LOCUS       sequence-191405-        4160 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor Vector containing ZNF835 transcription factor, part of the 
            Human TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4160)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 4160)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR221-ZNF835
FEATURES             Location/Qualifiers
     source          1..4160
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1615..1714)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1731..1750)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1732..1750)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1755..1771)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1755..1771)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1873..1892)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1884..2693
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1951..1970)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2840..3428
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3329..3348
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3582..3599
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3758..3785)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3877..3963)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4012..4034
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4026..4043
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4027..4043
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4059..4158
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 atggagggcc tgctgagcgt ggccctgcaa ggcgccgagc tggagggcaa ctggaagcac
       61 gagggccagg tggaggatct gcaggagaat caggagtctt gccctgaacc cgaggccgtg
      121 gcctgcaaag gcgatcccgc cggcgatagc atgcaggagc gggacgagtt cagcagaatc
      181 cccagaacca tcagctctcc cgccgctacc caggccagcg tgcctgatga cagctccagc
      241 agaagatgta gcgcccctgg cgagtccccc aaggagagac accctgacag cagacagagg
      301 gagagaggcg gcggccccaa gaagccttgg aagtgcggag actgtggcaa ggctttcagc
      361 tactgctccg ccttcatcct gcatcagcgg attcataccg gagaaaagcc cttcgcctgc
      421 cctgagtgtg gaaaagcatt cagccagagc gtgcacctca cactccatca gagaacccac
      481 accggagaga aaccttacgc ctgtcacgag tgcgggaagg ccttctctca gggctcctac
      541 ctggccagcc actggagaac acacacagga gagaagcctc acagatgcgc cgactgtggt
      601 aaggccttca ccagagtgac ccacctgaca cagcatagaa gagtccacac aggtgagagg
      661 ccctacgcct gcgcccagtg cgccaaggca tttcggaata gaagctccct gatcgagcac
      721 cagcggatcc acactggcga gaagccctac gaatgcagcg cctgtgctaa agcctttaga
      781 ttcagcagcg ccctgatcag acaccagaga attcacacag aggagaaacc ctatagatgc
      841 ggacagtgcg ctaaggcatt cgcccagatc gcccatctga cccagcacag gagggtgcat
      901 accggcgaaa agccttatac ctgccaggac tgcggcgccc tgttctccca gagcgcctca
      961 ctggccgagc acagaagaat ccatacaggc gagaaaccat atgcctgcgg ccaatgcgcc
     1021 aaagctttca cccaggtgag ccacctcacc cagcatcaga ggacccatac cggggaacgc
     1081 ccctacccct gccacgactg cggaaaaaga tttagcaaca gaagccacct tctgcagcac
     1141 agactggtgc acaccgggga gcggccctac agatgcctgc agtgtggcgc cgccttctcc
     1201 cacgtgagca gcctcatcga acaccagaag attcacaccg gcgagaggcc atacaagtgc
     1261 ggggagtgcg gaaaggcatt tagccagggc agcagcctgg ctctgcacca aagaactcac
     1321 acaggcgaaa gaccctacac atgccccgag tgtggcaaag ccttcagcaa caggagctac
     1381 ctgatccagc accacatcgt gcacacaggg gagaagccat acgagtgcag cggctgcggc
     1441 aaggccttta gcttcagctc agccctgatt aggcaccaga ggacacacgc cgacagcagc
     1501 ggcagactgt gccctgctcc cacacccgat agcactcctg gcctgtctca gggaggcgag
     1561 acatgtcagc agggctgtcc aggcagaaat cccagaggcc ccgccgagga ctatacccag
     1621 ctttcttgta caaagttggc attataagaa agcattgctt atcaatttgt tgcaacgaac
     1681 aggtcactat cagtcaaaat aaaatcatta tttgccatcc agctgatatc ccctatagtg
     1741 agtcgtatta catggtcata gctgtttcct ggcagctctg gcccgtgtct caaaatctct
     1801 gatgttacat tgcacaagat aaaaatatat catcatgaac aataaaactg tctgcttaca
     1861 taaacagtaa tacaaggggt gttatgagcc atattcaacg ggaaacgtcg aggccgcgat
     1921 taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc
     1981 aatcaggtgc gacaatctat cgcttgtatg ggaagcccga tgcgccagag ttgtttctga
     2041 aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc
     2101 tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat
     2161 ggttactcac cactgcgatc cccggaaaaa cagcattcca ggtattagaa gaatatcctg
     2221 attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc
     2281 ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac
     2341 gaatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg
     2401 ttgaacaagt ctggaaagaa atgcataaac ttttgccatt ctcaccggat tcagtcgtca
     2461 ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta
     2521 ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact
     2581 gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata
     2641 atcctgatat gaataaattg cagtttcatt tgatgctcga tgagtttttc taatcagaat
     2701 tggttaattg gttgtaacac tggcagagca ttacgctgac ttgacgggac ggcgcaagct
     2761 catgaccaaa atcccttaac gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag
     2821 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     2881 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     2941 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     3001 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     3061 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     3121 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     3181 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     3241 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     3301 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     3361 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     3421 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     3481 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     3541 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     3601 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     3661 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata
     3721 cgcgtaccgc tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc
     3781 cttctgctta gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg
     3841 ccgttgcttc acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt
     3901 caccgacaaa caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat
     3961 ttgatgcctg gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac
     4021 gacgttgtaa aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac
     4081 tgatagtgac ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt
     4141 tgtacaaaaa agcaggctta
//
