LOCUS       sequence-19138-e         755 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid was designed for screening purposes and may contain 
            discrepancies relative to the current canonical GenBank version of 
            the gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, 
            Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW
  TITLE     Identification of poxvirus CD8+ T cell determinants to enable 
            rational design and characterization of smallpox vaccines.
  JOURNAL   J Exp Med. 2005 January 3; 201(1): 95–104.
  PUBMED    15623576
REFERENCE   2  (bases 1 to 755)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "2005-?-3"; volume: " 201(1)"; pages: " 95–104"
COMMENT     Sequence Label: DCT.VV.Orf55
FEATURES             Location/Qualifiers
     source          1..755
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             533..574
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             584..601
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(624..641)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    630..741
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ttggtaccga gctcggatcc agtacccttc accgaattcc agctgaccac catggcatta
       61 atggcaataa ctaaaaatgt aaaatatttt tctatgttag gaatatggtt tttcacttta
      121 atagatatat ggtttttggc caaaatgata gatatttttt tatccgagga tagtaaaata
      181 ttattagtcg ccgtctctat aaaaatgaag ctagtctcga tatccaattt tattctagaa
      241 ttgataggag tcgccaaatg taccttatac gttatatctc ccttgatgcg ttccatttgt
      301 gtatctatat cggacacaag atctgtaaat agttttacgt tattaatcgt cacggtatcg
      361 ccgtcgctag ataacgctaa tgtaccatcc aagtcccaaa tggagagatt taactgttca
      421 tcgtttagaa taaaatgagc aattcccggg gatccgaata aagggtcaag acaattctgc
      481 agatatccag cacagtggcg gccgctcgag tctagagggc ccgcggttcg aaggtaagcc
      541 tatccctaac cctctcctcg gtctcgattc tacgcgtacc ggtcatcatc accatcacca
      601 ttgagtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
      661 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
      721 ataaaatgag ggaattgcat cgcattgtct gagta
//
