LOCUS       sequence-191381-        4883 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor Vector containing ZNF749 transcription factor, part of the 
            Human TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4883)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 4883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR221-ZNF749
FEATURES             Location/Qualifiers
     source          1..4883
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(2338..2437)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2454..2473)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2455..2473)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2478..2494)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2478..2494)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2596..2615)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2607..3416
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2674..2693)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3563..4151
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4052..4071
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4305..4322
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4481..4508)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4600..4686)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4735..4757
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4749..4766
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4750..4766
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4782..4881
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 atgaacctga ccgaggactg catggtgttc gaggacgtgg ccatctattt cagccaggag
       61 gagtggggca ttctgaacga cgcccagaga catctgcaca gcaacgtcat gctggagaac
      121 tttgccctgc tgagcagcgt gggctgctgg cacggcgcca aggatgagga agtgcccagc
      181 aagcagtgtg tgagcgtgag agtgctccag gtgacaatcc ccaagcctgc cctgagcacc
      241 ctcaaggccc agccctgcaa gatgtgcagc agcatcctga aggacatcct gcacctggcc
      301 gaacacgacg gcacccatcc tgagcagggc ctgtatacat gcgccgccga gcacgacctg
      361 caccagaagg agcagatcag ggagaagctg accaggagcg acgagtggag gcctagcttc
      421 gtgaaccaca gcgctcacgt gggagagaga aacttcacct gtacccaggg cggaaaggac
      481 ttcaccgcct ccagcgatct gcttcagcag caggtcctca acagcggctg gaaactgtac
      541 agagacaccc aggacggcga ggctttccag ggcgagcaga acgacttcaa cagcagccag
      601 ggaggcaaag acttctgcca ccagcacggc ctgttcgagc atcagaagac ccacaacggc
      661 gagcgcccat acgagttcag cgagtgcggc gagctcttca ggtacaacag caacctcatc
      721 aagtaccagc agaaccacgc cggggagagg ccctacgaag gcaccgagta cggcaagacc
      781 ttcatcagga agagcaacct ggtccagcac caaaagattc acagcgaggg cttcctgtcc
      841 aagaggtccg accccatcga gcaccaggag atcctcagca gacccacccc ttatgagtgt
      901 acacagtgcg gcaaggcatt ccttacccag gcccatctgg tgggccatca gaaaacccac
      961 acaggagagc agccgtacga gtgcaataag tgtgggaagt tctttatgta caactccaaa
     1021 ctgatcagac atcaaaaagt gcacacaggt gagagaaggt acgaatgttc tgagtgcggg
     1081 aagctgttta tggacagctt caccctggga agacatcaga gggtgcacac cggtgagagg
     1141 cctttcgaat gctccatctg cggaaaattc ttcagccaca gaagcaccct gaacatgcac
     1201 cagcgggtgc acgccggaaa gagactgtac aagtgtagcg agtgtggtaa ggcctttagc
     1261 ctgaagcaca acgtcgtgca gcatctgaaa atccacactg gcgagcggcc ctatgagtgc
     1321 acagagtgcg agaaagcttt tgtgagaaaa agccacctcg tccagcatca aaagatccac
     1381 acagatgcct tcagcaagag atccgacctc atccagcata agagaatcga catcaggccc
     1441 cggccctaca cctgttcaga gtgtggcaag gccttcctca cccaagccca cctggtgggt
     1501 caccagaaga tccataccgg agagaggcca tatgaatgca cccagtgtgc caaggctttc
     1561 gtgagaaaga gccacttggt gcagcacgaa aaaatccata cagacgcctt ctccaagaga
     1621 agcgacctga tccagcacaa gagaattgac ctgaggccca gaccttacgt gtgcagcgaa
     1681 tgcggcaaag ccttcctgac ccaggctcat ctggacggcc accagaaaat tcagaccggc
     1741 gagagaagat acgaatgcaa tgagtgtgga aagtttttcc tggactccta taagctggtg
     1801 atccaccagc gcatccacac cggagaaaag ccctataagt gcagcaagtg cggaaagttc
     1861 ttcagataca gatgcaccct gtccagacac cagaaagttc acacaggcga aagaccctac
     1921 gagtgctctg aatgcggtaa atttttcaga gacagctaca agctgatcat ccatcagaga
     1981 gtgcataccg gcgaaaagcc ttacgagtgt agcaactgcg ggaaattcct gagatacagg
     2041 agcaccttca ttaagcacca caaggtgtgt actggagaga agccccacga gtgttccaag
     2101 tgcagagagc tgttcaggac caagtctagc ctgattatcc accaacagag ccacacgggc
     2161 gagagcccct tcaagctgag ggagtgcggt aaggacttta acaagtgcaa caccggccag
     2221 aggcagaaga cacatactgg cgaaaggagc tatgaatgtg gcgagtccag caaggtgttc
     2281 aagtacaata gcagcctgat caagcaccag atcattcaca ccggcaagag gccctatacc
     2341 cagctttctt gtacaaagtt ggcattataa gaaagcattg cttatcaatt tgttgcaacg
     2401 aacaggtcac tatcagtcaa aataaaatca ttatttgcca tccagctgat atcccctata
     2461 gtgagtcgta ttacatggtc atagctgttt cctggcagct ctggcccgtg tctcaaaatc
     2521 tctgatgtta cattgcacaa gataaaaata tatcatcatg aacaataaaa ctgtctgctt
     2581 acataaacag taatacaagg ggtgttatga gccatattca acgggaaacg tcgaggccgc
     2641 gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg
     2701 ggcaatcagg tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca gagttgtttc
     2761 tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc agactaaact
     2821 ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg
     2881 catggttact caccactgcg atccccggaa aaacagcatt ccaggtatta gaagaatatc
     2941 ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga
     3001 ttcctgtttg taattgtcct tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat
     3061 cacgaatgaa taacggtttg gttgatgcga gtgattttga tgacgagcgt aatggctggc
     3121 ctgttgaaca agtctggaaa gaaatgcata aacttttgcc attctcaccg gattcagtcg
     3181 tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt
     3241 gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga
     3301 actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg
     3361 ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt ttctaatcag
     3421 aattggttaa ttggttgtaa cactggcaga gcattacgct gacttgacgg gacggcgcaa
     3481 gctcatgacc aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg
     3541 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     3601 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     3661 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     3721 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     3781 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     3841 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     3901 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     3961 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     4021 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     4081 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     4141 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     4201 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     4261 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     4321 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     4381 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     4441 atacgcgtac cgctagccag gaagagtttg tagaaacgca aaaaggccat ccgtcaggat
     4501 ggccttctgc ttagtttgat gcctggcagt ttatggcggg cgtcctgccc gccaccctcc
     4561 gggccgttgc ttcacaacgt tcaaatccgc tcccggcgga tttgtcctac tcaggagagc
     4621 gttcaccgac aaacaacaga taaaacgaaa ggcccagtct tccgactgag cctttcgttt
     4681 tatttgatgc ctggcagttc cctactctcg cgttaacgct agcatggatg ttttcccagt
     4741 cacgacgttg taaaacgacg gccagtctta agctcgggcc ccaaataatg attttatttt
     4801 gactgatagt gacctgttcg ttgcaacaaa ttgatgagca atgctttttt ataatgccaa
     4861 ctttgtacaa aaaagcaggc tta
//
