LOCUS       sequence-191393-        4046 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor Vector containing ZNF727 transcription factor, part of the 
            Human TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4046)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 4046)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR221-ZNF727
FEATURES             Location/Qualifiers
     source          1..4046
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1501..1600)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1617..1636)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1618..1636)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1641..1657)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1641..1657)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1759..1778)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1770..2579
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1837..1856)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2726..3314
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3215..3234
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3468..3485
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3644..3671)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3763..3849)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3898..3920
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3912..3929
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3913..3929
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3945..4044
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 atgagagtgc tgaccttcag agacgtggcc gtggagttca gcccagagga gtgggagtgc
       61 ctggacagcg cccagcagag actgtacagg gacgtgatgc tggagaacta cggcaacctg
      121 ttcagcctgg gcctggccat ctttaagcca gacctgatca cctacctgga gcagagaaag
      181 gagccctgga acgccaggag acagaagacc gtggctaagc accccgccgg cagcctgcac
      241 tttaccgccg agatcctgct ggagcacgac atcaacgaca gcttccagaa ggtgatcctg
      301 agaaagagcg gcagctgcga cctgaacacc ctgagactga agaaggacta ccagagagtg
      361 ggcaactgca agggccagaa gagcagctac aacggcatcc accagtgcct gagcgccacc
      421 agaagcaaga catgccagta caacaaatgc ggcaaagcct tcggcctgtg ctccatcttc
      481 acagagcaca agaaaatctt ctccagagag aagtgctata aatgcgaaga gtgtggcaag
      541 gactgcagac tgagcgactt caccattcag aagagaattc acacagccga cagaagctat
      601 aagtgtgaag agtgcgggaa ggcctgcaag aagttcagca acctgaccga gcacaaccgg
      661 gtgcacaccg gaaagaagcc ttacaaatgc gaggagtgtg ggaagacatt tacctgcagc
      721 agcgccctga ctaagcataa gaggaaccac actggcgaca gaccctacaa gtgtgaggag
      781 tgtcacaagg cctttaggtg ctgctctgac ctgaccaaac ataagagaat ccacacaggc
      841 gagaaaccat acaagtgcaa ggagtgccac aaggctttca gatgctgctc cgacctgaca
      901 aaacacaagc gcattcacac cggggagaaa ccttataagt gcaacgagtg cggtaaggcc
      961 ttcatgtgga tctccgccct gagccagcac aacaggatcc atacaggaga gaagccctac
     1021 atctgtgagg aatgcgggaa agcattcacc tactccagca ccctgatcag ccacaagcga
     1081 attcatatgg agctcagacc atataagtgc gaagaatgtg gaaagacctt caaatggttc
     1141 agcgacctca caaaccacaa aagaatccat accggagaaa aaccttacaa gtgcgaggaa
     1201 tgtggcaaaa gcttcacctg tagcagcaat ctgatcaagc acaagcggat tcacatggag
     1261 gtgaggccct acaaatgtga agaatgcgga aagacattca agtggttccc cgatctgacc
     1321 aaccacaaga ggatccacac cggcgagaag ccatataaat gtgaggagtg cggcaagacc
     1381 tttacctgtt ccagcagcct gatcaaacac aaaaggtcac ataccggcga caggcccacc
     1441 agcgccaaga acgtggccaa gcccctgggc ggcagccaga cactgctgaa catcagatat
     1501 acccagcttt cttgtacaaa gttggcatta taagaaagca ttgcttatca atttgttgca
     1561 acgaacaggt cactatcagt caaaataaaa tcattatttg ccatccagct gatatcccct
     1621 atagtgagtc gtattacatg gtcatagctg tttcctggca gctctggccc gtgtctcaaa
     1681 atctctgatg ttacattgca caagataaaa atatatcatc atgaacaata aaactgtctg
     1741 cttacataaa cagtaataca aggggtgtta tgagccatat tcaacgggaa acgtcgaggc
     1801 cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg
     1861 tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa gcccgatgcg ccagagttgt
     1921 ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg gtcagactaa
     1981 actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt actcctgatg
     2041 atgcatggtt actcaccact gcgatccccg gaaaaacagc attccaggta ttagaagaat
     2101 atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc cggttgcatt
     2161 cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc
     2221 aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag cgtaatggct
     2281 ggcctgttga acaagtctgg aaagaaatgc ataaactttt gccattctca ccggattcag
     2341 tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg aaattaatag
     2401 gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt gccatcctat
     2461 ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa aaatatggta
     2521 ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag tttttctaat
     2581 cagaattggt taattggttg taacactggc agagcattac gctgacttga cgggacggcg
     2641 caagctcatg accaaaatcc cttaacgtga gttacgcgtc gttccactga gcgtcagacc
     2701 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     2761 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     2821 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     2881 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     2941 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     3001 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     3061 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     3121 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     3181 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     3241 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     3301 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     3361 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     3421 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     3481 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     3541 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     3601 ttaatacgcg taccgctagc caggaagagt ttgtagaaac gcaaaaaggc catccgtcag
     3661 gatggccttc tgcttagttt gatgcctggc agtttatggc gggcgtcctg cccgccaccc
     3721 tccgggccgt tgcttcacaa cgttcaaatc cgctcccggc ggatttgtcc tactcaggag
     3781 agcgttcacc gacaaacaac agataaaacg aaaggcccag tcttccgact gagcctttcg
     3841 ttttatttga tgcctggcag ttccctactc tcgcgttaac gctagcatgg atgttttccc
     3901 agtcacgacg ttgtaaaacg acggccagtc ttaagctcgg gccccaaata atgattttat
     3961 tttgactgat agtgacctgt tcgttgcaac aaattgatga gcaatgcttt tttataatgc
     4021 caactttgta caaaaaagca ggctta
//
