1320
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      1321..1330
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(3622..3646)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3654..3695
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3806..4394
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3859..3879)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4277..4288)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4466..4699
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4777..4898
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4814..4833)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4868..4887
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4938..5073
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5093..5112)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5094..5112)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5122..5139)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5122..5138)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5131..5153)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5280..5735
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5367..5386)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5577..5598
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5761..5865
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5866..5934,5935..6726)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6084..6103)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6897..7485
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7386..7405
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7639..7656
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7773..7794
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7809..7814,7815..7832,7833..7839)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7847..7863
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7852..7874
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7871..7887
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7871..7887
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7906..7926
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7908..7926
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7954..8180
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8181..8361
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8408..8533
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9026..9259
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9444..9488
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9719..9929
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9741..9937
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9788..9923
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9969..10571
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9969..9988)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10465..10485
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10614..10731
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctcgaggc ctgcaggtgc aaagatggat aaagttttaa acagagagga atctttgcag
       61 ctaatggacc ttctaggtct tgaaaggagt gggaattggc tccggtgccc gtcagtgggc
      121 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
      181 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
      241 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
      301 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
      361 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt
      421 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta
      481 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt
      541 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta
      601 aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg
      661 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc
      721 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg
      781 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc
      841 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc
      901 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc
      961 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga
     1021 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac
     1081 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt
     1141 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt
     1201 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc
     1261 atttcaggtg tcgtgaggct agcatcgatt gatcaacaag tttgtacaaa aaagttggca
     1321 ccatggcggc gctgagcggt ggcggtggtg gcggcgcgga gccgggccag gctctgttca
     1381 acggggacat ggagcccgag gccggcgccg gcgccggcgc cgcggcctct tcggctgcgg
     1441 accctgccat tccggaggag gtgtggaata tcaaacaaat gattaagttg acacaggaac
     1501 atatagaggc cctattggac aaatttggtg gggagcataa tccaccatca atatatctgg
     1561 aggcctatga agaatacacc agcaagctag atgcactcca acaaagagaa caacagttat
     1621 tggaatctct ggggaacgga actgattttt ctgtttctag ctctgcatca atggataccg
     1681 ttacatcttc ttcctcttct agcctttcag tgctaccttc atctctttca gtttttcaaa
     1741 atcccacaga tgtggcacgg agcaacccca agtcaccaca aaaacctatc gttagagtct
     1801 tcctgcccaa caaacagagg acagtggtac ctgcaaggtg tggagttaca gtccgagaca
     1861 gtctaaagaa agcactgatg atgagaggtc taatcccaga gtgctgtgct gtttacagaa
     1921 ttcaggatgg agagaagaaa ccaattggtt gggacactga tatttcctgg cttactggag
     1981 aagaattgca tgtggaagtg ttggagaatg ttccacttac aacacacaac tttgtacgaa
     2041 aaacgttttt caccttagca ttttgtgact tttgtcgaaa gctgcttttc cagggtttcc
     2101 gctgtcaaac atgtggttat aaatttcacc agcgttgtag tacagaagtt ccactgatgt
     2161 gtgttaatta tgaccaactt gatttgctgt ttgtctccaa gttctttgaa caccacccaa
     2221 taccacagga agaggcgtcc ttagcagaga ctgccctaac atctggatca tccccttccg
     2281 cacccgcctc ggactctatt gggccccaaa ttctcaccag tccgtctcct tcaaaatcca
     2341 ttccaattcc acagcccttc cgaccagcag atgaagatca tcgaaatcaa tttgggcaac
     2401 gagaccgatc ctcatcagct cccaatgtgc atataaacac aatagaacct gtcaatattg
     2461 atgacttgat tagagaccaa ggatttcgtg gtgatggagg atcaaccaca ggtttgtctg
     2521 ctaccccccc tgcctcatta cctggctcac taactaacgt gaaagcctta cagaaatctc
     2581 caggacctca gcgagaaagg aagtcatctt catcctcaga agacaggaat cgaatgaaaa
     2641 cacttggtag acgggactcg agtgatgatt gggagattcc tgatgggcag attacagtgg
     2701 gacaaagaat tggatctgga tcatttggaa cagtctacaa gggaaagtgg catggtgatg
     2761 tggcagtgaa aatgttgaat gtgacagcac ctacacctca gcagttacaa gccttcaaaa
     2821 atgaagtagg agtactcagg aaaacacgac atgtgaatat cctactcttc atgggctatt
     2881 ccacaaagcc acaactggct attgttaccc agtggtgtga gggctccagc ttgtatcacc
     2941 atctccatat cattgagacc aaatttgaga tgatcaaact tatagatatt gcacgacaga
     3001 ctgcacaggg catggattac ttacacgcca agtcaatcat ccacagagac ctcaagagta
     3061 ataatatatt tcttcatgaa gacctcacag taaaaatagg tgattttggt ctagctacag
     3121 tgaaatctcg atggagtggg tcccatcagt ttgaacagtt gtctggatcc attttgtgga
     3181 tggcaccaga agtcatcaga atgcaagata aaaatccata cagctttcag tcagatgtat
     3241 atgcatttgg aattgttctg tatgaattga tgactggaca gttaccttat tcaaacatca
     3301 acaacaggga ccagataatt tttatggtgg gacgaggata cctgtctcca gatctcagta
     3361 aggtacggag taactgtcca aaagccatga agagattaat ggcagagtgc ctcaaaaaga
     3421 aaagagatga gagaccactc tttccccaaa ttctcgcctc tattgagctg ctggcccgct
     3481 cattgccaaa aattcaccgc agtgcatcag aaccctcctt gaatcgggct ggtttccaaa
     3541 cagaggattt tagtctatat gcttgtgctt ctccaaaaac acccatccag gcagggggat
     3601 atggtgcgtt tcctgtccac ttgccaactt tcttgtacaa agtggttgat atcggtaagc
     3661 ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgaactagt ccgtaacttg
     3721 aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgatctg aattagggtt
     3781 gatcccccgc acgcgttaag tcgacaatca acctctggat tacaaaattt gtgaaagatt
