LOCUS       sequence-191391-        4058 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor Vector containing ZNF730 transcription factor, part of the 
            Human TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4058)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 4058)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR221-ZNF730
FEATURES             Location/Qualifiers
     source          1..4058
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1513..1612)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1629..1648)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1630..1648)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1653..1669)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1653..1669)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1771..1790)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1782..2591
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1849..1868)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2738..3326
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3227..3246
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3480..3497
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3656..3683)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3775..3861)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3910..3932
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3924..3941
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3925..3941
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3957..4056
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 atgggcgccc tgaccttcag agacgtggcc atcgagttca gcctggagga gtggcagtgt
       61 ctggacaccg agcagcagaa cctgtacaga aacgtgatgc tggacaacta caggaacctg
      121 gtgttcctgg gcatcgccgt gagcaagccc gacctgatca catgcctgga gcaggagaag
      181 gagccttgga atctgaagac ccacgatatg gtggccaagc cccccgtgat ctgcagccac
      241 atcgcccagg atctgtggcc tgagcagggc atcaaggact acttccagga ggtgatcctg
      301 agacagtaca agaagtgcag acacgagaac ctgctgctga gaaagggctg caagaacgtg
      361 gacgagttca agatgcacaa aaagggctac aacagacaca accagtgcct gaccaccagc
      421 cacagcaaga tctttcagtg cgacaagtac gtgaaggtgt tccacaagtt cagcaacagc
      481 aaccggcata aaatcagaca caccagcaaa aagcccttca agtgcaagga gtgcgggaag
      541 ctgttctgca tcctgtccca cctggcccag cacaagaaga ttcacacagg cgaaaaaagc
      601 tacaagtgcg aagagtacgg caaggcattt aacgagagca gcaactgcac aacccacaag
      661 cggatcaccg agaagaaacc ctataaatgt aaagagtgtg ggaaggcttt caactggttc
      721 agccacttca ccacacacaa gaggatacac accggggaga agccctacca gtgcgagaag
      781 tgcggcaagt tcttcaacca gagcaccaat ctgaccactc acaagagaat ccacactggc
      841 gagaaacctt ataaatgcga ggagtgtggc aaagctttca atcagtccag caacctgacc
      901 gagcataaga agatccacac caaggagcag ccctacaaat gcgaaaagtg tggaaaggca
      961 ttcaagtggt ccagcaccct gaccaagcac aagcgcatcc acaacggaga gaaaccatac
     1021 aaatgtgaag aatgcggcaa agcatttaat agaagctcaa ccctgaacag acataagatc
     1081 acccacaccg gcggcaagcc ttacaagtac aaagaatgtg gaaaagcatt caaccaaagc
     1141 agcaccttga caatccacaa gatcattcac accgtggaga agttttataa gtgtgaagag
     1201 tgcggtaagg cctttagcag aatcagccac ctgactacac acaaaagaat ccatacagga
     1261 gagaagccat acaagtgtga ggaatgcgga agagccttta accagtcctc taccctgaca
     1321 actcacaaaa ggatccatac cggcgagaag ccttatgagt gcgaggaatg tggcaaggcc
     1381 ttcaacagaa gctccaccct caccacccac aaaatcatcc acagcggcga aaagatctat
     1441 aagtgcaaag agtgcggaaa ggcttttaga aggttctctc acctgaccag gcacaagacc
     1501 atccacacat atacccagct ttcttgtaca aagttggcat tataagaaag cattgcttat
     1561 caatttgttg caacgaacag gtcactatca gtcaaaataa aatcattatt tgccatccag
     1621 ctgatatccc ctatagtgag tcgtattaca tggtcatagc tgtttcctgg cagctctggc
     1681 ccgtgtctca aaatctctga tgttacattg cacaagataa aaatatatca tcatgaacaa
     1741 taaaactgtc tgcttacata aacagtaata caaggggtgt tatgagccat attcaacggg
     1801 aaacgtcgag gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg
     1861 ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg aagcccgatg
     1921 cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga
     1981 tggtcagact aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc
     2041 gtactcctga tgatgcatgg ttactcacca ctgcgatccc cggaaaaaca gcattccagg
     2101 tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca gtgttcctgc
     2161 gccggttgca ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc
     2221 tcgctcaggc gcaatcacga atgaataacg gtttggttga tgcgagtgat tttgatgacg
     2281 agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat gcataaactt ttgccattct
     2341 caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg
     2401 ggaaattaat aggttgtatt gatgttggac gagtcggaat cgcagaccga taccaggatc
     2461 ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa cggctttttc
     2521 aaaaatatgg tattgataat cctgatatga ataaattgca gtttcatttg atgctcgatg
     2581 agtttttcta atcagaattg gttaattggt tgtaacactg gcagagcatt acgctgactt
     2641 gacgggacgg cgcaagctca tgaccaaaat cccttaacgt gagttacgcg tcgttccact
     2701 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     2761 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     2821 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     2881 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     2941 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     3001 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     3061 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     3121 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     3181 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     3241 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     3301 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     3361 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     3421 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     3481 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     3541 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     3601 agcgcaacgc aattaatacg cgtaccgcta gccaggaaga gtttgtagaa acgcaaaaag
     3661 gccatccgtc aggatggcct tctgcttagt ttgatgcctg gcagtttatg gcgggcgtcc
     3721 tgcccgccac cctccgggcc gttgcttcac aacgttcaaa tccgctcccg gcggatttgt
     3781 cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc agtcttccga
     3841 ctgagccttt cgttttattt gatgcctggc agttccctac tctcgcgtta acgctagcat
     3901 ggatgttttc ccagtcacga cgttgtaaaa cgacggccag tcttaagctc gggccccaaa
     3961 taatgatttt attttgactg atagtgacct gttcgttgca acaaattgat gagcaatgct
     4021 tttttataat gccaactttg tacaaaaaag caggctta
//
