LOCUS       sequence-191340-        1119 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Donor Vector containing FOXE1 transcription factor, part of the 
            Human TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1119)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 1119)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR221-FOXE1
FEATURES             Location/Qualifiers
     source          1..1119
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             62..73
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     RBS             567..575
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 atgaccgccg agagcggacc acctcctcct cagcctgaag tgctggctac agtgaaagaa
       61 gaaagaggag aaacagccgc cggagccggc gttcctggag aggctacagg cagaggcgct
      121 ggcggcagga gaagaaaaag accacttcag agaggcaagc ctccctacag ctacatcgcc
      181 ctgatcgcca tggccatcgc ccacgccccc gagagaagac tgacactggg cggcatctac
      241 aagttcatca ccgagagatt ccccttctac agggacaacc ccaagaagtg gcagaacagc
      301 atcagacaca acctgaccct gaatgactgc ttcctcaaga tcccccggga ggctggccgg
      361 cctggcaagg gcaattactg ggccctggac cccaatgccg aggacatgtt tgagtctggc
      421 agcttcctga gaagaaggaa gagattcaag agaagcgacc tgagcacata ccctgcctac
      481 atgcatgacg cagctgctgc agctgccgct gctgccgcag cagcagccgc tgcggctata
      541 tttccaggag ctgttcctgc agctagacct ccttatcctg gagctgtgta tgccggctac
      601 gctccccctt ctctggctgc tccacctccc gtgtactatc ctgctgccag cccaggacct
      661 tgcagagtgt tcggcttggt gcctgaaaga cctctgagcc ctgaactcgg ccccgctcct
      721 tctgggcctg gaggatcttg tgcttttgcc tcagccggcg ctcctgctac aacaacagga
      781 tatcagcctg ctggctgtac aggagctagg cctgctaacc ctagcgccta tgccgccgct
      841 tacgccggac ctgatggagc atatcctcag ggagctggat ctgctatttt tgcggccgct
      901 ggcagactgg ctggacctgc ttctcctccc gctggaggct ctagcggagg agtggagaca
      961 accgtggatt tctacggaag aacaagccct ggccagtttg gagctctggg cgcctgttat
     1021 aatccaggag gacagctggg cggagcctct gctggagctt accatgccag acacgctgcc
     1081 gcctaccctg gcggaattga cagattcgtg agcgccatg
//
