LOCUS       sequence-191323-        3642 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor Vector containing FOXH1 transcription factor, part of the 
            Human TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3642)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 3642)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR221-FOXH1
FEATURES             Location/Qualifiers
     source          1..3642
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1097..1196)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1213..1232)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1214..1232)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1237..1253)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1237..1253)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1355..1374)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1366..2175
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1433..1452)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2322..2910
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2811..2830
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3064..3081
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3240..3267)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3359..3445)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3494..3516
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3508..3525
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3509..3525
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3541..3640
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 atgggcccct gtagcggcag cagacttggc ccccccgaag ccgaaagccc ttctcagcct
       61 cccaagagaa gaaagaagag atatctgaga catgataagc ctccctacac atacctggct
      121 atgatcgccc tggtgattca ggcagctcca tctagaagac tgaagctggc ccagatcatc
      181 agacaggtgc aggccgtgtt ccccttcttc agagaggatt acgagggctg gaaggacagc
      241 atcaggcaca acctgagctc taacagatgc ttcagaaagg tgcctaagga tcccgccaag
      301 cctcaggcta agggcaattt ttgggccgtg gatgtgtctc tgattcctgc tgaggctctc
      361 agactgcaga ataccgccct gtgtagaaga tggcagaatg gaggcgctag aggcgctttt
      421 gctaaagacc tgggacccta tgtgctgcat ggaagacctt atagaccccc ttcaccacct
      481 ccacctccta gcgaaggctt cagcatcaag tctctgttag gaggatctgg agaaggcgct
      541 ccttggcctg gattagctcc acagtcatct ccagtgcctg ctggaaccgg caatagcgga
      601 gaagaagctg ttcctacacc tcctcttccc tcttctgaga ggccactttg gcctttgtgc
      661 cctcttcctg gacctacaag agtggaggga gaaacagtgc agggaggagc tatcggacct
      721 tctaccctgt ctcctgaacc tagagcttgg cctctgcatc ttttacaggg aacagccgtt
      781 cctggaggaa ggagctctgg aggacacaga gcctctcttt ggggacagct gcctacctct
      841 tatctgccta tctacacacc caatgtggtg atgcctctgg ctcctcctcc tacatcttgc
      901 ccccagtgtc cttctacatc tcctgcttat tggggagtgg ctcctgaaac cagaggacct
      961 cctggactgc tttgcgatct ggatgccctt ttccagggcg tgcctcctaa taagagcatc
     1021 tatgacgtgt gggtgtctca tcctagagat ctggctgctc ccggacctgg ctggcttctg
     1081 tcttggtgta gcctgtaccc agctttcttg tacaaagttg gcattataag aaagcattgc
     1141 ttatcaattt gttgcaacga acaggtcact atcagtcaaa ataaaatcat tatttgccat
     1201 ccagctgata tcccctatag tgagtcgtat tacatggtca tagctgtttc ctggcagctc
     1261 tggcccgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat atcatcatga
     1321 acaataaaac tgtctgctta cataaacagt aatacaaggg gtgttatgag ccatattcaa
     1381 cgggaaacgt cgaggccgcg attaaattcc aacatggatg ctgatttata tgggtataaa
     1441 tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgcttgta tgggaagccc
     1501 gatgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga tgttacagat
     1561 gagatggtca gactaaactg gctgacggaa tttatgcctc ttccgaccat caagcatttt
     1621 atccgtactc ctgatgatgc atggttactc accactgcga tccccggaaa aacagcattc
     1681 caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc
     1741 ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt
     1801 cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag tgattttgat
     1861 gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcataa acttttgcca
     1921 ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct tatttttgac
     1981 gaggggaaat taataggttg tattgatgtt ggacgagtcg gaatcgcaga ccgataccag
     2041 gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca gaaacggctt
     2101 tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca tttgatgctc
     2161 gatgagtttt tctaatcaga attggttaat tggttgtaac actggcagag cattacgctg
     2221 acttgacggg acggcgcaag ctcatgacca aaatccctta acgtgagtta cgcgtcgttc
     2281 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     2341 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     2401 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     2461 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     2521 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     2581 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     2641 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     2701 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     2761 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     2821 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     2881 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     2941 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     3001 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     3061 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     3121 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     3181 agtgagcgca acgcaattaa tacgcgtacc gctagccagg aagagtttgt agaaacgcaa
     3241 aaaggccatc cgtcaggatg gccttctgct tagtttgatg cctggcagtt tatggcgggc
     3301 gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt caaatccgct cccggcggat
     3361 ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt
     3421 ccgactgagc ctttcgtttt atttgatgcc tggcagttcc ctactctcgc gttaacgcta
     3481 gcatggatgt tttcccagtc acgacgttgt aaaacgacgg ccagtcttaa gctcgggccc
     3541 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
     3601 tgctttttta taatgccaac tttgtacaaa aaagcaggct ta
//
