LOCUS       sequence-19137-e         743 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid was designed for screening purposes and may contain 
            discrepancies relative to the current canonical GenBank version of 
            the gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, 
            Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW
  TITLE     Identification of poxvirus CD8+ T cell determinants to enable 
            rational design and characterization of smallpox vaccines.
  JOURNAL   J Exp Med. 2005 January 3; 201(1): 95–104.
  PUBMED    15623576
REFERENCE   2  (bases 1 to 743)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "2005-?-3"; volume: " 201(1)"; pages: " 95–104"
COMMENT     Sequence Label: DCT.VV.Orf54
FEATURES             Location/Qualifiers
     source          1..743
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             587..628
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             638..655
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(678..695)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 cagacacaga cgaatattat cgactatgaa tccgatgatc tcaccgaata cgaggatgat
       61 gaagaagagg aagaagaagg agagtcacta gaaactagtg atatagatcc caaatcttct
      121 tataagattg tagaatcagc atccactcat atagaagatg cgcattccaa tcttaaacat
      181 atagggaatc atatatctgc tcttaaacga cgctatacta gacgtataag tctatttgaa
      241 atagcgggta taatagcaga aagctataac ttgcttcaac gaggaagatt acctctagtt
      301 tcagaatttt ctgacgaaac gatgaagcaa aatatgctac atgtaattat acaagagata
      361 gaggagggtt cttgtcctat agtcatcgaa aagaacggag aattgttgtc ggtaaacgat
      421 tttgacaaag atggtctaaa attccatcta gactatatta tcaaaatttg gaaacttcaa
      481 aaacgatatt aggcaattcc cggggatccg aataaagggt caagacaatt ctgcagatat
      541 ccagcacagt ggcggccgct cgagtctaga gggcccgcgg ttcgaaggta agcctatccc
      601 taaccctctc ctcggtctcg attctacgcg taccggtcat catcaccatc accattgagt
      661 tttaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
      721 tcccccgtgc cttcctgacc ctg
//
