LOCUS       sequence-191371-        4430 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor Vector containing ZNF442 transcription factor, part of the 
            Human TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4430)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 4430)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR221-ZNF442
FEATURES             Location/Qualifiers
     source          1..4430
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1885..1984)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2001..2020)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2002..2020)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2025..2041)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2025..2041)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2143..2162)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2154..2963
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2221..2240)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3110..3698
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3599..3618
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3852..3869
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4028..4055)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4147..4233)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4282..4304
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4296..4313
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4297..4313
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4329..4428
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 atgatcgtgt tcggcggcga ggacagatct gacctgttcc tgcccgacag ccagaccaac
       61 gaggagagaa agcagtacga cagcgtggcc ttcgaggacg tggccgtgaa ctttacacag
      121 gaggagtggg ccctgctggg cccctcccag aagagcctgt acagagacgt gatgtgggaa
      181 accatcagaa acctggactg catcggcatg aagtgggagg acaccaacat cgaggaccag
      241 cacagaaacc ccagaaggag cctgagatgc cacatcatcg agagattcag cgagtccagg
      301 cagccagata gcaccgtgaa cgaaaagccc cccggcgtgg acccctgcaa aagcagcgtg
      361 tgcggcgaga ttatgggctg cagcttcctg aactgctaca tcacattcga cgccggccac
      421 aagcctgacg agtgccagga gtacggggaa aagcctcaca cccacaagca atgcggcacc
      481 gccttcaact accaccacag cttccagacc caggagaggc cccacacagg caagaagaga
      541 tacgactgta aggagtgtgg aaagaccttc tccagcagcg gcaacctgcg gagacatatc
      601 atcgtgcaga gaggcggcgg cccctacatc tgcaagctgt gcggaaaggc attcttctgg
      661 ccctccctgt tcaggatgca cgaaaggaca cacacgggag agaagcccta tgaatgtaaa
      721 cagtgctgca aagctttccc catctacagc tcctacctga gacacgagcg aactcacacc
      781 ggtgaaaagc catatgagtg caaacactgc tctaaggcct ttcccgacta cagcagctac
      841 gtgaggcacg aacgcaccca cacgggtgag aaaccgtaca aatgcaagag atgcggcaga
      901 gctttcagcg tgagcagctc cctgagaatc cacgagcgca cccataccgg cgagaagcca
      961 tacgagtgca agcagtgcgg taaagccttt caccacctgg gctctttcca gagacacatg
     1021 atcagacata caggcgacgg ccctcacaag tgcaagatct gtgggaaggg cttcgactgc
     1081 cccagctcac tgcagtccca cgagagaacc cacactggcg agaaacctta cgaatgtaag
     1141 cagtgtggca aggctctgag ccaccactca tccttcagat cccatatgat catgcacacc
     1201 ggagacggcc cccataagtg caaagtctgc ggcaaagcat tcatctaccc cagcgtgttc
     1261 cagggccatg agagaacaca cacaggggag aagccttatg agtgtaagga atgcggtaag
     1321 gctttcagaa tcagcagcag cctgaggaga catgagacta cacacaccgg cgaaaaacca
     1381 tacaagtgta agtgcggcaa ggccttcatc gacttctact ccttccagaa ccacgagaca
     1441 acccatacag gagaaaagcc ctacgagtgc aaggagtgcg ggaaggcttt tagctgcttc
     1501 acctacctta gccagcaccg gaggacccac atggcagaga aaccctacga atgcaaaacc
     1561 tgcaagaagg catttagcca cttcggaaac ctgaaggtgc acgagcggat tcacacaggt
     1621 gagaagccgt atgaatgcaa ggaatgtagg aaagccttca gctggctgac ctgcctgctg
     1681 cggcacgaga ggatccacac cgggaagaag tcttatgaat gccaacagtg cggaaaagca
     1741 tttaccagaa gcagattcct gagaggccac gagaagacac ataccgggga gaaaatgcac
     1801 gagtgtaaag aatgcgggaa agccctgtcc agcctgtcta gcctgcacag acacaagcgg
     1861 acccactgga gagacaccct gtatacccag ctttcttgta caaagttggc attataagaa
     1921 agcattgctt atcaatttgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta
     1981 tttgccatcc agctgatatc ccctatagtg agtcgtatta catggtcata gctgtttcct
     2041 ggcagctctg gcccgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat
     2101 catcatgaac aataaaactg tctgcttaca taaacagtaa tacaaggggt gttatgagcc
     2161 atattcaacg ggaaacgtcg aggccgcgat taaattccaa catggatgct gatttatatg
     2221 ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat cgcttgtatg
     2281 ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt gccaatgatg
     2341 ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt ccgaccatca
     2401 agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc cccggaaaaa
     2461 cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt gatgcgctgg
     2521 cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc
     2581 gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg
     2641 attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa atgcataaac
     2701 ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt gataacctta
     2761 tttttgacga ggggaaatta ataggttgta ttgatgttgg acgagtcgga atcgcagacc
     2821 gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct tcattacaga
     2881 aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg cagtttcatt
     2941 tgatgctcga tgagtttttc taatcagaat tggttaattg gttgtaacac tggcagagca
     3001 ttacgctgac ttgacgggac ggcgcaagct catgaccaaa atcccttaac gtgagttacg
     3061 cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     3121 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     3181 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     3241 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     3301 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     3361 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     3421 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     3481 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     3541 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     3601 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     3661 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     3721 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     3781 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     3841 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     3901 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     3961 aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa gagtttgtag
     4021 aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc tggcagttta
     4081 tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca aatccgctcc
     4141 cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa aacgaaaggc
     4201 ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct actctcgcgt
     4261 taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc agtcttaagc
     4321 tcgggcccca aataatgatt ttattttgac tgatagtgac ctgttcgttg caacaaattg
     4381 atgagcaatg cttttttata atgccaactt tgtacaaaaa agcaggctta
//
