LOCUS       sequence-191217-       11466 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector encoding a doxycycline-inducible Flag-tagged 
            constitutively active MKK6..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11466)
  AUTHORS   Adam AP, Lowery AM, Martino N, Alsaffar H, Vincent PA
  TITLE     Src Family Kinases Modulate the Loss of Endothelial Barrier Function
            in Response to TNF-alpha: Crosstalk with p38 Signaling.
  JOURNAL   PLoS One. 2016 Sep 7;11(9):e0161975. doi: 
            10.1371/journal.pone.0161975. eCollection 2016.
  PUBMED    27603666
REFERENCE   2  (bases 1 to 11466)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-09-7"; volume: "11(9):e0161975. doi"; pages: " 
            10.1371/journal.pone.0161975. eCollection 2016"
COMMENT     Sequence Label: pINDUCER22-MKK6E-Flag
FEATURES             Location/Qualifiers
     source          1..11466
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2230..2248,2249..2271,2272..2290,2291..2313,2314..2332,
                     2333..2355,2356..2374,2375..2397,2398..2416,2417..2439,
                     2440..2458,2459..2481,2482..2500)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2533..2571
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2568..2592
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2701..2725
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2831..2854
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    complement(3868..3892)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3945..3971
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        4057..5268
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5105..5124
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(5327..5944,5945..5950,5951..5986,5987..5989,5990..6025,
                     6026..6028,6029..6064,6065..6073)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    6126..6699
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6293..6310)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6520..6539
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6715..7413
                     /codon_start=1
                     /product="improved green fluorescent protein from 
                     Pontellina plumata (Evdokimov et al., 2006)"
                     /label=TurboGFP
                     /note="mammalian codon-optimized"
                     /translation="MESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKM
                     KSTKGALTFSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVL
                     HVSFSYRYEAGRVIGDFKVMGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNDLDGSFTR
                     TFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFAFRRVEEDHSNTELGIVEYQHAF
                     KTPDADAGEE"
     primer_bind     7549..7568
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    7856..8444
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7909..7929)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8327..8338)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8519..8752
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8871..8889)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8957..9061
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9062..9130,9131..9922)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9280..9299)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10093..10681
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10582..10601
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10835..10852
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11092..11113
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11128..11133,11134..11151,11152..11158)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11166..11182
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11171..11193
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11190..11206
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11190..11206
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11256..11275
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactaggatc
     2221 ctttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca
     2281 gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa
     2341 agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc
     2401 ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa
     2461 agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagctcgg
     2521 tacccgggtc gaggtaggcg tgtacggtgg gaggcctata taagcagagc tcgtttagtg
     2581 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg
     2641 gaccgatcca gcctccgcgg ccccgaacta gtccagtgtg gtggaattct gcagatatca
     2701 acaagtttgt acaaaaaagc aggcttcgaa ggagatagaa ccaattctct aaggaaatac
     2761 ttaaccatgg tcgactggat ccggtaccga attcgcggcc gcactcgaga tagcttcggc
     2821 agccaacatg gactataagg acgatgatga caaatctcag tcgaaaggca agaagcgaaa
     2881 ccctggcctt aaaattccaa aagaagcatt tgaacaacct cagaccagtt ccacaccacc
     2941 tagagattta gactccaagg cttgcatttc tattggaaat cagaactttg aggtgaaggc
