LOCUS       sequence-191355-        4376 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor Vector containing ZNF527 transcription factor, part of the 
            Human TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4376)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 4376)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR221-ZNF527
FEATURES             Location/Qualifiers
     source          1..4376
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1831..1930)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1947..1966)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1948..1966)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1971..1987)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1971..1987)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2089..2108)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2100..2909
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2167..2186)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3056..3644
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3545..3564
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3798..3815
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3974..4001)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4093..4179)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4228..4250
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4242..4259
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4243..4259
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4275..4374
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 atggccgtgg gcctgtgcaa ggccatgtca cagggcctgg tgaccttcag ggacgtggcc
       61 ctggactttt ctcaggagga gtgggaatgg ctgaagccca gccagaagga cctgtacaga
      121 gacgtgatgc tggagaacta cagaaacctg gtgtggctcg gcctgtccat cagcaagccc
      181 aacatgatca gcctgctgga acagggcaag gagccctgga tggtggaaag aaagatgagc
      241 cagggacact gcgccgactg ggagagctgg tgcgagatcg aggagctgag ccccaagtgg
      301 ttcatcgacg aggacgagat cagccaggag atggtgatgg agagactggc cagccacgga
      361 ctggagtgct ccagcttcag agaggcctgg aagtacaagg gcgagttcga gctgcaccag
      421 ggcaacgccg agaggcactt catgcaggtc accgccgtga aggagattag caccggcaaa
      481 agagacaacg agttcagcaa cagcggcaga agcatccccc tgaagagcgt gttcctgacc
      541 cagcagaagg tgcccaccat tcagcaggtc cacaagttcg acatctacga caagctgttc
      601 ccccagaaca gcgtgatcat cgagtacaag agactgcacg ctgagaagga gagcctgatc
      661 ggcaatgagt gcgaggagtt caaccagagc acctacctga gcaaggacat cggaatcccc
      721 cctggggaga agccctacga gagccacgac ttctccaagc tgctgagctt ccacagcctg
      781 tttacacagc atcaaaccac ccacttcggc aagctgcccc acggctacga cgaatgcggc
      841 gacgccttca gctgctacag cttcttcacc cagccacagc ggatccactc tggcgagaaa
      901 ccgtacgctt gcaacgactg tggcaaggca tttagccatg acttcttcct gagcgagcat
      961 cagagaaccc acatcggcga gaagccgtat gagtgcaagg agtgcaataa agctttcagg
     1021 cagagcgctc acctggccca acaccaaagg atacacaccg gcgaaaagcc cttcgcctgc
     1081 aacgaatgtg gcaaagcttt tagcagatac gccttcctgg tggaacatca gaggatccat
     1141 accggagaaa agccatatga gtgtaaggag tgtaacaagg ccttcagaca gagcgcccac
     1201 ctcaatcagc accagagaat tcacacaggt gagaagcctt acgagtgcaa ccagtgcggg
     1261 aaggctttct ccagaagaat cgccctgacc ctccaccaga ggattcacac cggtgagaaa
     1321 cctttcaagt gctccgagtg cggcaagacc ttcggctaca ggagccatct gaatcagcat
     1381 cagcggattc atacaggaga gaaaccctat gaatgcatca aatgcggcaa attcttcaga
     1441 accgacagcc agctgaacag acaccacagg atccacaccg gagagcggcc cttcgagtgc
     1501 agcaagtgcg gaaaggcttt ttccgacgcc ctggtcctga tccaccacaa gaggagccac
     1561 gccggggaga aaccatacga atgcaacaag tgtggtaagg cctttagctg cggcagctac
     1621 ctgaaccagc accaaagaat ccacacaggc gaaaaaccct acgaatgttc tgagtgtgga
     1681 aaggcattcc atcagatcct gagcctgagg ctgcatcaac ggatccatgc cggagagaag
     1741 ccatacaagt gcaacgagtg tggcaacaac ttctcctgcg tgagcgccct gagaagacac
     1801 cagcgcatcc acaacagaga gacactgtat acccagcttt cttgtacaaa gttggcatta
     1861 taagaaagca ttgcttatca atttgttgca acgaacaggt cactatcagt caaaataaaa
     1921 tcattatttg ccatccagct gatatcccct atagtgagtc gtattacatg gtcatagctg
     1981 tttcctggca gctctggccc gtgtctcaaa atctctgatg ttacattgca caagataaaa
     2041 atatatcatc atgaacaata aaactgtctg cttacataaa cagtaataca aggggtgtta
     2101 tgagccatat tcaacgggaa acgtcgaggc cgcgattaaa ttccaacatg gatgctgatt
     2161 tatatgggta taaatgggct cgcgataatg tcgggcaatc aggtgcgaca atctatcgct
     2221 tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca
     2281 atgatgttac agatgagatg gtcagactaa actggctgac ggaatttatg cctcttccga
     2341 ccatcaagca ttttatccgt actcctgatg atgcatggtt actcaccact gcgatccccg
     2401 gaaaaacagc attccaggta ttagaagaat atcctgattc aggtgaaaat attgttgatg
     2461 cgctggcagt gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt ccttttaaca
     2521 gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat gaataacggt ttggttgatg
     2581 cgagtgattt tgatgacgag cgtaatggct ggcctgttga acaagtctgg aaagaaatgc
     2641 ataaactttt gccattctca ccggattcag tcgtcactca tggtgatttc tcacttgata
     2701 accttatttt tgacgagggg aaattaatag gttgtattga tgttggacga gtcggaatcg
     2761 cagaccgata ccaggatctt gccatcctat ggaactgcct cggtgagttt tctccttcat
     2821 tacagaaacg gctttttcaa aaatatggta ttgataatcc tgatatgaat aaattgcagt
     2881 ttcatttgat gctcgatgag tttttctaat cagaattggt taattggttg taacactggc
     2941 agagcattac gctgacttga cgggacggcg caagctcatg accaaaatcc cttaacgtga
     3001 gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     3061 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     3121 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     3181 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     3241 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     3301 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     3361 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     3421 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     3481 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     3541 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     3601 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     3661 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     3721 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     3781 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     3841 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     3901 actggaaagc gggcagtgag cgcaacgcaa ttaatacgcg taccgctagc caggaagagt
     3961 ttgtagaaac gcaaaaaggc catccgtcag gatggccttc tgcttagttt gatgcctggc
     4021 agtttatggc gggcgtcctg cccgccaccc tccgggccgt tgcttcacaa cgttcaaatc
     4081 cgctcccggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac agataaaacg
     4141 aaaggcccag tcttccgact gagcctttcg ttttatttga tgcctggcag ttccctactc
     4201 tcgcgttaac gctagcatgg atgttttccc agtcacgacg ttgtaaaacg acggccagtc
     4261 ttaagctcgg gccccaaata atgattttat tttgactgat agtgacctgt tcgttgcaac
     4321 aaattgatga gcaatgcttt tttataatgc caactttgta caaaaaagca ggctta
//
