LOCUS       sequence-19139-e         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid was designed for screening purposes and may contain 
            discrepancies relative to the current canonical GenBank version of 
            the gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, 
            Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW
  TITLE     Identification of poxvirus CD8+ T cell determinants to enable 
            rational design and characterization of smallpox vaccines.
  JOURNAL   J Exp Med. 2005 January 3; 201(1): 95–104.
  PUBMED    15623576
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "2005-?-3"; volume: " 201(1)"; pages: " 95–104"
COMMENT     Sequence Label: DCT.VV.Orf56
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             440..481
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             491..508
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(531..548)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    537..761
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 tcggatccag tacccttcac cgaattccag ctgaccacca tgatcttttt taacccagtt
       61 agttttagat tggtacgaaa tatattgtta aactttgatt ctacagtaat gtccaaatct
      121 agttgtggaa atacttccat caacattgtt tcaaacttga taatattatt atctacatct
      181 tcatacgatc caaattccgg aatagatgta tcacatgctc ttgccaccca gataaccaaa
      241 aagtcacacg ctccaggata tacattgtat aaaaagctat cgttttttag tagtgttttt
      301 ttctgagtat atacgaaagg attaaaaata gtattatcaa cgtaagcaat tcccggggat
      361 ccgaataaag ggtcaagaca attctgcaga tatccagcac agtggcggcc gctcgagtct
      421 agagggcccg cggttcgaag gtaagcctat ccctaaccct ctcctcggtc tcgattctac
      481 gcgtaccggt catcatcacc atcaccattg agtttaaacc cgctgatcag cctcgactgt
      541 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
      601 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
      661 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
      721 agacaatagc aggcatgctg gggatgcggt gggctctatg ggctctgagg cggaaagaac
      781 agctggggct ctagggggta
//
