LOCUS       sequence-191216-       10704 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of JUP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10704)
  AUTHORS   Rosenbluh J, Mercer J, Shrestha Y, Oliver R, Tamayo P, Doench JG, 
            Tirosh I, Piccioni F, Hartenian E, Horn H, Fagbami L, Root DE, Jaffe
            J, Lage K, Boehm JS, Hahn WC
  TITLE     Genetic and Proteomic Interrogation of Lower Confidence Candidate 
            Genes Reveals Signaling Networks in beta-Catenin-Active Cancers.
  JOURNAL   Cell Syst. 2016 Sep 28;3(3):302-316.e4. doi: 
            10.1016/j.cels.2016.09.001.
  PUBMED    27684187
REFERENCE   2  (bases 1 to 10704)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2016.09"; journalName: "Cell Syst"; date: 
            "2016-09-28- 28"; volume: "3"; issue: "3"; pages: "302-316.e4"
COMMENT     Sequence Label: JUP_pLX307
FEATURES             Location/Qualifiers
     source          1..10704
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        98..1276
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          329..1267
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1224..1244
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1296..1320
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3556..3580)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3588..3629
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3739..4327
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3792..3812)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4210..4221)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4399..4632
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4710..4831
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4747..4766)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4801..4820
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4871..5006
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5026..5045)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5027..5045)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5055..5072)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5055..5071)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5064..5086)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5213..5668
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5300..5319)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5510..5531
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5694..5798
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5799..5867,5868..6659)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6017..6036)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6830..7418
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7319..7338
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7572..7589
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7706..7727
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7742..7747,7748..7765,7766..7772)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7780..7796
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7785..7807
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7804..7820
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7804..7820
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7839..7859
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7841..7859
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7887..8113
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8114..8294
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8341..8466
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8959..9192
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9377..9421
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9652..9862
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9674..9870
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9721..9856
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9902..10504
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9902..9921)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10398..10418
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10547..10664
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctcgaggc ctgcaggtgc aaagatggat aaagttttaa acagagagga atctttgcag
       61 ctaatggacc ttctaggtct tgaaaggagt gggaattggc tccggtgccc gtcagtgggc
      121 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
      181 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
      241 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
      301 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
      361 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt
      421 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta
      481 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt
      541 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta
      601 aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg
      661 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc
      721 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg
      781 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc
      841 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc
      901 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc
      961 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga
     1021 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac
     1081 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt
     1141 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt
     1201 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc
     1261 atttcaggtg tcgtgaggct agcatcgatt gatcaacaag tttgtacaaa aaagttggca
     1321 tggaggtgat gaacctgatg gagcagccta tcaaggtgac tgagtggcag cagacataca
     1381 cctacgactc gggtatccac tcgggcgcca acacctgcgt gccctccgtc agcagcaagg
     1441 gcatcatgga ggaggatgag gcctgcgggc gccagtacac gctcaagaaa accaccactt
     1501 acacccaggg ggtgcccccc agccaaggtg acctggagta ccagatgtcc acaacagcca
     1561 gggccaaacg ggtgcgggag gccatgtgcc ctggtgtgtc aggcgaggac agctcgcttc
     1621 tgctggccac ccaggtggag gggcaggcca ccaacctgca gcgactggcc gagccgtccc
     1681 agctgctcaa gtcggccatt gtgcatctca tcaactacca ggatgatgcc gagctggcca
     1741 ctcgcgccct gcccgagctc accaaactgc tcaacgacga ggacccggtg gtggtgacca
     1801 aggcggccat gattgtgaac cagctgtcga agaaggaggc gtcgcggcgg gccctgatgg
     1861 gctcgcccca gctggtggcc gctgtcgtgc gtaccatgca gaataccagc gacctggaca
     1921 cagcccgctg caccaccagc atcctgcaca acctctccca ccaccgggag gggctgctcg
     1981 ccatcttcaa gtcgggtggc atccctgctc tggtccgcat gctcagctcc cctgtggagt
     2041 cggtcctgtt ctatgccatc accacgctgc acaacctgct cctgtaccag gagggcgcca
     2101 agatggccgt gcgcctggcc gacgggctgc aaaagatggt gcccctgctc aacaagaaca
     2161 accccaagtt cctggccatc accaccgact gcctgcagct cctggcctac ggcaaccagg
     2221 agagcaagct gatcatcctg gccaatggtg ggccccaggc cctcgtgcag atcatgcgta
     2281 actacagtta tgaaaagctg ctctggacca ccagtcgtgt gctcaaggtg ctatccgtgt
     2341 gtcccagcaa taagcctgcc attgtggagg ctggtgggat gcaggccctg ggcaagcacc
     2401 tgaccagcaa cagcccccgc ctggtgcaga actgcctgtg gaccctgcgc aacctctcag
     2461 atgtggccac caagcaggag ggcctggaga gtgtgctgaa gattctggtg aatcagctga
     2521 gtgtggatga cgtcaacgtc ctcacctgtg ccacgggcac actctccaac ctgacatgca
     2581 acaacagcaa gaacaagacg ctggtgacac agaacagcgg tgtggaggct ctcatccatg
     2641 ccatcctgcg tgctggtgac aaggacgaca tcacggagcc tgccgtctgc gctctgcgcc
     2701 acctcactag ccgccaccct gaggccgaga tggcccagaa ctctgtgcgt ctcaactatg
     2761 gcatcccagc catcgtgaag ctgctcaacc agcccaacca gtggccactg gtcaaggcaa
     2821 ccatcggctt gatcaggaat ctggccctgt gcccagccaa ccatgccccg ctgcaggagg
     2881 cagcggtcat cccccgcctc gtccaactgc tggtgaaggc ccaccaggat gcccagcgcc
     2941 acgtagctgc aggcacacag cagccctaca cggatggtgt gaggatggag gagattgtgg
     3001 agggctgcac cggagcactg cacatcctcg cccgggaccc catgaaccgc atggagatct
     3061 tccggctcaa caccattccc ctgtttgtgc agctcctgta ctcgtcggtg gagaacatcc
     3121 agcgcgtggc tgccggggtg ctgtgtgagc tggcccagga caaggaggcg gccgacgcca
     3181 ttgatgcaga gggggcctcg gccccactca tggagttgct gcactcccgc aacgagggca
     3241 ctgccaccta cgctgctgcc gtcctgttcc gcatctccga ggacaagaac ccagactacc
     3301 ggaagcgcgt gtccgtggag ctcaccaact ccctcttcaa gcatgacccg gctgcctggg
