LOCUS       sequence-191353-        4304 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor Vector containing ZNF732 transcription factor, part of the 
            Human TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4304)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 4304)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR221-ZNF732
FEATURES             Location/Qualifiers
     source          1..4304
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1759..1858)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1875..1894)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1876..1894)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1899..1915)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1899..1915)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2017..2036)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2028..2837
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2095..2114)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2984..3572
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3473..3492
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3726..3743
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3902..3929)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4021..4107)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4156..4178
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4170..4187
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4171..4187
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4203..4302
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 atggagctgc tgaccttcag ggacgtggct atcgagttca gcccagagga gtggaagtgc
       61 ctggaccccg cccagcagaa cctgtacaga gacgtgatgc tggagaacta cagaaacctg
      121 atcagcctgg gcgtggccat cagcaacccc gacctggtga tctacctgga gcagaggaag
      181 gagccctaca aggtgaaaat ccatgagaca gtggccaagc accccgccgt gtgcagccac
      241 ttcacccagg acttcctgcc tgtgcagggc atcgaggaca gcttccacaa gctgatcctg
      301 agaaggtacg agaagtgcgg ccacgagaat ctggagctga gaaagagctg caagagaaag
      361 gtgcagaagg gcggctacaa cgagttcaac cagtgcctga gcaccatcca gagcaagatc
      421 ttccagtgca acgtgcatgt gaaggtgttt agcaccttca gcaacagcaa ccagagaaga
      481 atcaggcaca caggagagaa gcacttcaag gagtgcggta agagcttcca gaagttcagc
      541 gacctgaccc agcaccaggg aatccacgct ggggagaagc catacacatg cgaggaatgc
      601 ggcaaggact tcaagtggta cctgatcttc aacgagtacg agatcatcca cacgggcgaa
      661 aagccattca cttgtgagga atgtggtaac atcttcacca cctccagcaa cttcgcaaag
      721 cacaaggtgc acaccggcga gaagagctac aagtacgagg agtgcggcaa agcctttaac
      781 agaagcagca ccctgaccaa gcacaagagg atccacgccg aagagaagcc cttcacctgt
      841 gaagaatgtg gaaagatcat caccagcagc agcaacgtgg ccaaacataa aaaaatccac
      901 accggtgaga agctgtacaa gtgccaggaa tgtggcaaag tgttcaacag gagcacaacc
      961 ctgacaaaac acaacagaat ccataccggg gaaaaaccat acacttgcga ggaatgtggg
     1021 aaggcattca gcaggagcag cgtgctcaac gagcacaagc gcatccatac aggagaaaag
     1081 ccctataagt gcgagcagtg cggaaaagca tttagacaga gcgccaccct gaataaacac
     1141 aagagcattc acaccggaga gaaaccttat acctgcgaag agtgcgggaa ggcgttcagc
     1201 agattcacca cactgaacga acacaagcgg atccacactg gggagcggcc ccacaagtgc
     1261 gaagaatgcg gaaaggcctt cggctggtcc accgacctga acaagcataa aatcatacac
     1321 actggcgaga agccttacaa gtgtgaggag tgtgggaaag ccttcggatg gagcgcctac
     1381 ctgagcaaac acaaaaagat tcacactgga gaaaaacctt acagatgtga agagtgtggt
     1441 aaggccttcc tgtgctcccg ggctctgaat aagcacaaaa ctatccacac aggggagaaa
     1501 ccatatgagt gcgaggaatg cggtaaagct ttcgggtgga gcacctacct gtctaagcat
     1561 aagaagatac acacaggcga gaaaccctac cgctgcgagg aatgcgggaa agcattcaga
     1621 agaagcagag tgctgaacaa atacaagacc attcataccg gcgacaagac ccccaagtgc
     1681 aagggctgtg gcaaggcttt taagtggagc agctacctga accagcacaa caagatctat
     1741 acaggtgaga aactgtatac ccagctttct tgtacaaagt tggcattata agaaagcatt
     1801 gcttatcaat ttgttgcaac gaacaggtca ctatcagtca aaataaaatc attatttgcc
     1861 atccagctga tatcccctat agtgagtcgt attacatggt catagctgtt tcctggcagc
     1921 tctggcccgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat
     1981 gaacaataaa actgtctgct tacataaaca gtaatacaag gggtgttatg agccatattc
     2041 aacgggaaac gtcgaggccg cgattaaatt ccaacatgga tgctgattta tatgggtata
     2101 aatgggctcg cgataatgtc gggcaatcag gtgcgacaat ctatcgcttg tatgggaagc
     2161 ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag cgttgccaat gatgttacag
     2221 atgagatggt cagactaaac tggctgacgg aatttatgcc tcttccgacc atcaagcatt
     2281 ttatccgtac tcctgatgat gcatggttac tcaccactgc gatccccgga aaaacagcat
     2341 tccaggtatt agaagaatat cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt
     2401 tcctgcgccg gttgcattcg attcctgttt gtaattgtcc ttttaacagc gatcgcgtat
     2461 ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg agtgattttg
     2521 atgacgagcg taatggctgg cctgttgaac aagtctggaa agaaatgcat aaacttttgc
     2581 cattctcacc ggattcagtc gtcactcatg gtgatttctc acttgataac cttatttttg
     2641 acgaggggaa attaataggt tgtattgatg ttggacgagt cggaatcgca gaccgatacc
     2701 aggatcttgc catcctatgg aactgcctcg gtgagttttc tccttcatta cagaaacggc
     2761 tttttcaaaa atatggtatt gataatcctg atatgaataa attgcagttt catttgatgc
     2821 tcgatgagtt tttctaatca gaattggtta attggttgta acactggcag agcattacgc
     2881 tgacttgacg ggacggcgca agctcatgac caaaatccct taacgtgagt tacgcgtcgt
     2941 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     3001 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     3061 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     3121 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     3181 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     3241 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     3301 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     3361 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     3421 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     3481 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     3541 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     3601 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     3661 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     3721 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     3781 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     3841 gcagtgagcg caacgcaatt aatacgcgta ccgctagcca ggaagagttt gtagaaacgc
     3901 aaaaaggcca tccgtcagga tggccttctg cttagtttga tgcctggcag tttatggcgg
     3961 gcgtcctgcc cgccaccctc cgggccgttg cttcacaacg ttcaaatccg ctcccggcgg
     4021 atttgtccta ctcaggagag cgttcaccga caaacaacag ataaaacgaa aggcccagtc
     4081 ttccgactga gcctttcgtt ttatttgatg cctggcagtt ccctactctc gcgttaacgc
     4141 tagcatggat gttttcccag tcacgacgtt gtaaaacgac ggccagtctt aagctcgggc
     4201 cccaaataat gattttattt tgactgatag tgacctgttc gttgcaacaa attgatgagc
     4261 aatgcttttt tataatgcca actttgtaca aaaaagcagg ctta
//
