LOCUS       sequence-19136-e         720 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid was designed for screening purposes and may contain 
            discrepancies relative to the current canonical GenBank version of 
            the gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 720)
  AUTHORS   Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, 
            Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW
  TITLE     Identification of poxvirus CD8+ T cell determinants to enable 
            rational design and characterization of smallpox vaccines.
  JOURNAL   J Exp Med. 2005 January 3; 201(1): 95–104.
  PUBMED    15623576
REFERENCE   2  (bases 1 to 720)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "2005-?-3"; volume: " 201(1)"; pages: " 95–104"
COMMENT     Sequence Label: DCT.VV.Orf53
FEATURES             Location/Qualifiers
     source          1..720
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             498..539
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             549..566
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(589..606)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    595..706
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 gagctcggat ccagtaccct tcaccgaatt ccagctgacc accatgaaca gcactaccca
       61 tcacattagt tcccaatgga tcaatgtgtg taactccaga acatcttcca tatcctatgt
      121 taggaggagc gaacaccact cttccactat tgccatcgaa tgccatagaa taaatatcct
      181 tggaattgat agaaatcgga ctgtcggatg ttgtgatcat cttcatagga ttaacaacta
      241 tgtatggtgc cgcctgaagt ttcatatcgt aactgatgcc gtttataggt ctagccacag
      301 aaaccaacgt aggtctaaat ccaactatag acaaaataga agccaatatc tgttcctcat
      361 ctgtcataac ttgagagcat ccagtatgaa taatcttcat taggcaattc ccggggatcc
      421 gaataaaggg tcaagacaat tctgcagata tccagcacag tggcggccgc tcgagtctag
      481 agggcccgcg gttcgaaggt aagcctatcc ctaaccctct cctcggtctc gattctacgc
      541 gtaccggtca tcatcaccat caccattgag tttaaacccg ctgatcagcc tcgactgtgc
      601 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
      661 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
//