     3001 agatgacctg gagcctataa tggaactggg acgaggtgcg tacggggtgg tggagaagat
     3061 gcggcacgtg cccagcgggc agatcatggc agtgaagcgg atccgagcca cagtaaatag
     3121 ccaggaacag aaacggctac tgatggattt ggatatttcc atgaggacgg tggactgtcc
     3181 attcactgtc accttttatg gcgcactgtt tcgggagggt gatgtgtgga tctgcatgga
     3241 gctcatggat acatcactag ataaattcta caaacaagtt attgataaag gccagacaat
     3301 tccagaggac atcttaggga aaatagcagt ttctattgta aaagcattag aacatttaca
     3361 tagtaagctg tctgtcattc acagagacgt caagccttct aatgtactca tcaatgctct
     3421 cggtcaagtg aagatgtgcg attttggaat cagtggctac ttggtggacg aggttgctaa
     3481 agaaattgat gcaggttgca aaccatacat ggcccctgaa agaataaacc cagagctcaa
     3541 ccagaaggga tacagtgtga agtctgacat ttggagtctg ggcatcacga tgattgagtt
     3601 ggccatcctt cgatttccct atgattcatg gggaactcca tttcagcagc tcaaacaggt
     3661 ggtagaggag ccatcgccac aactcccagc agacaagttc tctgcagagt ttgttgactt
     3721 tacctcacag tgcttaaaga agaattccaa agaacggcct acatacccag agctaatgca
     3781 acatccattt ttcaccctac atgaatccaa aggaacagat gtggcatctt ttgtaaaact
     3841 gattcttgga gactaatcta gatctagacc cagctttctt gtacaaagtg gttgatatcc
     3901 agcacagtgg cggccgctcg agtctagagg gcccgcggtt cgaataccca tacgacgtcc
     3961 cagactacgc ttagtaatga ttaattaaac tagaaattct accgggtagg ggaggcgctt
     4021 ttcccaaggc agtctggagt aacccaccca agatctggcc tccgcgccgg gttttggcgc
     4081 ctcccgcggg cgcccccctc ctcacggcga gcgctgccac gtcagacgaa gggcgcagcg
     4141 agcgtcctga tccttccgcc cggacgctca ggacagcggc ccgctgctca taagactcgg
     4201 ccttagaacc ccagtatcag cagaaggaca ttttaggacg ggacttgggt gactctaggg
     4261 cactggtttt ctttccagag agcggaacag gcgaggaaaa gtagtccctt ctcggcgatt
     4321 ctgcggaggg atctccgtgg ggcggtgaac gccgatgatt atataaggac gcgccgggtg
     4381 tggcacagct agttccgtcg cagccgggat ttgggtcgcg gttcttgttt gtggatcgct
     4441 gtgatcgtca cttggtgagt agcgggctgc tgggctggcc ggggctttcg tggccgccgg
     4501 gccgctcggt gggacggaag cgtgtggaga gaccgccaag ggctgtagtc tgggtccgcg
     4561 agcaaggttg ccctgaactg ggggttgggg ggagcgcagc aaaatggcgg ctgttcccga
     4621 gtcttgaatg gaagacgctt gtgaggcggg ctgtgaggtc gttgaaacaa ggtggggggc
     4681 atggtgggcg gcaagaaccc aaggtcttga ggccttcgct aatgcgggaa agctcttatt
     4741 cgggtgagat gggctggggc accatctggg gaccctgacg tgaagtttgt cactgactgg
     4801 agaactcggt ttgtcgtctg ttgcgggggc ggcagttatg gcggtgccgt tgggcagtgc
     4861 acccgtacct ttgggagcgc gcgccctcgt cgtgtcgtga cgtcacccgt tctgttggct
     4921 tataatgcag ggtggggcca cctgccggta ggtgtgcggt aggcttttct ccgtcgcagg
     4981 acgcagggtt cgggcctagg gtaggctctc ctgaatcgac aggcgccgga cctctggtga
     5041 ggggagggat aagtgaggcg tcagtttctt tggtcggttt tatgtaccta tcttcttaag
     5101 tagctgaagc tccggttttg aactatgcgc tcggggttgg cgagtgtgtt ttgtgaagtt
     5161 ttttaggcac cttttgaaat gtaatcattt gggtcaatat gtaattttca gtgttagact
     5221 agtaaattgt ccgctaaatt ctggccgttt ttggcttttt tgttagacga agcttggtac
     5281 cgagctcgga tctccacccc gtaccggtcc tgcagtcgaa ttcaccatgt ctagactgga
     5341 caagagcaaa gtcataaacg gagctctgga attactcaat ggtgtcggta tcgaaggcct
     5401 gacgacaagg aaactcgctc aaaagctggg agttgagcag cctaccctgt actggcacgt
     5461 gaagaacaag cgggccctgc tcgatgccct gccaatcgag atgctggaca ggcatcatac
     5521 ccacttctgc cccctggaag gcgagtcatg gcaagacttt ctgcggaaca acgccaagtc
     5581 ataccgctgt gctctcctct cacatcgcga cggggctaaa gtgcatctcg gcacccgccc
     5641 aacagagaaa cagtacgaaa ccctggaaaa tcagctcgcg ttcctgtgtc agcaaggctt
     5701 ctccctggag aacgcactgt acgctctgtc cgccgtgggc cactttacac tgggctgcgt
     5761 attggaggaa caggagcatc aagtagcaaa agaggaaaga gagacaccta ccaccgattc
     5821 tatgccccca cttctgagac aagcaattga gctgttcgac cggcagggag ccgaacctgc
     5881 cttccttttc ggcctggaac taatcatatg tggcctggag aaacagctaa agtgcgaaag
     5941 cggcgggccg accgacgccc ttgacgattt tgacttagac atgctcccag ccgatgccct
     6001 tgacgacttt gaccttgata tgctgcctgc tgacgctctt gacgattttg accttgacat
     6061 gctccccggg taactaagta aggatccgcg gccgcactag aggaattccg cccctctccc
     6121 ccccccccct ctccctcccc cccccctaac gttactggcc gaagccgctt ggaataaggc
     6181 cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg
     6241 gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc
     6301 aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga
     6361 agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg
     6421 tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag
     6481 tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc
     6541 aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct
     6601 cggtgcacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac
     6661 cacggggacg tggttttcct ttgaaaaaca cgatgataag cttaccggtc caccatggag
     6721 agcgacgaga gcggcctgcc cgccatggag atcgagtgcc gcatcaccgg caccctgaac
     6781 ggcgtggagt tcgagctggt gggcggcgga gagggcaccc ccgagcaggg ccgcatgacc
     6841 aacaagatga agagcaccaa aggcgccctg accttcagcc cctacctgct gagccacgtg
     6901 atgggctacg gcttctacca cttcggcacc taccccagcg gctacgagaa ccccttcctg
