LOCUS       sequence-19141-e         768 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid was designed for screening purposes and may contain 
            discrepancies relative to the current canonical GenBank version of 
            the gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, 
            Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW
  TITLE     Identification of poxvirus CD8+ T cell determinants to enable 
            rational design and characterization of smallpox vaccines.
  JOURNAL   J Exp Med. 2005 January 3; 201(1): 95–104.
  PUBMED    15623576
REFERENCE   2  (bases 1 to 768)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "2005-?-3"; volume: " 201(1)"; pages: " 95–104"
COMMENT     Sequence Label: DCT.VV.Orf58
FEATURES             Location/Qualifiers
     source          1..768
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             633..674
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             684..701
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(724..741)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 ggatccagta cccttcaccg aattccagct gaccaccatg gatgtagcaa gatttttaaa
       61 caacaatgcc atcttacacg atgattgcct caagtctcca atcttttgtt tagaacgatt
      121 agctacagag ttcaacgctt ggctgactag catattatta tctttagaaa ttgtattctt
      181 caatgaggcg tttatcatat ctgtgatttc gttagtcata ttacagtctg actgggttgt
      241 aatgttatcc aacatatcac ctatggatac ggtacacgta ccagcatttg taataatcct
      301 atctaagatg ttgtatggca ttgcgcagaa aatatcttct cctgtaatat ctccactctc
      361 gataaatcta ctcagattat tcttaaatgc cttattctct ggagaaaaga tatcagtgtc
      421 catcatttca ttaatagtat acgcagaaaa gataccacga gtatcaattc tatccaagat
      481 acttatcggt tccgagtcac agataatggt ttcctctcct tcgggagatc ctgcataggc
      541 aattcccggg gatccgaata aagggtcaag acaattctgc agatatccag cacagtggcg
      601 gccgctcgag tctagagggc ccgcggttcg aaggtaagcc tatccctaac cctctcctcg
      661 gtctcgattc tacgcgtacc ggtcatcatc accatcacca ttgagtttaa acccgctgat
      721 cagcctcgac tgtgccttct agttgccacc catctgttgt ttgcccct
//
