LOCUS       sequence-19124-e         780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid was designed for screening purposes and may contain 
            discrepancies relative to the current canonical GenBank version of 
            the gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, 
            Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW
  TITLE     Identification of poxvirus CD8+ T cell determinants to enable 
            rational design and characterization of smallpox vaccines.
  JOURNAL   J Exp Med. 2005 January 3; 201(1): 95–104.
  PUBMED    15623576
REFERENCE   2  (bases 1 to 780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "2005-?-3"; volume: " 201(1)"; pages: " 95–104"
COMMENT     Sequence Label: DCT.VV.Orf41
FEATURES             Location/Qualifiers
     source          1..780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             585..626
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             636..653
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(676..693)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 ggtaccgagc tcggatccag tacccttcac cgaattccag ctgaccacca tggaaatgga
       61 taagcgtatg aaatctctcg caatgaccgc tttctttggg gagctaagca cattagatat
      121 tatggcattg ataatgtcta tatttaaacg ccatccaaac aataccattt tttcagtgga
      181 taaggatggt cagtttatga ttgatttcga atacgataat tataaggctt ctcaatattt
      241 ggatctgacc ctcactccga tatctggaga tgaatgcaag actcacgcat cgagtatagc
      301 cgaacaattg gcgtgtgtgg atattattaa agaggatatt agcgaatata tcaaaactac
      361 tccccgtctt aaacgattta taaaaaaata ccgcaataga tcagatactc gcatcagtcg
      421 agatacagaa aagcttaaaa tagctctagc taaaggcata gattacgaat atataaaaga
      481 cgcttgttaa gcaattcccg gggatccgaa taaagggtca agacaattct gcagatatcc
      541 agcacagtgg cggccgctcg agtctagagg gcccgcggtt cgaaggtaag cctatcccta
      601 accctctcct cggtctcgat tctacgcgta ccggtcatca tcaccatcac cattgagttt
      661 aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccaatctgt tgtttgcccc
      721 tcccccgtgc cttcctggac cctggaaagt gccactccca ctgtcctttc ctaataaatg
//