     6961 cacgccatca acaacggcgg ctacaccaac acccgcatcg agaagtacga ggacggcggc
     7021 gtgctgcacg tgagcttcag ctaccgctac gaggccggcc gcgtgatcgg cgacttcaag
     7081 gtgatgggca ccggcttccc cgaggacagc gtgatcttca ccgacaagat catccgcagc
     7141 aacgccaccg tggagcacct gcaccccatg ggcgataacg atctggatgg cagcttcacc
     7201 cgcaccttca gcctgcgcga cggcggctac tacagctccg tggtggacag ccacatgcac
     7261 ttcaagagcg ccatccaccc cagcatcctg cagaacgggg gccccatgtt cgccttccgc
     7321 cgcgtggagg aggatcacag caacaccgag ctgggcatcg tggagtacca gcacgccttc
     7381 aagaccccgg atgcagatgc cggtgaagaa taagcatgcc cgacggcgag gatctcgtcg
     7441 tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat
     7501 tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc
     7561 gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta
     7621 tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgat
     7681 gtacaagtaa agcggccgcg actctagatc ataatcagcc ataccacatt tgtagaggtt
     7741 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
     7801 attgttgttg tttagtccct cccaattcga tatcaagctt atcgcgatag atcctaatca
     7861 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     7921 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     7981 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     8041 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     8101 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     8161 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     8221 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg
     8281 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     8341 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     8401 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctgaga tcctttaaga
     8461 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     8521 gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc
     8581 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     8641 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     8701 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt
     8761 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag
     8821 aggcccgggt taattaagga aagggctaga tcattcttga agacgaaagg gcctcgtgat
     8881 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
     8941 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     9001 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     9061 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     9121 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     9181 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     9241 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     9301 ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     9361 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     9421 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     9481 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     9541 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     9601 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     9661 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     9721 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     9781 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     9841 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     9901 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     9961 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
    10021 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
    10081 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
    10141 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
    10201 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
    10261 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
    10321 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
    10381 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
    10441 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
    10501 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
    10561 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
    10621 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
    10681 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
    10741 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
    10801 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
    10861 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagcaa
    10921 gctcatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
    10981 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccc
    11041 gtggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
    11101 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
    11161 tggaattgtg agcggataac aatttcacac aggaaacagc tatgacatga ttacgaattt
    11221 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
    11281 atcttatcat gtctggatca actggataac tcaagctaac caaaatcatc ccaaacttcc
    11341 caccccatac cctattacca ctgccaatta cctgtggttt catttactct aaacctgtga
    11401 ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt taaatatgta ctacaaactt
    11461 agtagt
//
