LOCUS       sequence-191411-        4043 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor Vector containing ZNF682 transcription factor, part of the 
            Human TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4043)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 4043)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR221-ZNF682
FEATURES             Location/Qualifiers
     source          1..4043
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1498..1597)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1614..1633)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1615..1633)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1638..1654)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1638..1654)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1756..1775)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1767..2576
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1834..1853)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2723..3311
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3212..3231
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3465..3482
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3641..3668)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3760..3846)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3895..3917
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3909..3926
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3910..3926
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3942..4041
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 atggagctgc tgacattcag agacgtgacc atcgagttca gcctggaaga gtgggagttc
       61 ctgaaccccg cccagcagag cctgtacaga aaggtgatgc tggagaacta cagaaacctg
      121 gtgagcctgg gcctgaccgt gagcaagccc gaactgatca gcagactgga gcagagacag
      181 gagccttgga acgtgaagag acacgaaacc atcgccaagc cccctgccat gagcagccac
      241 tacaccgagg acctgctgcc cgagcagtgt atgcaggaca gcttccagaa ggtcatcctg
      301 agaagatacg gcagctgcgg cctggaggat ctgcacctga gaaaggacgg cgaaaacgtg
      361 ggcgagtgca aagaccagaa ggagatctac aacggcctga accagtgcct gagcaccctg
      421 cccagcaaga tcttccccta caacaagtgc gtgaaggtgt tcagcaagag ctccaacctg
      481 aacagggaga acatcagaca caccaccgag aagctcttta agtgcatgca gtgtggcaaa
      541 gtgttcaaga gccacagcgg cctgagctac cacaagatca tccataccga ggagaagctg
      601 tgcatctgcg aagaatgcgg aaagaccttc aagtggttca gctatctgac taagcataag
      661 agaattcaca caggagagaa accatacaaa tgtgaggagt gtggtaaggc tttcaactgg
      721 tgcagcagcc tgaccaagca caaaagaatc catacaggcg agaagcccta taaatgcgaa
      781 gagtgtggaa aggccttcca ctggtgctct cccttcgtga gacacaagaa gattcacacc
      841 ggcgagaaac cttacacttg cgaggactgc ggcagagctt tcaatagaca ctctcacctg
      901 actaaacaca agacaatcca cacagggaag aaaccctaca agtgcaagga atgtggcaag
      961 gcattcaacc actgctccct gctgaccatc cacgagagaa cacacacagg cgaaaaacct
     1021 tataagtgcg aggaatgcgg caaagccttc aactccagct ctatcctgac cgagcataag
     1081 gtgatccact ctggcgaaaa gccatacaag tgtgagaagt gcgacaaggt gtttaagaga
     1141 ttcagctacc tgacaaagca caagaggatc cacaccggag aaaaacccta taagtgtgaa
     1201 gagtgcggaa aagcattcaa ttggagcagc atcctgacag agcacaaacg cattcataca
     1261 ggagaaaagc cctacaattg tgaagaatgt gggaaggcat ttaacagatg cagccacctg
     1321 acccggcaca aaaagattca taccgccgtg aagcggtaca aatgcgagga gtgcggcaag
     1381 gcttttaaga ggtgctccca cctgaacgag cacaagcggg tgcagagggg agagaagagc
     1441 tgcaagtaca agaagtgtgg cgaggccttc aatcactgca gcaacctgac cacctatacc
     1501 cagctttctt gtacaaagtt ggcattataa gaaagcattg cttatcaatt tgttgcaacg
     1561 aacaggtcac tatcagtcaa aataaaatca ttatttgcca tccagctgat atcccctata
     1621 gtgagtcgta ttacatggtc atagctgttt cctggcagct ctggcccgtg tctcaaaatc
     1681 tctgatgtta cattgcacaa gataaaaata tatcatcatg aacaataaaa ctgtctgctt
     1741 acataaacag taatacaagg ggtgttatga gccatattca acgggaaacg tcgaggccgc
     1801 gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg
     1861 ggcaatcagg tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca gagttgtttc
     1921 tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc agactaaact
     1981 ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg
     2041 catggttact caccactgcg atccccggaa aaacagcatt ccaggtatta gaagaatatc
     2101 ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga
     2161 ttcctgtttg taattgtcct tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat
     2221 cacgaatgaa taacggtttg gttgatgcga gtgattttga tgacgagcgt aatggctggc
     2281 ctgttgaaca agtctggaaa gaaatgcata aacttttgcc attctcaccg gattcagtcg
     2341 tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt
     2401 gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga
     2461 actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg
     2521 ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt ttctaatcag
     2581 aattggttaa ttggttgtaa cactggcaga gcattacgct gacttgacgg gacggcgcaa
     2641 gctcatgacc aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg
     2701 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     2761 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     2821 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     2881 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     2941 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     3001 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     3061 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     3121 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     3181 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     3241 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     3301 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     3361 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     3421 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     3481 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     3541 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     3601 atacgcgtac cgctagccag gaagagtttg tagaaacgca aaaaggccat ccgtcaggat
     3661 ggccttctgc ttagtttgat gcctggcagt ttatggcggg cgtcctgccc gccaccctcc
     3721 gggccgttgc ttcacaacgt tcaaatccgc tcccggcgga tttgtcctac tcaggagagc
     3781 gttcaccgac aaacaacaga taaaacgaaa ggcccagtct tccgactgag cctttcgttt
     3841 tatttgatgc ctggcagttc cctactctcg cgttaacgct agcatggatg ttttcccagt
     3901 cacgacgttg taaaacgacg gccagtctta agctcgggcc ccaaataatg attttatttt
     3961 gactgatagt gacctgttcg ttgcaacaaa ttgatgagca atgctttttt ataatgccaa
     4021 ctttgtacaa aaaagcaggc tta
//
