LOCUS       sequence-191259-        3243 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor Vector containing HOXC5 transcription factor, part of the 
            Human TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3243)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 3243)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR221-HOXC5
FEATURES             Location/Qualifiers
     source          1..3243
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(668..692)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(698..797)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(814..833)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(815..833)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(838..854)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(838..854)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(956..975)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             967..1776
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1034..1053)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      1923..2511
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2412..2431
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2665..2682
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2841..2868)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2960..3046)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3095..3117
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3109..3126
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3110..3126
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3142..3241
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 atgagctcct acgtagccaa ttcattctat aagcagagcc ccaatatccc tgcctataac
       61 atgcaaactt gtgggaacta tggatcggcc tcagaggtgc aggcatccag gtactgctac
      121 ggcggattgg acttaagcat cactttccca ccgcctgcgc cttccaactc tctacacggg
      181 gtagacatgg ctgcaaaccc cagagctcat cctgacagac cagcatgtag tgccgcagca
      241 gctccgggac atgctccggg tagagatgaa gcggctcctt tgaacccagg gatgtacagt
      301 caaaaggcgg ctcgaccagc actggaggag cgagctaaga gcagtgggga gattaaagag
      361 gagcaggcac agacagggca gcctgccgga ttaagtcagc caccagcccc gccacagatt
      421 tacccgtgga tgaccaaact gcacatgagc cacgagacgg acggcaagcg atcccgaacc
      481 agttacacgc gctaccagac tctggaactc gagaaagaat tccactttaa ccgctacctc
      541 actcgccgca ggcgcataga gatcgccaac aacttgtgtc tcaatgagag acagatcaag
      601 atctggttcc agaaccgcag gatgaagtgg aagaaagatt ccaaaatgaa aagcaaagag
      661 gctctttacc caactttctt gtacaaagtt gtcccctacc cagctttctt gtacaaagtt
      721 ggcattataa gaaagcattg cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa
      781 aataaaatca ttatttgcca tccagctgat atcccctata gtgagtcgta ttacatggtc
      841 atagctgttt cctggcagct ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa
      901 gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag taatacaagg
      961 ggtgttatga gccatattca acgggaaacg tcgaggccgc gattaaattc caacatggat
     1021 gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc
     1081 tatcgcttgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc
     1141 gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcct
     1201 cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg
     1261 atccccggaa aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt
     1321 gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct
     1381 tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg
     1441 gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa
     1501 gaaatgcata aacttttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca
     1561 cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc
     1621 ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct
     1681 ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa
     1741 ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa ttggttgtaa
     1801 cactggcaga gcattacgct gacttgacgg gacggcgcaa gctcatgacc aaaatccctt
     1861 aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     1921 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     1981 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     2041 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     2101 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     2161 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     2221 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     2281 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     2341 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     2401 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     2461 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     2521 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     2581 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     2641 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     2701 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     2761 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atacgcgtac cgctagccag
     2821 gaagagtttg tagaaacgca aaaaggccat ccgtcaggat ggccttctgc ttagtttgat
     2881 gcctggcagt ttatggcggg cgtcctgccc gccaccctcc gggccgttgc ttcacaacgt
     2941 tcaaatccgc tcccggcgga tttgtcctac tcaggagagc gttcaccgac aaacaacaga
     3001 taaaacgaaa ggcccagtct tccgactgag cctttcgttt tatttgatgc ctggcagttc
     3061 cctactctcg cgttaacgct agcatggatg ttttcccagt cacgacgttg taaaacgacg
     3121 gccagtctta agctcgggcc ccaaataatg attttatttt gactgatagt gacctgttcg
     3181 ttgcaacaaa ttgatgagca atgctttttt ataatgccaa ctttgtacaa aaaagcaggc
     3241 tta
//