     3841 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     3901 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     3961 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     4021 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     4081 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     4141 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     4201 atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     4261 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     4321 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     4381 ggccgcctcc ccgcgtcgac tttaagacca atgacttaca aggcagctgt agatcttagc
     4441 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat
     4501 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     4561 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     4621 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     4681 gtgtggaaaa tctctagcag tacgtatagt agttcatgtc atcttattat tcagtattta
     4741 taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat
     4801 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     4861 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc
     4921 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     4981 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     5041 tccagaagta gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag
     5101 tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
     5161 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
     5221 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga
     5281 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     5341 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     5401 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     5461 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     5521 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     5581 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     5641 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     5701 cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg
     5761 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     5821 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     5881 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     5941 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     6001 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     6061 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     6121 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     6181 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     6241 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     6301 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     6361 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     6421 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     6481 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     6541 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     6601 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     6661 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     6721 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     6781 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     6841 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     6901 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     6961 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     7021 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     7081 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     7141 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     7201 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     7261 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     7321 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     7381 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     7441 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     7501 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     7561 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     7621 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     7681 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     7741 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     7801 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     7861 caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac
     7921 taaagggaac aaaagctgga gctgcaagct taatgtagtc ttatgcaata ctcttgtagt
     7981 cttgcaacat ggtaacgatg agttagcaac atgccttaca aggagagaaa aagcaccgtg
     8041 catgccgatt ggtggaagta aggtggtacg atcgtgcctt attaggaagg caacagacgg
     8101 gtctgacatg gattggacga accactgaat tgccgcattg cagagatatt gtatttaagt
     8161 gcctagctcg atacataaac gggtctctct ggttagacca gatctgagcc tgggagctct
     8221 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     8281 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     8341 cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc
     8401 agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg
     8461 gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg
     8521 gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt
     8581 aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct
     8641 agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact
     8701 gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac
     8761 agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt
     8821 agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga
     8881 tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata
     8941 aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc
     9001 agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag
     9061 gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg
     9121 gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc
     9181 aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc
     9241 taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg
     9301 ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga
     9361 cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg
     9421 aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg
     9481 caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa
     9541 tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata
     9601 gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac
     9661 ccatgcattg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     9721 aggcagaagt atgcaaagca tgcgtctcaa ttagtcagca accatagtcc cgcccctaac
     9781 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     9841 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     9901 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctttctagag gtaccaccgg
     9961 agcttaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca
    10021 gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg
    10081 atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg
    10141 ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgccgtggcg gtctggacca
    10201 cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt
    10261 tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc
    10321 ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg
    10381 gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg
    10441 ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg
    10501 tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg
    10561 gtgccagtta acgaattcta gatcttgaga caaatggcag tattcatcca caattttaaa
    10621 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
    10681 gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat
    10741 tacagggaca gcagagatcc actttggcgc c
//
