LOCUS       sequence-191319-        3504 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor Vector containing FOXE3 transcription factor, part of the 
            Human TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3504)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 3504)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR221-FOXE3
FEATURES             Location/Qualifiers
     source          1..3504
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(959..1058)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1075..1094)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1076..1094)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1099..1115)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1099..1115)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1217..1236)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1228..2037
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1295..1314)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2184..2772
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2673..2692
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2926..2943
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3102..3129)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3221..3307)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3356..3378
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3370..3387
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3371..3387
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3403..3502
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 atggccggca gaagcgacat ggatccaccc gctgcctttt ctggcttccc tgcactgcca
       61 gccgttgccc cttctggacc tcctccatct cctcttgctg gagctgagcc tggaagagag
      121 cctgaggaag cagcagctgg cagaggagag gctgctccta cccctgctcc tggaccaggc
      181 agaagaagaa ggaggcctct tcagagaggc aagccacctt acagctacat cgccctgatt
      241 gccatggccc tggcccacgc ccctgggaga agactgacac tggccgccat ctacagattc
      301 atcaccgaga gattcgcctt ctacagagac tcccccagaa agtggcagaa cagcatcaga
      361 cacaacctga ccctgaacga ctgctttgtg aaggtgccca gagagccagg caatcccggc
      421 aagggcaact actggacact ggaccctgcc gccgccgaca tgttcgacaa cggcagcttc
      481 ctgagaagga gaaagagatt caaaagagcc gagctgcctg cccatgcagc cgccgctcca
      541 ggccctcctc ttccttttcc ttacgctcct tatgctcctg cacctggacc tgccctgtta
      601 gttcctcctc cctctgcagg acctggccct agccctcccg ctagactgtt tagcgtggac
      661 agcctggtga acctgcagcc tgagttagct ggactgggag ctcctgagcc tccttgctgt
      721 gcagcaccag atgctgctgc cgcagccttt cctccttgtg ctgcagctgc ctctccccct
      781 ctgtatagcc aggtgcctga cagactggtg cttcccgcta caagaccagg acccggacct
      841 ctgcctgctg aacctctgct tgccttagcc ggaccagccg ctgctttggg acctttgtct
      901 ccaggcgaag cttacctgag acagccaggc ttcgccagcg gcctggaaag atacctgtac
      961 ccagctttct tgtacaaagt tggcattata agaaagcatt gcttatcaat ttgttgcaac
     1021 gaacaggtca ctatcagtca aaataaaatc attatttgcc atccagctga tatcccctat
     1081 agtgagtcgt attacatggt catagctgtt tcctggcagc tctggcccgt gtctcaaaat
     1141 ctctgatgtt acattgcaca agataaaaat atatcatcat gaacaataaa actgtctgct
     1201 tacataaaca gtaatacaag gggtgttatg agccatattc aacgggaaac gtcgaggccg
     1261 cgattaaatt ccaacatgga tgctgattta tatgggtata aatgggctcg cgataatgtc
     1321 gggcaatcag gtgcgacaat ctatcgcttg tatgggaagc ccgatgcgcc agagttgttt
     1381 ctgaaacatg gcaaaggtag cgttgccaat gatgttacag atgagatggt cagactaaac
     1441 tggctgacgg aatttatgcc tcttccgacc atcaagcatt ttatccgtac tcctgatgat
     1501 gcatggttac tcaccactgc gatccccgga aaaacagcat tccaggtatt agaagaatat
     1561 cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg
     1621 attcctgttt gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa
     1681 tcacgaatga ataacggttt ggttgatgcg agtgattttg atgacgagcg taatggctgg
     1741 cctgttgaac aagtctggaa agaaatgcat aaacttttgc cattctcacc ggattcagtc
     1801 gtcactcatg gtgatttctc acttgataac cttatttttg acgaggggaa attaataggt
     1861 tgtattgatg ttggacgagt cggaatcgca gaccgatacc aggatcttgc catcctatgg
     1921 aactgcctcg gtgagttttc tccttcatta cagaaacggc tttttcaaaa atatggtatt
     1981 gataatcctg atatgaataa attgcagttt catttgatgc tcgatgagtt tttctaatca
     2041 gaattggtta attggttgta acactggcag agcattacgc tgacttgacg ggacggcgca
     2101 agctcatgac caaaatccct taacgtgagt tacgcgtcgt tccactgagc gtcagacccc
     2161 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     2221 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     2281 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     2341 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     2401 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     2461 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     2521 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     2581 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     2641 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     2701 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     2761 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     2821 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     2881 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     2941 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     3001 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     3061 aatacgcgta ccgctagcca ggaagagttt gtagaaacgc aaaaaggcca tccgtcagga
     3121 tggccttctg cttagtttga tgcctggcag tttatggcgg gcgtcctgcc cgccaccctc
     3181 cgggccgttg cttcacaacg ttcaaatccg ctcccggcgg atttgtccta ctcaggagag
     3241 cgttcaccga caaacaacag ataaaacgaa aggcccagtc ttccgactga gcctttcgtt
     3301 ttatttgatg cctggcagtt ccctactctc gcgttaacgc tagcatggat gttttcccag
     3361 tcacgacgtt gtaaaacgac ggccagtctt aagctcgggc cccaaataat gattttattt
     3421 tgactgatag tgacctgttc gttgcaacaa attgatgagc aatgcttttt tataatgcca
     3481 actttgtaca aaaaagcagg ctta
//
