LOCUS       sequence-191343-        4445 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor Vector containing ZNF860 transcription factor, part of the 
            Human TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4445)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 4445)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR221-ZNF860
FEATURES             Location/Qualifiers
     source          1..4445
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1900..1999)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2016..2035)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2017..2035)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2040..2056)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2040..2056)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2158..2177)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2169..2978
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2236..2255)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3125..3713
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3614..3633
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3867..3884
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4043..4070)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4162..4248)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4297..4319
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4311..4328
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4312..4328
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4344..4443
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 atgctgagag aggaggccgc ccagaagaga aaggagaagg agcccggcat ggccctgcct
       61 cagggccacc tgacctttag agatgtggcc atcgagttca gcctggagga atggaagtgt
      121 ctggacccca cccagagagc cctgtacaga gccatgatgc tggagaacta cagaaacctg
      181 cacagcgtgg acatttccag caagtgcatg atgaagaagt tcagctccac agcccagggc
      241 aacaccgagg tggacaccgg caccctggag cggcacgaga gccatcacat cggcgacttc
      301 tgcttccaga agatcggaaa ggacatccac gactttgagt tccagtggca ggaggacaag
      361 agaaacagcc acgaggccac catgacccag atcaagaagc tgaccggcag caccgacaga
      421 tacgacagaa gacaccccgg caacaagccc atcaaggacc agctgggcct gagcttccac
      481 tcccatctgc ccgagctgca catcttccag accaagggga aggtgggcaa ccaggtggag
      541 aagagcatca acgacgccag ctccgtgctg accagccaga gaattagcag cagacccaag
      601 atccacatca gcaacaacta cgagaacaac ttctttcaca gctcactgct gaccctgaag
      661 caggaggtgc acatcagaga gaagtccttc caatgcaacg agtccggcaa agccttcaac
      721 tgcagctccc tgctgagaaa gcaccagatc atctacctgg gcggaaagca gtataagtgc
      781 gacgtgtgcg gaaaagtgtt caatcagaag aggtacctgg cctgccacca caggtgtcat
      841 accggcgaga aaccttacaa gtgcaacgaa tgcggcaaag tcttcaacca gcagagcaac
      901 ctggccagcc accaccggct gcacaccggt gaaaagccat acaagtgcga ggaatgcgat
      961 aaggtgttct cccggaagtc caacctggag agacacagaa gaatccatac cggagaaaag
     1021 ccctataaat gtaaggtgtg cgagaaggca ttcagaaggg acagccacct cacccagcac
     1081 accaggattc acacaggaga gaagccttac aaatgtaacg agtgtggcaa ggccttcagc
     1141 ggccagagca ccctgatcca ccaccaggcc atccatggca tcggcaagct gtacaagtgt
     1201 aacgactgcc acaaagtgtt tagcaacgcc accactatcg ccaaccactg gagaattcat
     1261 aacgaggaga ggagctacaa atgcaacaaa tgtggcaaat tcttcagaag aagaagctac
     1321 ctcgtggtgc actggaggac ccacacaggc gagaagccgt ataagtgtaa tgagtgtggg
     1381 aagaccttcc accacaacag cgccctggtg atccacaagg ctatccatac aggcgaaaag
     1441 ccttataagt gcaatgagtg cggcaagaca ttcagacaca actctgccct ggtcatccac
     1501 aaagccatcc acactggtga gaagccatat aaatgcaatg aatgcggaaa ggtgtttaat
     1561 cagcaggcca ccctggcccg gcaccacaga ctgcataccg gggagaagcc ctacaagtgt
     1621 gaggagtgcg acaccgtgtt cagcagaaag tctcaccatg agacacacaa aagaattcac
     1681 accggggaaa aaccttataa atgcgacgac ttcgacgagg cctttagcca ggccagcagc
     1741 tacgccaagc agagaaggat ccatatgggc gaaaaacacc acaaatgcga tgactgtgga
     1801 aaggctttca cctcccacag ccacagaatc agacaccaga ggatccacac cggccagaag
     1861 tcatacaaat gccacaagag aggcaaggtg ttttcctata cccagctttc ttgtacaaag
     1921 ttggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc
     1981 aaaataaaat cattatttgc catccagctg atatccccta tagtgagtcg tattacatgg
     2041 tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt tacattgcac
     2101 aagataaaaa tatatcatca tgaacaataa aactgtctgc ttacataaac agtaatacaa
     2161 ggggtgttat gagccatatt caacgggaaa cgtcgaggcc gcgattaaat tccaacatgg
     2221 atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa
     2281 tctatcgctt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta
     2341 gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg gaatttatgc
     2401 ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta ctcaccactg
     2461 cgatccccgg aaaaacagca ttccaggtat tagaagaata tcctgattca ggtgaaaata
     2521 ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc
     2581 cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg aataacggtt
     2641 tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa caagtctgga
     2701 aagaaatgca taaacttttg ccattctcac cggattcagt cgtcactcat ggtgatttct
     2761 cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat gttggacgag
     2821 tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc ggtgagtttt
     2881 ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct gatatgaata
     2941 aattgcagtt tcatttgatg ctcgatgagt ttttctaatc agaattggtt aattggttgt
     3001 aacactggca gagcattacg ctgacttgac gggacggcgc aagctcatga ccaaaatccc
     3061 ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     3121 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     3181 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     3241 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     3301 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     3361 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     3421 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     3481 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     3541 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     3601 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     3661 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     3721 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     3781 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     3841 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     3901 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     3961 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatacgcgt accgctagcc
     4021 aggaagagtt tgtagaaacg caaaaaggcc atccgtcagg atggccttct gcttagtttg
     4081 atgcctggca gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac
     4141 gttcaaatcc gctcccggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca
     4201 gataaaacga aaggcccagt cttccgactg agcctttcgt tttatttgat gcctggcagt
     4261 tccctactct cgcgttaacg ctagcatgga tgttttccca gtcacgacgt tgtaaaacga
     4321 cggccagtct taagctcggg ccccaaataa tgattttatt ttgactgata gtgacctgtt
     4381 cgttgcaaca aattgatgag caatgctttt ttataatgcc aactttgtac aaaaaagcag
     4441 gctta
//
