LOCUS       sequence-191219-         744 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses SaCas9 and a gRNA targeting mouse YAP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Wang L, Luo JY, Li B, Tian XY, Chen LJ, Huang Y, Liu J, Deng D, Lau 
            CW, Wan S, Ai D, Mak KK, Tong KK, Kwan KM, Wang N, Chiu JJ, Zhu Y, 
            Huang Y
  TITLE     Integrin-YAP/TAZ-JNK cascade mediates atheroprotective effect of 
            unidirectional shear flow.
  JOURNAL   Nature. 2016 Dec 7. doi: 10.1038/nature20602.
  PUBMED    27926730
REFERENCE   2  (bases 1 to 744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2016 Dec 7. doi: 10.1038/nature20602."
COMMENT     Sequence Label: Icam 2 SaCas9 YAP sgRNA3
FEATURES             Location/Qualifiers
     source          1..744
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(264..284)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      287..296
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ctgtggtcgg tcagcaggtt gtagtcgaac agcagcttct tcactctctg gattctatgc
       61 cgcctccgcc gcttcagcct tctggcgcct ctcttgctcc gcctgccctc gttgttttcc
      121 acgttggcct ctttgaacag ccgcacgccg gcatcgatca cgtcccgtgt ctcgtagtcg
      181 atgatgccgt agcccacgct ggtgatgccg atgtccaggc ccaggatgta gttccgcttg
      241 gctgctggga ctccgtggat accgaccttc cgcttcttct ttggggccat ggtggcaccg
      301 gtgactggca gtctccacgg gctcgcaggg accagccaag ggctgcctgg agggagatgg
      361 tgggcgcagg tctgagctat ggcccagaat ccctagcctt ctgcaaactg atgactgcat
      421 ttcctctcat tatctgagag atctttggga agccacgtgc accagctcgt tctaggaccc
      481 caggcctcta agaatccagg cagaggaagc tgggaagctg ctgataagca gggcataacc
      541 cagtcctccc tgctgactcc ctgtgcaagg ggcggggcag gcaatgggtg cacgcatgcg
      601 tgtatgagac gtgtgcacag gggagagtca ggcctacaag ccccagatct ggggaacccc
      661 aaatcccatg ggatgcattg ttggagtcat gcctgggaga cctcgagtct agaggccgca
      721 ggaaccccta gtgatggagt tggc
//
