LOCUS       sequence-191281-        3276 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor Vector containing BSX transcription factor, part of the Human 
            TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3276)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 3276)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR221-BSX
FEATURES             Location/Qualifiers
     source          1..3276
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(701..725)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(731..830)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(847..866)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(848..866)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(871..887)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(871..887)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(989..1008)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1000..1809
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1067..1086)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      1956..2544
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2445..2464
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2698..2715
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2874..2901)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2993..3079)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3128..3150
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3142..3159
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3143..3159
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3175..3274
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 atgaatctca acttcacctc tcctctacac ccggcgtctt ctcagaggcc cacatccttc
       61 ttcatcgagg acatcctgct gcacaagccc aagccgctga gagaggtggc cccagaccat
      121 ttcgccagct ctctggcctc tcgtgtgcct cttctagact atggctatcc cctcatgccc
      181 acacccaccc tcttggctcc tcacgcccat caccctctgc ataagggtga ccaccatcat
      241 ccttatttcc ttaccacctc ggggatgcca gtcccagcgc tattcccgca ccctcaacac
      301 gcggagctac ctgggaaaca ttgtcgtcgc cgcaaagcac gcacggtttt ctctgactcg
      361 cagctctcgg gcttggagaa gaggttcgag attcagcgct acctgtccac gccagaacga
      421 gtggagcttg ccacggccct cagcctatcc gagacgcagg tcaaaacgtg gttccagaac
      481 cggcggatga agcataaaaa gcaactgcgg aaaagccaag acgaacccaa agcaccagat
      541 gggccagaaa gcccagaagg aagtccccgc ggttcagaag ctgctaccgc tgctgaagct
      601 cggcttagcc tacctgccgg tcccttcgta ctgactgagc cagaggacga ggtggatatt
      661 ggagacgagg gtgagctggg ctcaggtccg cacgttctct gcccaacttt cttgtacaaa
      721 gttgtcccct acccagcttt cttgtacaaa gttggcatta taagaaagca ttgcttatca
      781 atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg ccatccagct
      841 gatatcccct atagtgagtc gtattacatg gtcatagctg tttcctggca gctctggccc
      901 gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc atgaacaata
      961 aaactgtctg cttacataaa cagtaataca aggggtgtta tgagccatat tcaacgggaa
     1021 acgtcgaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct
     1081 cgcgataatg tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa gcccgatgcg
     1141 ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg
     1201 gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt
     1261 actcctgatg atgcatggtt actcaccact gcgatccccg gaaaaacagc attccaggta
     1321 ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc
     1381 cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc
     1441 gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag
     1501 cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataaactttt gccattctca
     1561 ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg
     1621 aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt
     1681 gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa
     1741 aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag
     1801 tttttctaat cagaattggt taattggttg taacactggc agagcattac gctgacttga
     1861 cgggacggcg caagctcatg accaaaatcc cttaacgtga gttacgcgtc gttccactga
     1921 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     1981 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     2041 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     2101 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     2161 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     2221 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     2281 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     2341 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     2401 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     2461 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     2521 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     2581 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     2641 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     2701 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     2761 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     2821 cgcaacgcaa ttaatacgcg taccgctagc caggaagagt ttgtagaaac gcaaaaaggc
     2881 catccgtcag gatggccttc tgcttagttt gatgcctggc agtttatggc gggcgtcctg
     2941 cccgccaccc tccgggccgt tgcttcacaa cgttcaaatc cgctcccggc ggatttgtcc
     3001 tactcaggag agcgttcacc gacaaacaac agataaaacg aaaggcccag tcttccgact
     3061 gagcctttcg ttttatttga tgcctggcag ttccctactc tcgcgttaac gctagcatgg
     3121 atgttttccc agtcacgacg ttgtaaaacg acggccagtc ttaagctcgg gccccaaata
     3181 atgattttat tttgactgat agtgacctgt tcgttgcaac aaattgatga gcaatgcttt
     3241 tttataatgc caactttgta caaaaaagca ggctta
//
