LOCUS       sequence-19126-e         697 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid was designed for screening purposes and may contain 
            discrepancies relative to the current canonical GenBank version of 
            the gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, 
            Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW
  TITLE     Identification of poxvirus CD8+ T cell determinants to enable 
            rational design and characterization of smallpox vaccines.
  JOURNAL   J Exp Med. 2005 January 3; 201(1): 95–104.
  PUBMED    15623576
REFERENCE   2  (bases 1 to 697)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "2005-?-3"; volume: " 201(1)"; pages: " 95–104"
COMMENT     Sequence Label: DCT.VV.Orf43
FEATURES             Location/Qualifiers
     source          1..697
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             366..383
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(406..423)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    412..523
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 aaatgcatta aataattcca atagagcgat ttttgttcct ataggacctt ccaactgtgg
       61 atactctgta ttgttaatag atatattaat acttttgtcg ggtaacagag gttctacgtc
      121 ttttaaaaat aaaagtttga taacatctgg cctgttcata aataaaaact tggcgattct
      181 atatatactc ttattatcaa atctagccat tgtcttataa gccattcccg gggattccaa
      241 taaagggtca agaacattct gccaaatatc cgcacagtgg cggccgctcg agtctagagg
      301 ggccgcgggt tcgaagtaag cctatcccta accctctcct cggtctcgat tctacgcgta
      361 ccggtcatca tcaccatcac cattgagttt aaacccgctg atcagcctcg actgtgcctt
      421 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
      481 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
      541 tcttcatctg gggggggggg ggggggtggg gcaggacgca aggggggaag aattgggaag
      601 acaatagcag gcatgctggg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc
      661 agctggggct ctagggggta tcccacgcgc cctgtag
//
