LOCUS       sequence-19131-e         694 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid was designed for screening purposes and may contain 
            discrepancies relative to the current canonical GenBank version of 
            the gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 694)
  AUTHORS   Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, 
            Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW
  TITLE     Identification of poxvirus CD8+ T cell determinants to enable 
            rational design and characterization of smallpox vaccines.
  JOURNAL   J Exp Med. 2005 January 3; 201(1): 95–104.
  PUBMED    15623576
REFERENCE   2  (bases 1 to 694)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "2005-?-3"; volume: " 201(1)"; pages: " 95–104"
COMMENT     Sequence Label: DCT.VV.Orf48
FEATURES             Location/Qualifiers
     source          1..694
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             587..628
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             638..655
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 accaccatgg cgagctttat taccaatgga taccttccag ttacattgga accacacgag
       61 ctgacgttag acataaaaac taatattagg aatgccgtat ataagacgta tctccataga
      121 gaaattagtg gtaaaatggc caagaaaata gaaattcgtg aagacgtgga attacctctc
      181 ggcgaaatag ttaataattc tgtagttata aacgttccgt gtgtaataac ctacgcgtat
      241 tatcacgttg gggatatagt cagaggaaca ttaaacatcg aagatgaatc aaatgtaact
      301 attcaatgtg gagatttaat ctgtaaacta agtagagatt cgggtactgt atcatttagc
      361 gattcaaagt actgcttttt tcgaaatggt aatgcgtatg acaatggcag cgaagtcact
      421 gccgttctaa tggaggctca acaaggtatc gaatctagtt ttgtttttct cgcgaatatc
      481 gtcgactcat aagcaattcc cggggatccg aataaagggt caagacaatt ctgcagatat
      541 ccagcacagt ggcggccgct cgagtctaga gggcccgcgg ttcgaaggta agcctatccc
      601 taaccctctc ctcggtctcg attctacgcg taccggtcat catcaccatc accattgagt
      661 ttaaacccgc tgatcagcct cgactgtgcc ttct
//
