LOCUS       sequence-191359-        4571 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor Vector containing ZNF534 transcription factor, part of the 
            Human TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4571)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 4571)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR221-ZNF534
FEATURES             Location/Qualifiers
     source          1..4571
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(2026..2125)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2142..2161)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2143..2161)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2166..2182)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2166..2182)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2284..2303)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2295..3104
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2362..2381)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3251..3839
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3740..3759
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3993..4010
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4169..4196)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4288..4374)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4423..4445
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4437..4454
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4438..4454
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4470..4569
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 atggccctga cccagggaca gctgagcttc agcgacgtgg ccatcgagtt ctctcaggag
       61 gagtggaagt gcctggaccc cggccagaag gctctgtaca gggacgtgat gctggaaaac
      121 tacagaaacc tggtgtctct gggcgaggac aacgtgaggc cagaggcctg catttgcagc
      181 ggcatctgcc tgcccgacct gagcgtgacc tccatgctgg agcaaaagag ggacccttgg
      241 accctccagt ccgaggtgaa gatcatcaac aaccccgacg gcagagagtg cattaagggc
      301 gtgaacaccg agaagtcctc taagctgggc agcagcgccg gcaacaagag cctgaagaac
      361 cagcacggcc tgaccctgca gctgcacctg accgagtggc agcctttcca ggctgtgaga
      421 aacatctacg gctgcaagca cgtggagaag agcatcagcg acaacagcag cgtgagcccc
      481 gtgcagatca gcttcttcag cgtgaagacc cacatcttca acaactacag gaacgacttc
      541 ctgttcagca ccctcctgcc ccaggagcag aaggtgcaca tcagggagaa gccatacggg
      601 tgcaatgagc acgggaaagt ctttagggtg agcagctccc tgactaacag acaggtgatc
      661 cacatcgccg acaagaccta caagtgttct gactgcggcg agatcttcag ctccaactcc
      721 aacttcgcac agcaccaaag aattcatacc ggcgaaaagc cttataagta caacgaatgt
      781 ggaaaagtct tcaaccagaa ttcccacctt gcccagcacc agaaaatcca taccgggcaa
      841 aaaccctata acaacaagga atgtggcaag gtcttcagcc accacgccta cctggcccag
      901 cataggaaga tccacactgg cgagaaacca tacaagtgct cagagtgtgg caaagccttt
      961 agcgtgtgca gctctctgac cgcccatctg gtgattcaca caggcgagaa gccttacgac
     1021 tgtaaggaat gcggcaaagt gttcagacac aagagcagcc tgaccaccca ccagaccgtg
     1081 cataccggag agaggcccta taagtgtaac gagtgcggaa agggcttcag cagaatcgcc
     1141 ttcctggcta ggcacagaaa agtgcacacc ggggaaaagc cctacaaatg taacgaatgc
     1201 ggaaaggtat tcatcggaaa cagcagactg gccagacaca gaaagattca tactggcggc
     1261 agaagatata aatgcaatga gtgcgggaaa gccttcagga cctgcagcga cctcaccgcc
     1321 cacctcctga tccatacagg cgaaaaacct tacgagtgca tcgactgcgg aaaagtgttt
     1381 aggcacaagt cttccctgac ctaccactgt cgcattcaca cgggggaaaa accatataag
     1441 tgcaacgaat gcgggaaggt gttctcccag aacagcaacc tgcagaggca caggaagata
     1501 cataccggtg aaaagctgta taaatgtaat gagtgtggga aagttttcag acagaatagc
     1561 cacctcgctc agcatagaga catccacaca ggggagaaac cctacagctg caacgagtgt
     1621 ggaaaggtgt tccggagaaa cagccacctg gtgcgccaca gaaacgtgca cacaggagag
     1681 aagccgtact cctgtaatga atgtgggaag gtctttagca gaaactccca cctggcaaga
     1741 cacaggaata tccacaccgg tgagaagccc cactcttgca acgagtgcgg caaggttttc
     1801 agcaggaact ctcacctggc caggcataga aaaattcaca ctggggagaa gctgtacaaa
     1861 tgcaacgagt gtagcaaggt gttcagccgg aacagcaggc tggctcagca ccggaatatt
     1921 cacaccggcg tgaaacctta tagctgcaat gaatgcggta aggtgtttag caaaaacagc
     1981 atcctggtgc agcactgcag cattcataca agagaaaaac cctataccca gctttcttgt
     2041 acaaagttgg cattataaga aagcattgct tatcaatttg ttgcaacgaa caggtcacta
     2101 tcagtcaaaa taaaatcatt atttgccatc cagctgatat cccctatagt gagtcgtatt
     2161 acatggtcat agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca
     2221 ttgcacaaga taaaaatata tcatcatgaa caataaaact gtctgcttac ataaacagta
     2281 atacaagggg tgttatgagc catattcaac gggaaacgtc gaggccgcga ttaaattcca
     2341 acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg caatcaggtg
     2401 cgacaatcta tcgcttgtat gggaagcccg atgcgccaga gttgtttctg aaacatggca
     2461 aaggtagcgt tgccaatgat gttacagatg agatggtcag actaaactgg ctgacggaat
     2521 ttatgcctct tccgaccatc aagcatttta tccgtactcc tgatgatgca tggttactca
     2581 ccactgcgat ccccggaaaa acagcattcc aggtattaga agaatatcct gattcaggtg
     2641 aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta
     2701 attgtccttt taacagcgat cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata
     2761 acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct gttgaacaag
     2821 tctggaaaga aatgcataaa cttttgccat tctcaccgga ttcagtcgtc actcatggtg
     2881 atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt attgatgttg
     2941 gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac tgcctcggtg
     3001 agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat aatcctgata
     3061 tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctaatcagaa ttggttaatt
     3121 ggttgtaaca ctggcagagc attacgctga cttgacggga cggcgcaagc tcatgaccaa
     3181 aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc agaccccgta gaaaagatca
     3241 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     3301 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     3361 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     3421 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     3481 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     3541 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     3601 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     3661 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     3721 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     3781 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     3841 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     3901 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     3961 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     4021 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     4081 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat acgcgtaccg
     4141 ctagccagga agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt
     4201 agtttgatgc ctggcagttt atggcgggcg tcctgcccgc caccctccgg gccgttgctt
     4261 cacaacgttc aaatccgctc ccggcggatt tgtcctactc aggagagcgt tcaccgacaa
     4321 acaacagata aaacgaaagg cccagtcttc cgactgagcc tttcgtttta tttgatgcct
     4381 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
     4441 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
     4501 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
     4561 aagcaggctt a
//