     3361 aggctgccca gagcatgatt cccatcaatg agccctatgg agatgacttg gatgccacct
     3421 accgccccat gtactccagc gatgtgcccc ttgacccgct ggagatgcac atggacatgg
     3481 atggagacta ccccatcgac acctacagcg acggcctcag gcccccgtac cccactgcaa
     3541 accacatgct ggcctaccca actttcttgt acaaagtggt tgatatcggt aagcctatcc
     3601 ctaaccctct cctcggtctc gattctacgt agtaatgaac tagtccgtaa cttgaaagta
     3661 tttgatttct tggctttata tatcttgtgg aaaggacgaa tgcgccaact cttattagat
     3721 aatacgcgtt aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     3781 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     3841 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     3901 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     3961 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     4021 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     4081 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     4141 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     4201 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     4261 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     4321 tccccgcgtc gactttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     4381 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt
     4441 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     4501 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     4561 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     4621 aaatctctag cagtacgtat agtagttcat gtcatcttat tattcagtat ttataacttg
     4681 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca
     4741 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     4801 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct
     4861 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     4921 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     4981 gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg
     5041 tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     5101 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     5161 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc
     5221 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     5281 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     5341 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     5401 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     5461 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     5521 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     5581 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     5641 tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa
     5701 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     5761 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     5821 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     5881 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     5941 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     6001 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     6061 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     6121 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     6181 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     6241 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     6301 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     6361 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     6421 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     6481 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     6541 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     6601 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     6661 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     6721 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     6781 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     6841 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     6901 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     6961 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     7021 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     7081 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     7141 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     7201 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     7261 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     7321 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     7381 ttttgtgatg ctcgtcaggg aggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     7441 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     7501 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     7561 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     7621 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     7681 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     7741 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     7801 acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg
     7861 aacaaaagct ggagctgcaa gcttaatgta gtcttatgca atactcttgt agtcttgcaa
     7921 catggtaacg atgagttagc aacatgcctt acaaggagag aaaaagcacc gtgcatgccg
     7981 attggtggaa gtaaggtggt acgatcgtgc cttattagga aggcaacaga cgggtctgac
     8041 atggattgga cgaaccactg aattgccgca ttgcagagat attgtattta agtgcctagc
     8101 tcgatacata aacgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     8161 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     8221 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     8281 gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag
     8341 ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga
     8401 ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag
     8461 agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca
     8521 gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga
     8581 ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag
     8641 ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca
     8701 accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag
     8761 atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag
     8821 acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
     8881 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
     8941 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
     9001 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
     9061 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
     9121 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
     9181 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
     9241 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
     9301 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
     9361 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
     9421 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
     9481 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
     9541 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccatgca
     9601 ttgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     9661 agtatgcaaa gcatgcgtct caattagtca gcaaccatag tcccgcccct aactccgccc
     9721 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     9781 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     9841 ggcttttttg gaggcctagg cttttgcaaa aagctttcta gaggtaccac cggagcttac
     9901 catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt
     9961 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga
    10021 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
    10081 catcggcaag gtgtgggtcg cggacgacgg cgccgccgtg gcggtctgga ccacgccgga
    10141 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
    10201 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga
    10261 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg
    10321 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
    10381 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
    10441 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgccag
    10501 ttaacgaatt ctagatcttg agacaaatgg cagtattcat ccacaatttt aaaagaaaag
    10561 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
    10621 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
    10681 acagcagaga tccactttgg cgcc
//
